CRCH_SYNWW
ID CRCH_SYNWW Reviewed; 260 AA.
AC Q0AVM1;
DT 25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Crotonyl-CoA hydratase {ECO:0000303|PubMed:23468890};
DE EC=4.2.1.150 {ECO:0000305|PubMed:23468890};
GN OrderedLocusNames=Swol_1936 {ECO:0000312|EMBL:ABI69233.1};
OS Syntrophomonas wolfei subsp. wolfei (strain DSM 2245B / Goettingen).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Syntrophomonadaceae;
OC Syntrophomonas.
OX NCBI_TaxID=335541;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 2245B / Goettingen;
RX PubMed=21966920; DOI=10.1111/j.1462-2920.2010.02237.x;
RA Sieber J.R., Sims D.R., Han C., Kim E., Lykidis A., Lapidus A.L.,
RA McDonnald E., Rohlin L., Culley D.E., Gunsalus R., McInerney M.J.;
RT "The genome of Syntrophomonas wolfei: new insights into syntrophic
RT metabolism and biohydrogen production.";
RL Environ. Microbiol. 12:2289-2301(2010).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION, SUBCELLULAR LOCATION,
RP FUNCTION, AND PATHWAY.
RX PubMed=23468890; DOI=10.1371/journal.pone.0056905;
RA Schmidt A., Mueller N., Schink B., Schleheck D.;
RT "A proteomic view at the biochemistry of syntrophic butyrate oxidation in
RT Syntrophomonas wolfei.";
RL PLoS ONE 8:E56905-E56905(2013).
CC -!- FUNCTION: Involved in syntrophic growth of S.wolfei with butyrate, as
CC part of the butyrate oxidation pathway. Probably catalyzes the
CC hydration of crotonyl-CoA to 3-hydroxybutyryl-CoA.
CC {ECO:0000305|PubMed:23468890}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-hydroxybutanoyl-CoA = (2E)-butenoyl-CoA + H2O;
CC Xref=Rhea:RHEA:45584, ChEBI:CHEBI:15377, ChEBI:CHEBI:57332,
CC ChEBI:CHEBI:78611; Evidence={ECO:0000305|PubMed:23468890};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a short-chain (3S)-3-hydroxyacyl-CoA = a short-chain (2E)-
CC enoyl-CoA + H2O; Xref=Rhea:RHEA:52664, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:87488, ChEBI:CHEBI:136760; EC=4.2.1.150;
CC Evidence={ECO:0000305|PubMed:23468890};
CC -!- PATHWAY: Lipid metabolism; butanoate metabolism.
CC {ECO:0000305|PubMed:23468890}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000250|UniProtKB:P52046}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:23468890}.
CC -!- INDUCTION: Highly expressed during syntrophic growth with butyrate (at
CC protein level). Seems to be constitutively expressed.
CC {ECO:0000269|PubMed:23468890}.
CC -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC {ECO:0000305}.
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DR EMBL; CP000448; ABI69233.1; -; Genomic_DNA.
DR RefSeq; WP_011641326.1; NC_008346.1.
DR AlphaFoldDB; Q0AVM1; -.
DR SMR; Q0AVM1; -.
DR STRING; 335541.Swol_1936; -.
DR PRIDE; Q0AVM1; -.
DR EnsemblBacteria; ABI69233; ABI69233; Swol_1936.
DR KEGG; swo:Swol_1936; -.
DR eggNOG; COG1024; Bacteria.
DR HOGENOM; CLU_009834_7_6_9; -.
DR OMA; GLRFERH; -.
DR OrthoDB; 1498685at2; -.
DR UniPathway; UPA00863; -.
DR Proteomes; UP000001968; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0120092; F:crotonyl-CoA hydratase activity; IEA:RHEA.
DR GO; GO:0019605; P:butyrate metabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.12.10; -; 1.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS.
DR InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR InterPro; IPR014748; Enoyl-CoA_hydra_C.
DR Pfam; PF00378; ECH_1; 1.
DR SUPFAM; SSF52096; SSF52096; 1.
DR PROSITE; PS00166; ENOYL_COA_HYDRATASE; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Fatty acid metabolism; Lipid metabolism; Lyase;
KW Reference proteome.
FT CHAIN 1..260
FT /note="Crotonyl-CoA hydratase"
FT /id="PRO_0000442215"
FT ACT_SITE 114
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:Q5LLW6"
FT ACT_SITE 134
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q5LLW6"
SQ SEQUENCE 260 AA; 27944 MW; A52D1637D982807E CRC64;
MAYENIILEK EEKLAVLYIN RPKAMNALNK DTLLEIKDAV TAVNDDPAVE LLIITGSGDK
SFVAGADIAF MQNLSAMEAR EFGALGQKVF RLIEAMEKPV IAAVNGFALG GGCELAMCCD
FRIAASNAKF GQPEVGLGIT PGFGGTQRLP RLVGPGMAKQ LLYTADVINA DEAFRIGLVN
KVVQPEELLP EVKKIAGRIL SKGQLAVRLS KAAANEGMQT DIDRAMSIEA DAFGLCFATQ
DQKEGMTAFL EKRKANFISK