CRBG3_MOUSE
ID CRBG3_MOUSE Reviewed; 1005 AA.
AC Q80W49; Q3TRS6; Q8BMQ9;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Beta/gamma crystallin domain-containing protein 3;
GN Name=Crybg3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 619-1005 (ISOFORM 1/2/3).
RC STRAIN=C57BL/6J; TISSUE=Urinary bladder;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-122; SER-129; SER-130;
RP SER-136 AND SER-140, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE
RP ANALYSIS].
RC TISSUE=Heart, Kidney, Lung, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (1.86 ANGSTROMS) OF 510-601.
RX PubMed=23043265; DOI=10.1021/bi300844u;
RA Rajanikanth V., Srivastava S.S., Singh A.K., Rajyalakshmi M., Chandra K.,
RA Aravind P., Sankaranarayanan R., Sharma Y.;
RT "Aggregation-prone near-native intermediate formation during unfolding of a
RT structurally similar nonlenticular betagamma-crystallin domain.";
RL Biochemistry 51:8502-8513(2012).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q80W49-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q80W49-2; Sequence=VSP_032388;
CC Name=3;
CC IsoId=Q80W49-3; Sequence=VSP_032387, VSP_032389;
CC -!- SIMILARITY: Belongs to the beta/gamma-crystallin family. {ECO:0000305}.
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DR EMBL; AK030282; BAC26878.1; -; mRNA.
DR EMBL; AK162506; BAE36951.1; -; mRNA.
DR EMBL; AC154473; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC154854; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC043118; AAH43118.1; -; mRNA.
DR RefSeq; NP_777273.2; NM_174848.3.
DR PDB; 4FD9; X-ray; 1.86 A; A/B=510-601.
DR PDBsum; 4FD9; -.
DR AlphaFoldDB; Q80W49; -.
DR SMR; Q80W49; -.
DR STRING; 10090.ENSMUSP00000037682; -.
DR CAZy; CBM13; Carbohydrate-Binding Module Family 13.
DR iPTMnet; Q80W49; -.
DR PhosphoSitePlus; Q80W49; -.
DR EPD; Q80W49; -.
DR MaxQB; Q80W49; -.
DR PaxDb; Q80W49; -.
DR PeptideAtlas; Q80W49; -.
DR PRIDE; Q80W49; -.
DR ProteomicsDB; 285302; -. [Q80W49-1]
DR ProteomicsDB; 285303; -. [Q80W49-2]
DR ProteomicsDB; 285304; -. [Q80W49-3]
DR GeneID; 224273; -.
DR KEGG; mmu:224273; -.
DR UCSC; uc007zpj.2; mouse. [Q80W49-3]
DR UCSC; uc007zpk.2; mouse. [Q80W49-2]
DR CTD; 131544; -.
DR MGI; MGI:2676311; Crybg3.
DR eggNOG; ENOG502QTHR; Eukaryota.
DR HOGENOM; CLU_002147_1_0_1; -.
DR InParanoid; Q80W49; -.
DR OrthoDB; 89929at2759; -.
DR BioGRID-ORCS; 224273; 0 hits in 51 CRISPR screens.
DR ChiTaRS; Crybg3; mouse.
DR PRO; PR:Q80W49; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q80W49; protein.
DR Genevisible; Q80W49; MM.
DR GO; GO:0032991; C:protein-containing complex; ISO:MGI.
DR GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR GO; GO:0051018; F:protein kinase A binding; ISO:MGI.
DR GO; GO:0005212; F:structural constituent of eye lens; IBA:GO_Central.
DR GO; GO:0002088; P:lens development in camera-type eye; IBA:GO_Central.
DR GO; GO:0007601; P:visual perception; IBA:GO_Central.
DR CDD; cd00161; RICIN; 1.
DR InterPro; IPR001064; Beta/gamma_crystallin.
DR InterPro; IPR011024; G_crystallin-like.
DR InterPro; IPR035992; Ricin_B-like_lectins.
DR InterPro; IPR000772; Ricin_B_lectin.
DR Pfam; PF00030; Crystall; 6.
DR Pfam; PF00652; Ricin_B_lectin; 1.
DR PRINTS; PR01367; BGCRYSTALLIN.
DR SMART; SM00458; RICIN; 1.
DR SMART; SM00247; XTALbg; 5.
DR SUPFAM; SSF49695; SSF49695; 3.
DR SUPFAM; SSF50370; SSF50370; 1.
DR PROSITE; PS50915; CRYSTALLIN_BETA_GAMMA; 7.
DR PROSITE; PS50231; RICIN_B_LECTIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Lectin; Phosphoprotein;
KW Reference proteome; Repeat.
FT CHAIN 1..1005
FT /note="Beta/gamma crystallin domain-containing protein 3"
FT /id="PRO_0000325759"
FT DOMAIN 367..416
FT /note="Beta/gamma crystallin 'Greek key' 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT DOMAIN 462..500
FT /note="Beta/gamma crystallin 'Greek key' 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT DOMAIN 512..556
FT /note="Beta/gamma crystallin 'Greek key' 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT DOMAIN 557..599
FT /note="Beta/gamma crystallin 'Greek key' 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT DOMAIN 605..647
FT /note="Beta/gamma crystallin 'Greek key' 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT DOMAIN 648..690
FT /note="Beta/gamma crystallin 'Greek key' 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT DOMAIN 701..737
FT /note="Beta/gamma crystallin 'Greek key' 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT DOMAIN 738..781
FT /note="Beta/gamma crystallin 'Greek key' 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT DOMAIN 828..869
FT /note="Beta/gamma crystallin 'Greek key' 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT DOMAIN 871..1003
FT /note="Ricin B-type lectin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00174"
FT REGION 132..159
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 173..198
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 134..159
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 122
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 129
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 130
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 136
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 140
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 1..698
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_032387"
FT VAR_SEQ 1
FT /note="M -> MEVAMERTNGTTPGVTIMQTDALGPAFENTKDPREYMEKSIGEIEEP
FT PGEVKKGLILHDDRLASHFRGYESPTLSKDYEGYPASAIPDVQEEDTVVRLKKIM (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_032388"
FT VAR_SEQ 699..702
FT /note="PTHL -> MFSQ (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_032389"
FT STRAND 513..519
FT /evidence="ECO:0007829|PDB:4FD9"
FT HELIX 520..522
FT /evidence="ECO:0007829|PDB:4FD9"
FT STRAND 523..531
FT /evidence="ECO:0007829|PDB:4FD9"
FT HELIX 536..539
FT /evidence="ECO:0007829|PDB:4FD9"
FT STRAND 551..557
FT /evidence="ECO:0007829|PDB:4FD9"
FT STRAND 559..564
FT /evidence="ECO:0007829|PDB:4FD9"
FT TURN 565..567
FT /evidence="ECO:0007829|PDB:4FD9"
FT STRAND 568..574
FT /evidence="ECO:0007829|PDB:4FD9"
FT STRAND 576..579
FT /evidence="ECO:0007829|PDB:4FD9"
FT TURN 582..584
FT /evidence="ECO:0007829|PDB:4FD9"
FT HELIX 586..589
FT /evidence="ECO:0007829|PDB:4FD9"
FT STRAND 594..597
FT /evidence="ECO:0007829|PDB:4FD9"
SQ SEQUENCE 1005 AA; 114262 MW; 445C6ED46D2614A6 CRC64;
MSVPVVYDKR RNLDCREEKE ESNLAFVSQD EQDSSSFTIL YEEPLQEEDR YTSAELRGSQ
SLLFPDTSSM PGLACERSES RTDLVHHFEK EGKLGEAFDG DNSEMFLSVE AKRYKIYPLA
LSPIYEDDSS QEDVLSSEVS PGHHGSSKSR ESANQPSSVL SLLQSVSERL QRNFDGDDRQ
EAEEEEEEAV ASGKDWRTEK REHVTFHLPD PSIPFYPEDN QEHAGIFKSY VEFSEPTTSS
LQHGRWSEKE LFLQKSDMTS KLHSSLKSAY HQYLQTSRTH SSETGTRFGG TLQEPVSKYF
RVQDHAGRLS PYVENVDKQT LKCNPRPGKM IIYDLHGSKY KQEVYCNIPD ATTWSFPNGA
LIKVVRGCWI LYEKPHFQGQ KCVLEEGERV LDRDWLLQNR KHPERNFVLG SIKRVLKDCS
IPVIELCPKT DPGCSPIYIH RSVPNVEELN IPKSTSVTVK SGVWLAYPDI HFKGQATILE
EDQGLFEISA AEMKSLHPLQ MGGLKVEMPM NLKVILYEKP HFLGHTKEFS EHIDSVPTFL
KSDKDFHGIG SIRVIGGVWV AYEKEHFKGQ QFLLEEGDFE DSSACGALSG PIMSFRYLQA
NFIESSITLF ESSHLESGKF IDITNQEISD LEEIGFGSET RSIHVKSGVW VAYHQKFFCG
DQYILEKGKY KCFFDWGGSS NTILSIRPIQ LEPLGINEPT HLLKAFSKAG FQGECIDFVK
ECADLTSFTP ASFKVLRGCW LLLYYQEDGF YHQCVLEEGL YVDLTSCGCP SARIRALQPI
DYVFEEPSIS LFALEHCEGR ELHLEDAVNS VLNKDLHFYT QSVWIKSGLW IAYEGSNFLG
RQILLTPKEI PNWTAFSGWK TIGSVRPMKQ PAVYIRIRNR AQDEYLTVTG NPADARTMSV
CISPYSGKDT QIWHYCRGLF KSKASHTCLD VIGGRDTPGA KVALWTEHGQ LRQKWRMSRN
GTISSYLSDE LVLDVKGGNY YDKTHVIVNQ PLEGEETQKW DIEIL
