ID   CRCB2_METHJ             Reviewed;         120 AA.
AC   Q2FLB7;
DT   17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Putative fluoride ion transporter CrcB 2 {ECO:0000255|HAMAP-Rule:MF_00454};
GN   Name=crcB2 {ECO:0000255|HAMAP-Rule:MF_00454}; OrderedLocusNames=Mhun_1099;
OS   Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 /
OS   JF-1).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanomicrobiales; Methanospirillaceae; Methanospirillum.
OX   NCBI_TaxID=323259;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27890 / DSM 864 / NBRC 100397 / JF-1;
RX   PubMed=26744606; DOI=10.1186/s40793-015-0124-8;
RA   Gunsalus R.P., Cook L.E., Crable B., Rohlin L., McDonald E., Mouttaki H.,
RA   Sieber J.R., Poweleit N., Zhou H., Lapidus A.L., Daligault H.E., Land M.,
RA   Gilna P., Ivanova N., Kyrpides N., Culley D.E., McInerney M.J.;
RT   "Complete genome sequence of Methanospirillum hungatei type strain JF1.";
RL   Stand. Genomic Sci. 11:2-2(2016).
CC   -!- FUNCTION: Important for reducing fluoride concentration in the cell,
CC       thus reducing its toxicity. {ECO:0000255|HAMAP-Rule:MF_00454}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_00454};
CC       Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_00454}.
CC   -!- SIMILARITY: Belongs to the CrcB (TC 9.B.71) family. {ECO:0000255|HAMAP-
CC       Rule:MF_00454}.
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DR   EMBL; CP000254; ABD40848.1; -; Genomic_DNA.
DR   RefSeq; WP_011448126.1; NC_007796.1.
DR   AlphaFoldDB; Q2FLB7; -.
DR   SMR; Q2FLB7; -.
DR   STRING; 323259.Mhun_1099; -.
DR   EnsemblBacteria; ABD40848; ABD40848; Mhun_1099.
DR   GeneID; 3922132; -.
DR   KEGG; mhu:Mhun_1099; -.
DR   eggNOG; arCOG04701; Archaea.
DR   HOGENOM; CLU_114342_1_2_2; -.
DR   OMA; TGICGSF; -.
DR   OrthoDB; 112898at2157; -.
DR   Proteomes; UP000001941; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:1903425; F:fluoride transmembrane transporter activity; IEA:UniProt.
DR   HAMAP; MF_00454; CrcB; 1.
DR   InterPro; IPR003691; CrcB.
DR   PANTHER; PTHR28259; PTHR28259; 1.
DR   Pfam; PF02537; CRCB; 1.
DR   TIGRFAMs; TIGR00494; crcB; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..120
FT                   /note="Putative fluoride ion transporter CrcB 2"
FT                   /id="PRO_0000252966"
FT   TRANSMEM        30..50
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00454"
FT   TRANSMEM        57..77
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00454"
FT   TRANSMEM        97..117
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00454"
SQ   SEQUENCE   120 AA;  12952 MW;  5B1A87621643D5D1 CRC64;
     MEHLILVGIG GAVGAMLRFE LSKLRPVRSI PLGTALVNII GSSLFGCVVF SRSPGDIFYL
     VDVGILGGFT TFSTFSFETF RMFEEQDYQT MVLNISINLI GSLIGVLLSF ILVTLILTGG