CRCB_ECOLI
ID CRCB_ECOLI Reviewed; 127 AA.
AC P37002;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Putative fluoride ion transporter CrcB {ECO:0000255|HAMAP-Rule:MF_00454};
GN Name=crcB {ECO:0000255|HAMAP-Rule:MF_00454}; Synonyms=ybeI;
GN OrderedLocusNames=b0624, JW0619;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RA Yamanaka K., Mitani T., Ogura T., Niki H., Hiraga S.;
RL Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND OVEREXPRESSION.
RC STRAIN=K12;
RX PubMed=8844142; DOI=10.1093/genetics/143.4.1521;
RA Hu K.H., Liu E., Dean K., Gingras M., Degraff W., Trun N.J.;
RT "Overproduction of three genes leads to camphor resistance and chromosome
RT condensation in Escherichia coli.";
RL Genetics 143:1521-1532(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [7]
RP IDENTIFICATION.
RA Rudd K.E.;
RL Unpublished observations (MAR-1994).
RN [8]
RP DISRUPTION PHENOTYPE, AND OVEREXPRESSION.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=12904550; DOI=10.1099/mic.0.26363-0;
RA Sand O., Gingras M., Beck N., Hall C., Trun N.;
RT "Phenotypic characterization of overexpression or deletion of the
RT Escherichia coli crcA, cspE and crcB genes.";
RL Microbiology 149:2107-2117(2003).
RN [9]
RP TOPOLOGY [LARGE SCALE ANALYSIS], AND SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [10]
RP TOPOLOGY, AND SUBCELLULAR LOCATION.
RX PubMed=16429150; DOI=10.1038/nsmb1057;
RA Rapp M., Granseth E., Seppala S., von Heijne G.;
RT "Identification and evolution of dual-topology membrane proteins.";
RL Nat. Struct. Mol. Biol. 13:112-116(2006).
RN [11]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22194412; DOI=10.1126/science.1215063;
RA Baker J.L., Sudarsan N., Weinberg Z., Roth A., Stockbridge R.B.,
RA Breaker R.R.;
RT "Widespread genetic switches and toxicity resistance proteins for
RT fluoride.";
RL Science 335:233-235(2012).
CC -!- FUNCTION: Important for reducing fluoride concentration in the cell,
CC thus reducing its toxicity. {ECO:0000255|HAMAP-Rule:MF_00454,
CC ECO:0000269|PubMed:22194412}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_00454, ECO:0000269|PubMed:15919996,
CC ECO:0000269|PubMed:16429150}; Multi-pass membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_00454, ECO:0000269|PubMed:15919996,
CC ECO:0000269|PubMed:16429150}. Note=Could be a dual-topology protein,
CC which inserts into the membrane in two opposite orientations. May form
CC an antiparallel homodimer in the inner membrane.
CC -!- INDUCTION: By fluoride, via a fluoride-responsive riboswitch.
CC {ECO:0000269|PubMed:22194412}.
CC -!- DISRUPTION PHENOTYPE: Mutants show increased sensitivity to fluoride
CC (PubMed:22194412). Deletion of pagP (crcA), cspE and crcB increases
CC sensitivity to camphor and exacerbates the nucleoid morphology defects
CC of the topoisomerase IV temperature-sensitive mutants
CC (PubMed:12904550). {ECO:0000269|PubMed:12904550,
CC ECO:0000269|PubMed:22194412}.
CC -!- MISCELLANEOUS: Overexpression of pagP (crcA), cspE and crcB leads to
CC camphor resistance, chromosome condensation and suppression of growth
CC defects of mukB mutants (PubMed:8844142). Overexpression also increases
CC levels of plasmid supercoiling, results in increased resistance to
CC nalidixic acid in gyrase and topoisomerase IV temperature-sensitive
CC mutants and affects the regulation of several genes (PubMed:12904550).
CC {ECO:0000305|PubMed:12904550, ECO:0000305|PubMed:8844142}.
CC -!- SIMILARITY: Belongs to the CrcB (TC 9.B.71) family. {ECO:0000255|HAMAP-
CC Rule:MF_00454}.
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DR EMBL; D28497; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; S83396; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; U82598; AAB40824.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73725.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35267.1; -; Genomic_DNA.
DR PIR; F64796; F64796.
DR RefSeq; NP_415157.1; NC_000913.3.
DR RefSeq; WP_000939747.1; NZ_SSZK01000037.1.
DR AlphaFoldDB; P37002; -.
DR SMR; P37002; -.
DR BioGRID; 4260641; 196.
DR IntAct; P37002; 1.
DR STRING; 511145.b0624; -.
DR TCDB; 1.A.43.1.1; the camphor resistance or fluoride exporter (fluc) family.
DR PaxDb; P37002; -.
DR PRIDE; P37002; -.
DR EnsemblBacteria; AAC73725; AAC73725; b0624.
DR EnsemblBacteria; BAA35267; BAA35267; BAA35267.
DR GeneID; 945798; -.
DR KEGG; ecj:JW0619; -.
DR KEGG; eco:b0624; -.
DR PATRIC; fig|511145.12.peg.654; -.
DR EchoBASE; EB2123; -.
DR eggNOG; COG0239; Bacteria.
DR HOGENOM; CLU_114342_3_3_6; -.
DR InParanoid; P37002; -.
DR OMA; FLMFESF; -.
DR PhylomeDB; P37002; -.
DR BioCyc; EcoCyc:EG12209-MON; -.
DR BioCyc; MetaCyc:EG12209-MON; -.
DR PRO; PR:P37002; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0005253; F:anion channel activity; IDA:EcoCyc.
DR GO; GO:1903425; F:fluoride transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0140114; P:cellular detoxification of fluoride; IMP:EcoCyc.
DR GO; GO:1903424; P:fluoride transmembrane transport; IDA:EcoCyc.
DR HAMAP; MF_00454; CrcB; 1.
DR InterPro; IPR003691; CrcB.
DR PANTHER; PTHR28259; PTHR28259; 1.
DR Pfam; PF02537; CRCB; 1.
DR TIGRFAMs; TIGR00494; crcB; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..127
FT /note="Putative fluoride ion transporter CrcB"
FT /id="PRO_0000110097"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00454"
FT TRANSMEM 35..55
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00454"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00454"
FT TRANSMEM 103..123
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00454"
SQ SEQUENCE 127 AA; 13777 MW; 5A0B2004D134BBF3 CRC64;
MLQLLLAVFI GGGTGSVARW LLSMRFNPLH QAIPLGTLTA NLIGAFIIGI GFAWFSRMTN
IDPVWKVLIT TGFCGGLTTF STFSAEVVFL LQEGRFGWAL LNVFVNLLGS FAMTALAFWL
FSASTAH
