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CRD1_HORVU
ID   CRD1_HORVU              Reviewed;         417 AA.
AC   Q5EFU4;
DT   16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase, chloroplastic;
DE            Short=Mg-protoporphyrin IX monomethyl ester oxidative cyclase;
DE            EC=1.14.13.81;
DE   AltName: Full=Protein Xantha-l;
DE   Flags: Precursor;
GN   Name=CRD1;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], ENZYME ACTIVITY, PATHWAY, FUNCTION,
RP   SUBCELLULAR LOCATION, AND MUTAGENESIS OF GLY-154 AND SER-180.
RX   PubMed=15824317; DOI=10.1073/pnas.0501784102;
RA   Rzeznicka K., Walker C.J., Westergren T., Kannangara C.G.,
RA   von Wettstein D., Merchant S., Gough S.P., Hansson M.;
RT   "Xantha-l encodes a membrane subunit of the aerobic Mg-protoporphyrin IX
RT   monomethyl ester cyclase involved in chlorophyll biosynthesis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:5886-5891(2005).
CC   -!- FUNCTION: Catalyzes the formation of the isocyclic ring in chlorophyll
CC       biosynthesis. Mediates the cyclase reaction, which results in the
CC       formation of divinylprotochlorophyllide (Pchlide) characteristic of all
CC       chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester
CC       (MgPMME). {ECO:0000269|PubMed:15824317}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + Mg-protoporphyrin IX 13-monomethyl ester + 3 NADPH +
CC         3 O2 = 3,8-divinyl protochlorophyllide a + 5 H2O + 3 NADP(+);
CC         Xref=Rhea:RHEA:33235, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC         ChEBI:CHEBI:58632, ChEBI:CHEBI:60491; EC=1.14.13.81;
CC         Evidence={ECO:0000269|PubMed:15824317};
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000250};
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC       biosynthesis. {ECO:0000269|PubMed:15824317}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC       Peripheral membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the AcsF family. {ECO:0000305}.
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DR   EMBL; AY887063; AAW80518.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q5EFU4; -.
DR   EnsemblPlants; HORVU.MOREX.r2.3HG0214090.1; HORVU.MOREX.r2.3HG0214090.1; HORVU.MOREX.r2.3HG0214090.
DR   EnsemblPlants; HORVU.MOREX.r2.3HG0214090.1.mrna1; HORVU.MOREX.r2.3HG0214090.1.mrna1; HORVU.MOREX.r2.3HG0214090.1.
DR   Gramene; HORVU.MOREX.r2.3HG0214090.1; HORVU.MOREX.r2.3HG0214090.1; HORVU.MOREX.r2.3HG0214090.
DR   Gramene; HORVU.MOREX.r2.3HG0214090.1.mrna1; HORVU.MOREX.r2.3HG0214090.1.mrna1; HORVU.MOREX.r2.3HG0214090.1.
DR   BRENDA; 1.14.13.81; 2687.
DR   UniPathway; UPA00668; -.
DR   ExpressionAtlas; Q5EFU4; baseline and differential.
DR   GO; GO:0009706; C:chloroplast inner membrane; IEA:EnsemblPlants.
DR   GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:EnsemblPlants.
DR   GO; GO:0048529; F:magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0003729; F:mRNA binding; IEA:EnsemblPlants.
DR   GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0009658; P:chloroplast organization; IEA:EnsemblPlants.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   GO; GO:1901401; P:regulation of tetrapyrrole metabolic process; IEA:EnsemblPlants.
DR   CDD; cd01047; ACSF; 1.
DR   HAMAP; MF_01840; AcsF; 1.
DR   InterPro; IPR008434; AcsF.
DR   InterPro; IPR009078; Ferritin-like_SF.
DR   InterPro; IPR003251; Rubrerythrin.
DR   PANTHER; PTHR31053; PTHR31053; 1.
DR   Pfam; PF02915; Rubrerythrin; 1.
DR   SUPFAM; SSF47240; SSF47240; 1.
DR   TIGRFAMs; TIGR02029; AcsF; 1.
PE   1: Evidence at protein level;
KW   Chlorophyll biosynthesis; Chloroplast; Iron; Membrane; Metal-binding; NADP;
KW   Oxidoreductase; Photosynthesis; Plastid; Transit peptide.
FT   TRANSIT         1..45
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           46..417
FT                   /note="Magnesium-protoporphyrin IX monomethyl ester
FT                   [oxidative] cyclase, chloroplastic"
FT                   /id="PRO_0000000603"
FT   MUTAGEN         154
FT                   /note="G->E: In xantha-l(81); induces accumulation of
FT                   MgPMME."
FT                   /evidence="ECO:0000269|PubMed:15824317"
FT   MUTAGEN         180
FT                   /note="S->F: In xantha-l(35); induces accumulation of
FT                   MgPMME."
FT                   /evidence="ECO:0000269|PubMed:15824317"
SQ   SEQUENCE   417 AA;  48161 MW;  83933398C325E77E CRC64;
     MASAMELSLL NPAMHHYGIA AKTASHLPVV PARRASSGAV RFRVRAAAAA PPAPAAKPGS
     PKKRGKTEVN ESLLTPRFYT TDFDEMEQLF NAEINKQLNQ DEFDALLQEF KTDYNQTHFI
     RNPEFKEAAD KMQGPLRQIF VEFLERSCTA EFSGFLLYKE LGRRLKKTNP VVAEIFSLMS
     RDEARHAGFL NKGLSDFNLA LDLGFLTKAR KYTFFKPKFI FYATYLSEKI GYWRYITIFR
     HLKANPEYQV YPIFKYFENW CQDENRHGDF FSALLKAQPQ FLNDWKAKLW SRFFCLSVYI
     TMYLNDCQRS AFYEGIGLNT KEFDMHVIYE TNRTTARIFP AVPDVENPEF KRKLDRMVDI
     NLKIISIGES NDLPLVKNLK RVPLIAQLVS EIIAAYLMPP IESGSVDFAE FEPKLVY
 
 
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