CRBN_DROPS
ID CRBN_DROPS Reviewed; 616 AA.
AC Q29B72;
DT 20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT 14-OCT-2008, sequence version 2.
DT 03-AUG-2022, entry version 60.
DE RecName: Full=Protein cereblon {ECO:0000250|UniProtKB:Q9VH36};
DE AltName: Full=Protein ohgata {ECO:0000250|UniProtKB:Q9VH36};
GN Name=ohgt {ECO:0000250|UniProtKB:Q9VH36};
GN Synonyms=crbn {ECO:0000250|UniProtKB:Q9VH36}; ORFNames=GA17779;
OS Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=46245;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MV2-25 / Tucson 14011-0121.94;
RX PubMed=15632085; DOI=10.1101/gr.3059305;
RA Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O.,
RA Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F.,
RA Howells S.L., Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A.,
RA Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M.,
RA Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J.,
RA Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y.,
RA Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA Weinstock G.M., Gibbs R.A.;
RT "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal,
RT gene, and cis-element evolution.";
RL Genome Res. 15:1-18(2005).
CC -!- FUNCTION: Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3
CC protein ligase complex that mediates the ubiquitination and subsequent
CC proteasomal degradation of target proteins. Has an essential role in
CC mediating growth by negatively regulating insulin signaling. It also
CC has a role in maintaining presynaptic function in the neuromuscular
CC junction synapses of third-instar larvae.
CC {ECO:0000250|UniProtKB:Q9VH36}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000250|UniProtKB:Q96SW2}.
CC -!- SUBUNIT: Likely a component of a DCX (DDB1-CUL4-X-box) protein ligase
CC complex (By similarity). May interact with pic/DDB1 (By similarity).
CC {ECO:0000250|UniProtKB:Q96SW2, ECO:0000250|UniProtKB:Q9VH36}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9VH36}.
CC -!- PTM: Ubiquitinated. {ECO:0000250|UniProtKB:Q9VH36}.
CC -!- SIMILARITY: Belongs to the CRBN family. {ECO:0000305}.
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DR EMBL; CM000070; EAL27126.2; -; Genomic_DNA.
DR RefSeq; XP_001357990.2; XM_001357953.3.
DR AlphaFoldDB; Q29B72; -.
DR SMR; Q29B72; -.
DR STRING; 7237.FBpp0282652; -.
DR EnsemblMetazoa; FBtr0284214; FBpp0282652; FBgn0077788.
DR GeneID; 4800775; -.
DR KEGG; dpo:Dpse_GA17779; -.
DR eggNOG; KOG1400; Eukaryota.
DR HOGENOM; CLU_028769_0_0_1; -.
DR InParanoid; Q29B72; -.
DR OMA; AYQMYDS; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000001819; Chromosome 2.
DR Bgee; FBgn0077788; Expressed in male reproductive system and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:1900075; P:positive regulation of neuromuscular synaptic transmission; IEA:EnsemblMetazoa.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd15777; CRBN_C_like; 1.
DR InterPro; IPR034750; CULT.
DR InterPro; IPR003111; Lon_prtase_N.
DR InterPro; IPR004910; Yippee/Mis18/Cereblon.
DR Pfam; PF03226; Yippee-Mis18; 1.
DR PROSITE; PS51788; CULT; 1.
DR PROSITE; PS51787; LON_N; 1.
PE 3: Inferred from homology;
KW Metal-binding; Nucleus; Reference proteome; Ubl conjugation;
KW Ubl conjugation pathway; Zinc.
FT CHAIN 1..616
FT /note="Protein cereblon"
FT /id="PRO_0000393884"
FT DOMAIN 257..480
FT /note="Lon N-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01123"
FT DOMAIN 481..590
FT /note="CULT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01124"
FT REGION 1..39
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 63..137
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 182..220
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 486
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01124"
FT BINDING 489
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01124"
FT BINDING 555
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01124"
FT BINDING 558
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01124"
SQ SEQUENCE 616 AA; 69131 MW; 8EEE97ED0AD2738F CRC64;
MDEEETAEIN AQEQEVAGSA GEAAAGPSGA EVQPNDDSVA VERVIDVFEE TAEMEAAMIE
RFFGPSGEVA EEGTLPVPGP SADGEGSANA GGERPSEEDI VLDDGTESDG SYNRPGSDMS
LDSPNSEDDS DVEAMPRWMI PQNRLRFAVD MMVSQARNQD GGIAALLNRD NFLQRVRSMV
FSQERRRSRN SDEVSPEAED DELPEHPPPP PPRPPIDIDM EEGVHFDTNL PAEHSYFGPN
LNRVPGVNYQ EVGSTHHMLI FLHQYILFPG EVLPFMIDGS LFDDDMSGLD GLIFAVAFPL
MKPPEDSKKL YGVTCQIYEK GDNGRHLTFY KSRALQRIVI NCSDIKGLPQ YIARNPTNKC
HSKVKILPEY FLPEPLKCID MGSMSRFRDI PSMRDKYLRY QISSTPWPVE VCQEYAYEDI
VERARKKLEV HKIDTMPKCP IQMSFWLVRN LHLTEKMMSQ MFLTDSVNLR LQLIGGILKE
ETLFYCRYCN SSLAYCSDLF AMSKHGVQTQ YCNPGGYIHE TNTVYRVISH AIGYSGEPST
RFSWFPGYQW HIILCKFCAQ HVGWEFKAVE PNLAPKMFYG LAGSSVRIGK AGDSATVNGN
NFVVRNMLRV ISEGME
