ID   CRAS2_URTCR             Reviewed;          34 AA.
AC   P0DUG3;
DT   07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT   07-APR-2021, sequence version 1.
DT   25-MAY-2022, entry version 5.
DE   RecName: Full=Crassicorin-II {ECO:0000303|PubMed:28796463};
DE   AltName: Full=BDS-like antimicrobial peptide {ECO:0000303|PubMed:28796463};
DE            Short=BDS-like AMP {ECO:0000303|PubMed:28796463};
DE   Flags: Fragment;
OS   Urticina crassicornis (Mottled anemone) (Tealia crassicornis).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC   Actiniidae; Urticina.
OX   NCBI_TaxID=45621;
RN   [1]
RP   PROTEIN SEQUENCE, AND MASS SPECTROMETRY.
RX   PubMed=28796463; DOI=10.1111/febs.14194;
RA   Kim C.H., Lee Y.J., Go H.J., Oh H.Y., Lee T.K., Park J.B., Park N.G.;
RT   "Defensin-neurotoxin dyad in a basally branching metazoan sea anemone.";
RL   FEBS J. 284:3320-3338(2017).
CC   -!- FUNCTION: Peptide with both antimicrobial and neurotoxin activities.
CC       Cationic AMP with antimicrobial activity against both Gram-positive
CC       bacteria (B.subtilis) and Gram-negative bacteria (E.coli and
CC       S.enterica). Shows no significant antimicrobial activity against
CC       bacteria S.aureus and P.aeruginosa, as well as the fungus C.albicans.
CC       In vivo, induces reversible paralytic activity towards the shrimp
CC       P.paucidens. May act by impairing sodium or potassium channels in the
CC       prey. {ECO:0000250|UniProtKB:A0A1X9QHL1}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:A0A1X9QHL1}.
CC       Nematocyst {ECO:0000250|UniProtKB:A0A1X9QHL1}.
CC   -!- TISSUE SPECIFICITY: Highly expressed by the mesenteries. Moderately
CC       expressed by the pharynx. Weakly expressed by the gonad and pedal disk.
CC       No expression in tentacle. {ECO:0000250|UniProtKB:A0A1X9QHL1}.
CC   -!- MASS SPECTROMETRY: Mass=4386.4; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:28796463};
CC   -!- SIMILARITY: Belongs to the sea anemone type 3 (BDS) potassium channel
CC       toxin family. {ECO:0000305}.
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DR   AlphaFoldDB; P0DUG3; -.
DR   SMR; P0DUG3; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   Gene3D; 2.20.20.10; -; 1.
DR   InterPro; IPR012414; BDS_K_chnl_tox.
DR   InterPro; IPR023355; Myo_ane_neurotoxin_sf.
DR   Pfam; PF07936; Defensin_4; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Cleavage on pair of basic residues;
KW   Direct protein sequencing; Disulfide bond; Immunity; Innate immunity;
KW   Ion channel impairing toxin; Nematocyst; Neurotoxin; Secreted; Toxin.
FT   CHAIN           1..>34
FT                   /note="Crassicorin-II"
FT                   /evidence="ECO:0000269|PubMed:28796463"
FT                   /id="PRO_0000452125"
FT   DISULFID        4..?
FT                   /evidence="ECO:0000250|UniProtKB:P61541"
FT   DISULFID        6..30
FT                   /evidence="ECO:0000250|UniProtKB:P61541"
FT   DISULFID        20..?
FT                   /evidence="ECO:0000250|UniProtKB:P61541"
FT   NON_TER         34
SQ   SEQUENCE   34 AA;  3792 MW;  D513CBDDC9BA5614 CRC64;
     GASCDCHPFK GTYWFGTSNC PSGHGYRKKC DSFF