CRC_ARATH
ID CRC_ARATH Reviewed; 181 AA.
AC Q8L925; Q9LQA3; Q9XHD1;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 2.
DT 25-MAY-2022, entry version 119.
DE RecName: Full=Protein CRABS CLAW {ECO:0000303|PubMed:10225998};
GN Name=CRC {ECO:0000303|PubMed:10225998};
GN OrderedLocusNames=At1g69180 {ECO:0000312|Araport:AT1G69180};
GN ORFNames=F23O10.23 {ECO:0000312|EMBL:AAG52485.1},
GN F4N2.14 {ECO:0000312|EMBL:AAF27068.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, TISSUE
RP SPECIFICITY, INDUCTION, AND MUTAGENESIS OF GLU-129.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=10225998; DOI=10.1242/dev.126.11.2387;
RA Bowman J.L., Smyth D.R.;
RT "CRABS CLAW, a gene that regulates carpel and nectary development in
RT Arabidopsis, encodes a novel protein with zinc finger and helix-loop-helix
RT domains.";
RL Development 126:2387-2396(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION.
RX PubMed=10535738; DOI=10.1016/s0092-8674(00)81651-7;
RA Eshed Y., Baum S.F., Bowman J.L.;
RT "Distinct mechanisms promote polarity establishment in carpels of
RT Arabidopsis.";
RL Cell 99:199-209(1999).
RN [7]
RP FUNCTION.
RX PubMed=10225997; DOI=10.1242/dev.126.11.2377;
RA Alvarez J., Smyth D.R.;
RT "CRABS CLAW and SPATULA, two Arabidopsis genes that control carpel
RT development in parallel with AGAMOUS.";
RL Development 126:2377-2386(1999).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=10679447; DOI=10.1016/s1369-5266(99)00035-7;
RA Bowman J.L.;
RT "The YABBY gene family and abaxial cell fate.";
RL Curr. Opin. Plant Biol. 3:17-22(2000).
RN [9]
RP FUNCTION.
RX PubMed=11714690; DOI=10.1242/dev.128.22.4657;
RA Baum S.F., Eshed Y., Bowman J.L.;
RT "The Arabidopsis nectary is an ABC-independent floral structure.";
RL Development 128:4657-4667(2001).
RN [10]
RP FUNCTION.
RX AGRICOLA=IND23265209; DOI=10.1086/324178;
RA Alvarez J., Smyth D.R.;
RT "CRABS CLAW and SPATULA genes regulate growth and pattern formation during
RT gynoecium development in Arabidopsis thaliana.";
RL Int. J. Plant Sci. 163:17-41(2002).
RN [11]
RP FUNCTION, AND INDUCTION.
RX PubMed=15598802; DOI=10.1105/tpc.104.026666;
RA Lee J.-Y., Baum S.F., Alvarez J., Patel A., Chitwood D.H., Bowman J.L.;
RT "Activation of CRABS CLAW in the nectaries and carpels of Arabidopsis.";
RL Plant Cell 17:25-36(2005).
RN [12]
RP DISRUPTION PHENOTYPE.
RX PubMed=18441215; DOI=10.1105/tpc.107.053306;
RA Prunet N., Morel P., Thierry A.-M., Eshed Y., Bowman J.L., Negrutiu I.,
RA Trehin C.;
RT "REBELOTE, SQUINT, and ULTRAPETALA1 function redundantly in the temporal
RT regulation of floral meristem termination in Arabidopsis thaliana.";
RL Plant Cell 20:901-919(2008).
CC -!- FUNCTION: Transcription factor required for the initiation of nectary
CC development. Also involved in suppressing early radial growth of the
CC gynoecium, in promoting its later elongation and in fusion of its
CC carpels by regulating both cell division and expansion. Establishes the
CC polar differentiation in the carpels by specifying abaxial cell fate in
CC the ovary wall. Regulates both cell division and expansion.
CC {ECO:0000269|PubMed:10225997, ECO:0000269|PubMed:10225998,
CC ECO:0000269|PubMed:10535738, ECO:0000269|PubMed:11714690,
CC ECO:0000269|PubMed:15598802, ECO:0000269|Ref.10}.
CC -!- INTERACTION:
CC Q8L925; Q9LEZ3: BIM1; NbExp=3; IntAct=EBI-15197527, EBI-617095;
CC Q8L925; F4IPE3: SGR5; NbExp=3; IntAct=EBI-15197527, EBI-15192881;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Restricted to flowers, mostly in carpels and
CC nectaries. Expressed at low levels in sepal primordia (buds), sepal
CC receptacle and developing petal. Not detected in placental tissues,
CC septum, stigma and ovules. {ECO:0000269|PubMed:10225998}.
CC -!- DEVELOPMENTAL STAGE: In carpels, expression starts when the gynoecial
CC primordia becomes distinct. First expressed in the lateral region of
CC each carpel. Later resolves into two distinct domains, epidermal and
CC internal. In epidermal tissues, mostly localized on the outer surface,
CC successively from the base to the tip of the gynoecium and finally
CC confined to the valve region before disappearing during last flowering
CC stages. In internal tissues, first confined to four discrete zones
CC adjacent to the future placental tissue occupying the full length of
CC the elongating cylinder, declining from the apical regions and
CC disappearing after the ovule primordia arise. Before nectaries
CC initiation, expression occupies a ring of receptacle cells between the
CC stamen and sepal primordia, from where nectaries will develop. Strongly
CC expressed in nectaries until latest flowering stages.
CC {ECO:0000269|PubMed:10225998}.
CC -!- INDUCTION: Down-regulated by SPT and by A class genes AP2 and LUG in
CC the outer whorl. In the third whorl, B class genes AP3 and PI, and the
CC C class gene AG act redundantly with each other and in combination with
CC SEP1, SEP2, SEP3, SHP1 and SHP2 to activate CRC in nectaries and
CC carpels. LFY enhances its expression. {ECO:0000269|PubMed:10225998,
CC ECO:0000269|PubMed:15598802}.
CC -!- DISRUPTION PHENOTYPE: The double mutants crc-1 rbl-1, crc-1 sqn-4, and
CC crc-1 ult1-4 exhibit strong floral meristem (FM) indeterminacy with
CC reiterations of extra floral whorls in the center of the flower.
CC {ECO:0000269|PubMed:18441215}.
CC -!- SIMILARITY: Belongs to the YABBY family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF27068.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAM66994.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF132606; AAD30526.1; -; mRNA.
DR EMBL; AC008262; AAF27068.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC018364; AAG52485.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34891.1; -; Genomic_DNA.
DR EMBL; BT008618; AAP40440.1; -; mRNA.
DR EMBL; AY088672; AAM66994.1; ALT_INIT; mRNA.
DR PIR; G96715; G96715.
DR RefSeq; NP_177078.1; NM_105585.3.
DR AlphaFoldDB; Q8L925; -.
DR SMR; Q8L925; -.
DR BioGRID; 28470; 14.
DR IntAct; Q8L925; 14.
DR STRING; 3702.AT1G69180.1; -.
DR iPTMnet; Q8L925; -.
DR PaxDb; Q8L925; -.
DR PRIDE; Q8L925; -.
DR ProteomicsDB; 222637; -.
DR EnsemblPlants; AT1G69180.1; AT1G69180.1; AT1G69180.
DR GeneID; 843249; -.
DR Gramene; AT1G69180.1; AT1G69180.1; AT1G69180.
DR KEGG; ath:AT1G69180; -.
DR Araport; AT1G69180; -.
DR TAIR; locus:2026418; AT1G69180.
DR eggNOG; ENOG502QVCI; Eukaryota.
DR HOGENOM; CLU_071156_2_0_1; -.
DR OMA; MNHEEKV; -.
DR OrthoDB; 1325348at2759; -.
DR PhylomeDB; Q8L925; -.
DR PRO; PR:Q8L925; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8L925; baseline and differential.
DR Genevisible; Q8L925; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043565; F:sequence-specific DNA binding; IPI:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0048440; P:carpel development; IMP:TAIR.
DR GO; GO:0045165; P:cell fate commitment; IBA:GO_Central.
DR GO; GO:0010582; P:floral meristem determinacy; IGI:TAIR.
DR GO; GO:0048366; P:leaf development; IBA:GO_Central.
DR GO; GO:0010254; P:nectary development; IMP:TAIR.
DR GO; GO:0009944; P:polarity specification of adaxial/abaxial axis; TAS:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR GO; GO:0048479; P:style development; IMP:TAIR.
DR Gene3D; 1.10.30.10; -; 1.
DR InterPro; IPR036910; HMG_box_dom_sf.
DR InterPro; IPR006780; YABBY.
DR PANTHER; PTHR31675; PTHR31675; 1.
DR Pfam; PF04690; YABBY; 1.
DR SUPFAM; SSF47095; SSF47095; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; DNA-binding; Flowering;
KW Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..181
FT /note="Protein CRABS CLAW"
FT /id="PRO_0000133716"
FT ZN_FING 26..53
FT /note="C4-type"
FT /evidence="ECO:0000255"
FT REGION 80..122
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 80..98
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 129
FT /note="E->K: In crc-7; abnormal gynoecium and lack of
FT nectaries."
FT /evidence="ECO:0000269|PubMed:10225998"
FT CONFLICT 155
FT /note="K -> T (in Ref. 5; AAM66994)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 181 AA; 19722 MW; 67A4A78B48A92B4A CRC64;
MNLEEKPTMT ASRASPQAEH LYYVRCSICN TILAVGIPLK RMLDTVTVKC GHCGNLSFLT
TTPPLQGHVS LTLQMQSFGG SDYKKGSSSS SSSSTSSDQP PSPSPPFVVK PPEKKQRLPS
AYNRFMRDEI QRIKSANPEI PHREAFSAAA KNWAKYIPNS PTSITSGGHN MIHGLGFGEK
K
