ID   2SSB_BRANA              Reviewed;         178 AA.
AC   P27740;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Napin-B;
DE   AltName: Full=1.7S seed storage protein;
DE   Contains:
DE     RecName: Full=Napin-B small chain;
DE   Contains:
DE     RecName: Full=Napin-B large chain;
DE   Flags: Precursor;
GN   Name=NAPB;
OS   Brassica napus (Rape).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=3708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Svalofs Karat 20516-K;
RX   PubMed=2029903; DOI=10.1111/j.1432-1033.1991.tb15966.x;
RA   Ericson M.L., Muren E., Gustavsson H.O., Josefsson L.G., Rask L.;
RT   "Analysis of the promoter region of napin genes from Brassica napus
RT   demonstrates binding of nuclear protein in vitro to a conserved sequence
RT   motif.";
RL   Eur. J. Biochem. 197:741-746(1991).
CC   -!- FUNCTION: The small, basic, water-soluble napins are one of the two
CC       major kinds of storage proteins synthesized in the seed during its
CC       maturation.
CC   -!- SUBUNIT: The mature protein consists of a small and a large chain
CC       linked by disulfide bonds.
CC   -!- TISSUE SPECIFICITY: Cotyledons and the axis.
CC   -!- SIMILARITY: Belongs to the 2S seed storage albumins family.
CC       {ECO:0000305}.
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DR   EMBL; X58142; CAA41150.1; -; Genomic_DNA.
DR   PIR; S15382; S15382.
DR   AlphaFoldDB; P27740; -.
DR   SMR; P27740; -.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR   CDD; cd00261; AAI_SS; 1.
DR   Gene3D; 1.10.110.10; -; 1.
DR   InterPro; IPR044723; AAI_SS_dom.
DR   InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR   InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR   InterPro; IPR000617; Napin/2SS/CON.
DR   PANTHER; PTHR35496; PTHR35496; 1.
DR   Pfam; PF00234; Tryp_alpha_amyl; 1.
DR   PRINTS; PR00496; NAPIN.
DR   SMART; SM00499; AAI; 1.
DR   SUPFAM; SSF47699; SSF47699; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Seed storage protein; Signal; Storage protein.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000250"
FT   PROPEP          22..38
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000032123"
FT   CHAIN           39..74
FT                   /note="Napin-B small chain"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000032124"
FT   PROPEP          75..94
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000032125"
FT   CHAIN           95..178
FT                   /note="Napin-B large chain"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000032126"
SQ   SEQUENCE   178 AA;  20114 MW;  96CE0ADB7CD966E9 CRC64;
     MANKLFLVSA TLAFFFLLTN ASIYRTVVEF DEDDATNPAG PFRIPKCRKE FQQAQHLKAC
     QQWLHKQAMQ SGSGPSWTLD GEFDFEDDME NPQGPQQRPP LLQQCCNELH QEEPLCVCPT
     LKGASKAVKQ QIQQQGQQQG KLQMVSRIYQ TATHLPKVCK IPQVSVCPFQ KTMPGPSY