ID   2SS4_BRANA              Reviewed;         180 AA.
AC   P17333;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Napin;
DE   AltName: Full=1.7S seed storage protein;
DE   Contains:
DE     RecName: Full=Napin small chain;
DE   Contains:
DE     RecName: Full=Napin large chain;
DE   Flags: Precursor;
GN   Name=NAP1;
OS   Brassica napus (Rape).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=3708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Westar; TISSUE=Leaf;
RX   PubMed=2102844; DOI=10.1007/bf00027511;
RA   Baszczynski C.L., Fallis L.;
RT   "Isolation and nucleotide sequence of a genomic clone encoding a new
RT   Brassica napus napin gene.";
RL   Plant Mol. Biol. 14:633-635(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Topas;
RX   PubMed=7858212; DOI=10.1007/bf00019486;
RA   Boutilier K.A., Gines M.J., Demoor J.M., Huang B., Baszczynski C.L.,
RA   Iyer V.N., Miki B.L.A.;
RT   "Expression of the BnmNAP subfamily of napin genes coincides with the
RT   induction of Brassica microspore embryogenesis.";
RL   Plant Mol. Biol. 26:1711-1723(1994).
CC   -!- FUNCTION: The small, basic, water-soluble napins are one of the two
CC       major kinds of storage proteins synthesized in the seed during its
CC       maturation.
CC   -!- SUBUNIT: The mature protein consists of a small and a large chain
CC       linked by disulfide bonds.
CC   -!- TISSUE SPECIFICITY: Cotyledons and the axis.
CC   -!- SIMILARITY: Belongs to the 2S seed storage albumins family.
CC       {ECO:0000305}.
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DR   EMBL; X17542; CAA35580.1; -; Genomic_DNA.
DR   EMBL; U04945; AAA81909.1; -; mRNA.
DR   PIR; S10018; S10018.
DR   RefSeq; XP_013741649.1; XM_013886195.1.
DR   AlphaFoldDB; P17333; -.
DR   SMR; P17333; -.
DR   GeneID; 106444749; -.
DR   KEGG; bna:106444749; -.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR   CDD; cd00261; AAI_SS; 1.
DR   Gene3D; 1.10.110.10; -; 1.
DR   InterPro; IPR044723; AAI_SS_dom.
DR   InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR   InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR   InterPro; IPR000617; Napin/2SS/CON.
DR   PANTHER; PTHR35496; PTHR35496; 1.
DR   Pfam; PF00234; Tryp_alpha_amyl; 1.
DR   PRINTS; PR00496; NAPIN.
DR   SMART; SM00499; AAI; 1.
DR   SUPFAM; SSF47699; SSF47699; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Seed storage protein; Signal; Storage protein.
FT   SIGNAL          1..21
FT   PROPEP          22..38
FT                   /id="PRO_0000032119"
FT   CHAIN           39..74
FT                   /note="Napin small chain"
FT                   /id="PRO_0000032120"
FT   PROPEP          75..94
FT                   /id="PRO_0000032121"
FT   CHAIN           95..180
FT                   /note="Napin large chain"
FT                   /id="PRO_0000032122"
SQ   SEQUENCE   180 AA;  20318 MW;  6F3883CBED55FB26 CRC64;
     MANKLFLVSA TLAFFFLLTN ASIYRTIVEV DEDDATNPAG PFRIPKCRKE FQQAQHLKAC
     QQWLHKQAMQ SGSGPSWTLD GEFDFEDDME NPQGPQQRPP LLQQCCNELH QEEPLCVCPT
     LKGASKAVKQ QVRQQQGQQG QQLQQVISRI YQTATHLPKV CNIPQVSVCP FQKTMPGPSY