ID   2SS8_HELAN              Reviewed;         141 AA.
AC   P23110;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Albumin-8;
DE   AltName: Full=Methionine-rich 2S protein;
DE   AltName: Full=SFA8;
DE   Flags: Precursor;
OS   Helianthus annuus (Common sunflower).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Asterales; Asteraceae; Asteroideae;
OC   Heliantheae alliance; Heliantheae; Helianthus.
OX   NCBI_TaxID=4232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 39-141.
RC   TISSUE=Seed;
RX   PubMed=1997318; DOI=10.1111/j.1432-1033.1991.tb15710.x;
RA   Kortt A.A., Caldwell J.B., Lilley G.G., Higgins T.J.V.;
RT   "Amino acid and cDNA sequences of a methionine-rich 2S protein from
RT   sunflower seed (Helianthus annuus L.).";
RL   Eur. J. Biochem. 195:329-334(1991).
RN   [2]
RP   PROTEIN SEQUENCE OF 39-141.
RC   STRAIN=cv. Hybrid 246; TISSUE=Seed;
RX   PubMed=8915004; DOI=10.1016/0014-5793(96)01117-9;
RA   Egorov T.A., Odintsova T.I., Musolyamov A.K., Fido R., Tatham A.S.,
RA   Shewry P.R.;
RT   "Disulphide structure of a sunflower seed albumin: conserved and variant
RT   disulphide bonds in the cereal prolamin superfamily.";
RL   FEBS Lett. 396:285-288(1996).
RN   [3]
RP   STRUCTURE BY NMR OF 39-141, AND DISULFIDE BONDS.
RX   PubMed=15170335; DOI=10.1021/bi0496900;
RA   Pantoja-Uceda D., Shewry P.R., Bruix M., Tatham A.S., Santoro J., Rico M.;
RT   "Solution structure of a methionine-rich 2S albumin from sunflower seeds:
RT   relationship to its allergenic and emulsifying properties.";
RL   Biochemistry 43:6976-6986(2004).
CC   -!- FUNCTION: This is a 2S seed storage protein.
CC   -!- SUBUNIT: Heterodimer; disulfide-linked. {ECO:0000269|PubMed:15170335}.
CC   -!- SIMILARITY: Belongs to the 2S seed storage albumins family.
CC       {ECO:0000305}.
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DR   EMBL; X56686; CAA40015.1; -; mRNA.
DR   PIR; S14259; S14259.
DR   PDB; 1S6D; NMR; -; A=39-141.
DR   PDBsum; 1S6D; -.
DR   AlphaFoldDB; P23110; -.
DR   SMR; P23110; -.
DR   Allergome; 772; Hel a 2S Albumin.
DR   EvolutionaryTrace; P23110; -.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR   CDD; cd00261; AAI_SS; 1.
DR   Gene3D; 1.10.110.10; -; 1.
DR   InterPro; IPR044723; AAI_SS_dom.
DR   InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR   InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR   InterPro; IPR000617; Napin/2SS/CON.
DR   PANTHER; PTHR35496; PTHR35496; 1.
DR   Pfam; PF00234; Tryp_alpha_amyl; 1.
DR   SMART; SM00499; AAI; 1.
DR   SUPFAM; SSF47699; SSF47699; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Disulfide bond;
KW   Seed storage protein; Signal; Storage protein.
FT   SIGNAL          1..25
FT   PROPEP          26..38
FT                   /evidence="ECO:0000269|PubMed:1997318,
FT                   ECO:0000269|PubMed:8915004"
FT                   /id="PRO_0000032153"
FT   CHAIN           39..141
FT                   /note="Albumin-8"
FT                   /id="PRO_0000032154"
FT   DISULFID        49..100
FT                   /evidence="ECO:0000269|PubMed:15170335"
FT   DISULFID        62..89
FT                   /evidence="ECO:0000269|PubMed:15170335"
FT   DISULFID        90..132
FT                   /evidence="ECO:0000269|PubMed:15170335"
FT   DISULFID        102..139
FT                   /evidence="ECO:0000269|PubMed:15170335"
FT   CONFLICT        67
FT                   /note="M -> N (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   STRAND          46..48
FT                   /evidence="ECO:0007829|PDB:1S6D"
FT   HELIX           49..55
FT                   /evidence="ECO:0007829|PDB:1S6D"
FT   HELIX           61..66
FT                   /evidence="ECO:0007829|PDB:1S6D"
FT   TURN            67..70
FT                   /evidence="ECO:0007829|PDB:1S6D"
FT   STRAND          77..79
FT                   /evidence="ECO:0007829|PDB:1S6D"
FT   HELIX           87..95
FT                   /evidence="ECO:0007829|PDB:1S6D"
FT   HELIX           98..100
FT                   /evidence="ECO:0007829|PDB:1S6D"
FT   HELIX           103..108
FT                   /evidence="ECO:0007829|PDB:1S6D"
FT   HELIX           118..131
FT                   /evidence="ECO:0007829|PDB:1S6D"
FT   STRAND          134..137
FT                   /evidence="ECO:0007829|PDB:1S6D"
SQ   SEQUENCE   141 AA;  16090 MW;  1E5723B9122C9BD4 CRC64;
     MARFSIVFAA AGVLLLVAMA PVSEASTTTI ITTIIEENPY GRGRTESGCY QQMEEAEMLN
     HCGMYLMKNL GERSQVSPRM REEDHKQLCC MQLKNLDEKC MCPAIMMMLN EPMWIRMRDQ
     VMSMAHNLPI ECNLMSQPCQ M