ID   CRA_SHIFL               Reviewed;         334 AA.
AC   P0ACP4; P21168;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=Catabolite repressor/activator;
DE   AltName: Full=Fructose repressor;
GN   Name=cra; Synonyms=fruR; OrderedLocusNames=SF0075, S0077;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Global transcriptional regulator, which plays an important
CC       role in the regulation of carbon metabolism. {ECO:0000250}.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
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DR   EMBL; AE005674; AAN41740.1; -; Genomic_DNA.
DR   EMBL; AE014073; AAP15621.1; -; Genomic_DNA.
DR   RefSeq; NP_706033.1; NC_004337.2.
DR   RefSeq; WP_000762401.1; NZ_UIQL01000009.1.
DR   AlphaFoldDB; P0ACP4; -.
DR   SMR; P0ACP4; -.
DR   STRING; 198214.SF0075; -.
DR   EnsemblBacteria; AAN41740; AAN41740; SF0075.
DR   EnsemblBacteria; AAP15621; AAP15621; S0077.
DR   GeneID; 1024540; -.
DR   GeneID; 67416153; -.
DR   KEGG; sfl:SF0075; -.
DR   KEGG; sfx:S0077; -.
DR   PATRIC; fig|198214.7.peg.88; -.
DR   HOGENOM; CLU_037628_6_0_6; -.
DR   OMA; NSRKAGY; -.
DR   OrthoDB; 1796334at2; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0009750; P:response to fructose; IEA:InterPro.
DR   CDD; cd01392; HTH_LacI; 1.
DR   Gene3D; 1.10.260.40; -; 1.
DR   InterPro; IPR012781; Fruct_sucro_rep.
DR   InterPro; IPR000843; HTH_LacI.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR001761; Peripla_BP/Lac1_sug-bd_dom.
DR   InterPro; IPR028082; Peripla_BP_I.
DR   Pfam; PF00356; LacI; 1.
DR   Pfam; PF00532; Peripla_BP_1; 1.
DR   SMART; SM00354; HTH_LACI; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
DR   SUPFAM; SSF53822; SSF53822; 1.
DR   TIGRFAMs; TIGR02417; fruct_sucro_rep; 1.
DR   PROSITE; PS00356; HTH_LACI_1; 1.
DR   PROSITE; PS50932; HTH_LACI_2; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..334
FT                   /note="Catabolite repressor/activator"
FT                   /id="PRO_0000107948"
FT   DOMAIN          1..58
FT                   /note="HTH lacI-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00111"
FT   DNA_BIND        3..22
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00111"
SQ   SEQUENCE   334 AA;  37999 MW;  503AE935CC2EC0C8 CRC64;
     MKLDEIARLA GVSRTTASYV INGKAKQYRV SDKTVEKVMA VVREHNYHPN AVAAGLRAGR
     TRSIGLVIPD LENTSYTRIA NYLERQARQR GYQLLIACSE DQPDNEMRCI EHLLQRQVDA
     IIVSTSLPPE HPFYQRWAND PFPIVALDRA LDREHFTSVV GADQDDAEML AEELRKFPAE
     TVLYLGALPE LSVSFLREQG FRTAWKDDPR EVHFLYANSY EREAAAQLFE KWLETHPMPQ
     ALFTTSFALL QGVMDVTLRR DGKLPSDLAI ATFGDNELLD FLQCPVLAVA QRHRDVAERV
     LEIVLASLDE PRKPKPGLTR IKRNLYRRGV LSRS