ID   CRD1_EUPES              Reviewed;         405 AA.
AC   Q945B7;
DT   16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase, chloroplastic;
DE            Short=Mg-protoporphyrin IX monomethyl ester oxidative cyclase;
DE            EC=1.14.13.81;
DE   Flags: Precursor;
GN   Name=CRD1; Synonyms=AT103;
OS   Euphorbia esula (Leafy spurge).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Malpighiales; Euphorbiaceae; Euphorbioideae; Euphorbieae;
OC   Euphorbia; Euphorbia subgen. Esula; Euphorbia sect. Esula.
OX   NCBI_TaxID=3993;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Horvath D.P.;
RT   "Novel regulation and promoter analysis of a gene with similarity to the
RT   leucine zipper-containing gene At103.";
RL   Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the formation of the isocyclic ring in chlorophyll
CC       biosynthesis. Mediates the cyclase reaction, which results in the
CC       formation of divinylprotochlorophyllide (Pchlide) characteristic of all
CC       chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester
CC       (MgPMME) (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + Mg-protoporphyrin IX 13-monomethyl ester + 3 NADPH +
CC         3 O2 = 3,8-divinyl protochlorophyllide a + 5 H2O + 3 NADP(+);
CC         Xref=Rhea:RHEA:33235, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC         ChEBI:CHEBI:58632, ChEBI:CHEBI:60491; EC=1.14.13.81;
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000250};
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC       biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the AcsF family. {ECO:0000305}.
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DR   EMBL; AF417577; AAL13304.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q945B7; -.
DR   UniPathway; UPA00668; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0048529; F:magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   CDD; cd01047; ACSF; 1.
DR   HAMAP; MF_01840; AcsF; 1.
DR   InterPro; IPR008434; AcsF.
DR   InterPro; IPR009078; Ferritin-like_SF.
DR   InterPro; IPR003251; Rubrerythrin.
DR   PANTHER; PTHR31053; PTHR31053; 1.
DR   Pfam; PF02915; Rubrerythrin; 1.
DR   SUPFAM; SSF47240; SSF47240; 1.
DR   TIGRFAMs; TIGR02029; AcsF; 1.
PE   3: Inferred from homology;
KW   Chlorophyll biosynthesis; Chloroplast; Iron; Metal-binding; NADP;
KW   Oxidoreductase; Photosynthesis; Plastid; Transit peptide.
FT   TRANSIT         1..44
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           45..405
FT                   /note="Magnesium-protoporphyrin IX monomethyl ester
FT                   [oxidative] cyclase, chloroplastic"
FT                   /id="PRO_0000000601"
FT   REGION          33..54
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        33..48
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   405 AA;  47310 MW;  A0011A0E4B58372C CRC64;
     MAAEMALVKP ITPKFINPMR TFSSSSKFST IKMSATSQSN TTTTATKPSK KGNKKEINET
     LLTPRFYTTD FDEMETLFNT EINKKLNQSE FEALLQEFKT DYNQTHFVRN KEFKEAADKM
     QGPLRQIFVE FLERSCTAEF SGFLLYKELG RRLKKTNPVV AEIFSLMSRD EARHAGFLNK
     GLSDFNYALD LGFLTKARKY TFFKPKFIFY ATYLSEKIGY WRYITIYRHL KENPEYQCYP
     IFKYFENWCQ DENRHGDFFS ALMKAQPQFL NDWKAKLWAR FFCLSVYVTM YLNDCQRTAF
     YEGIGLDTKE FDMHVIIETN RTTARIFPAV LDVENPEFKR KLDRMVVINQ KLQAVGETED
     NSVVKNLKRV PLIAALVSEI LAAYLMPPIE SGSVDFAEFE PKLVY