ID   CRA_ECO57               Reviewed;         334 AA.
AC   P0ACP3; P21168;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Catabolite repressor/activator;
DE   AltName: Full=Fructose repressor;
GN   Name=cra; Synonyms=fruR; OrderedLocusNames=Z0090, ECs0084;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Global transcriptional regulator, which plays an important
CC       role in the regulation of carbon metabolism. {ECO:0000250}.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
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DR   EMBL; AE005174; AAG54384.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB33507.1; -; Genomic_DNA.
DR   PIR; D85490; D85490.
DR   PIR; D90639; D90639.
DR   RefSeq; NP_308111.1; NC_002695.1.
DR   RefSeq; WP_000762401.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P0ACP3; -.
DR   SMR; P0ACP3; -.
DR   STRING; 155864.EDL933_0083; -.
DR   EnsemblBacteria; AAG54384; AAG54384; Z0090.
DR   EnsemblBacteria; BAB33507; BAB33507; ECs_0084.
DR   GeneID; 67416153; -.
DR   GeneID; 913527; -.
DR   KEGG; ece:Z0090; -.
DR   KEGG; ecs:ECs_0084; -.
DR   PATRIC; fig|386585.9.peg.184; -.
DR   eggNOG; COG1609; Bacteria.
DR   HOGENOM; CLU_037628_6_0_6; -.
DR   OMA; NSRKAGY; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0009750; P:response to fructose; IEA:InterPro.
DR   CDD; cd01392; HTH_LacI; 1.
DR   Gene3D; 1.10.260.40; -; 1.
DR   InterPro; IPR012781; Fruct_sucro_rep.
DR   InterPro; IPR000843; HTH_LacI.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR001761; Peripla_BP/Lac1_sug-bd_dom.
DR   InterPro; IPR028082; Peripla_BP_I.
DR   Pfam; PF00356; LacI; 1.
DR   Pfam; PF00532; Peripla_BP_1; 1.
DR   SMART; SM00354; HTH_LACI; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
DR   SUPFAM; SSF53822; SSF53822; 1.
DR   TIGRFAMs; TIGR02417; fruct_sucro_rep; 1.
DR   PROSITE; PS00356; HTH_LACI_1; 1.
DR   PROSITE; PS50932; HTH_LACI_2; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..334
FT                   /note="Catabolite repressor/activator"
FT                   /id="PRO_0000107946"
FT   DOMAIN          1..58
FT                   /note="HTH lacI-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00111"
FT   DNA_BIND        3..22
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00111"
SQ   SEQUENCE   334 AA;  37999 MW;  503AE935CC2EC0C8 CRC64;
     MKLDEIARLA GVSRTTASYV INGKAKQYRV SDKTVEKVMA VVREHNYHPN AVAAGLRAGR
     TRSIGLVIPD LENTSYTRIA NYLERQARQR GYQLLIACSE DQPDNEMRCI EHLLQRQVDA
     IIVSTSLPPE HPFYQRWAND PFPIVALDRA LDREHFTSVV GADQDDAEML AEELRKFPAE
     TVLYLGALPE LSVSFLREQG FRTAWKDDPR EVHFLYANSY EREAAAQLFE KWLETHPMPQ
     ALFTTSFALL QGVMDVTLRR DGKLPSDLAI ATFGDNELLD FLQCPVLAVA QRHRDVAERV
     LEIVLASLDE PRKPKPGLTR IKRNLYRRGV LSRS