CRD1_HORVU
ID CRD1_HORVU Reviewed; 417 AA.
AC Q5EFU4;
DT 16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 25-MAY-2022, entry version 83.
DE RecName: Full=Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase, chloroplastic;
DE Short=Mg-protoporphyrin IX monomethyl ester oxidative cyclase;
DE EC=1.14.13.81;
DE AltName: Full=Protein Xantha-l;
DE Flags: Precursor;
GN Name=CRD1;
OS Hordeum vulgare (Barley).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX NCBI_TaxID=4513;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], ENZYME ACTIVITY, PATHWAY, FUNCTION,
RP SUBCELLULAR LOCATION, AND MUTAGENESIS OF GLY-154 AND SER-180.
RX PubMed=15824317; DOI=10.1073/pnas.0501784102;
RA Rzeznicka K., Walker C.J., Westergren T., Kannangara C.G.,
RA von Wettstein D., Merchant S., Gough S.P., Hansson M.;
RT "Xantha-l encodes a membrane subunit of the aerobic Mg-protoporphyrin IX
RT monomethyl ester cyclase involved in chlorophyll biosynthesis.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:5886-5891(2005).
CC -!- FUNCTION: Catalyzes the formation of the isocyclic ring in chlorophyll
CC biosynthesis. Mediates the cyclase reaction, which results in the
CC formation of divinylprotochlorophyllide (Pchlide) characteristic of all
CC chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester
CC (MgPMME). {ECO:0000269|PubMed:15824317}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+) + Mg-protoporphyrin IX 13-monomethyl ester + 3 NADPH +
CC 3 O2 = 3,8-divinyl protochlorophyllide a + 5 H2O + 3 NADP(+);
CC Xref=Rhea:RHEA:33235, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC ChEBI:CHEBI:58632, ChEBI:CHEBI:60491; EC=1.14.13.81;
CC Evidence={ECO:0000269|PubMed:15824317};
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000250};
CC -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC biosynthesis. {ECO:0000269|PubMed:15824317}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the AcsF family. {ECO:0000305}.
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DR EMBL; AY887063; AAW80518.1; -; Genomic_DNA.
DR AlphaFoldDB; Q5EFU4; -.
DR EnsemblPlants; HORVU.MOREX.r2.3HG0214090.1; HORVU.MOREX.r2.3HG0214090.1; HORVU.MOREX.r2.3HG0214090.
DR EnsemblPlants; HORVU.MOREX.r2.3HG0214090.1.mrna1; HORVU.MOREX.r2.3HG0214090.1.mrna1; HORVU.MOREX.r2.3HG0214090.1.
DR Gramene; HORVU.MOREX.r2.3HG0214090.1; HORVU.MOREX.r2.3HG0214090.1; HORVU.MOREX.r2.3HG0214090.
DR Gramene; HORVU.MOREX.r2.3HG0214090.1.mrna1; HORVU.MOREX.r2.3HG0214090.1.mrna1; HORVU.MOREX.r2.3HG0214090.1.
DR BRENDA; 1.14.13.81; 2687.
DR UniPathway; UPA00668; -.
DR ExpressionAtlas; Q5EFU4; baseline and differential.
DR GO; GO:0009706; C:chloroplast inner membrane; IEA:EnsemblPlants.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:EnsemblPlants.
DR GO; GO:0048529; F:magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003729; F:mRNA binding; IEA:EnsemblPlants.
DR GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0009658; P:chloroplast organization; IEA:EnsemblPlants.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR GO; GO:1901401; P:regulation of tetrapyrrole metabolic process; IEA:EnsemblPlants.
DR CDD; cd01047; ACSF; 1.
DR HAMAP; MF_01840; AcsF; 1.
DR InterPro; IPR008434; AcsF.
DR InterPro; IPR009078; Ferritin-like_SF.
DR InterPro; IPR003251; Rubrerythrin.
DR PANTHER; PTHR31053; PTHR31053; 1.
DR Pfam; PF02915; Rubrerythrin; 1.
DR SUPFAM; SSF47240; SSF47240; 1.
DR TIGRFAMs; TIGR02029; AcsF; 1.
PE 1: Evidence at protein level;
KW Chlorophyll biosynthesis; Chloroplast; Iron; Membrane; Metal-binding; NADP;
KW Oxidoreductase; Photosynthesis; Plastid; Transit peptide.
FT TRANSIT 1..45
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 46..417
FT /note="Magnesium-protoporphyrin IX monomethyl ester
FT [oxidative] cyclase, chloroplastic"
FT /id="PRO_0000000603"
FT MUTAGEN 154
FT /note="G->E: In xantha-l(81); induces accumulation of
FT MgPMME."
FT /evidence="ECO:0000269|PubMed:15824317"
FT MUTAGEN 180
FT /note="S->F: In xantha-l(35); induces accumulation of
FT MgPMME."
FT /evidence="ECO:0000269|PubMed:15824317"
SQ SEQUENCE 417 AA; 48161 MW; 83933398C325E77E CRC64;
MASAMELSLL NPAMHHYGIA AKTASHLPVV PARRASSGAV RFRVRAAAAA PPAPAAKPGS
PKKRGKTEVN ESLLTPRFYT TDFDEMEQLF NAEINKQLNQ DEFDALLQEF KTDYNQTHFI
RNPEFKEAAD KMQGPLRQIF VEFLERSCTA EFSGFLLYKE LGRRLKKTNP VVAEIFSLMS
RDEARHAGFL NKGLSDFNLA LDLGFLTKAR KYTFFKPKFI FYATYLSEKI GYWRYITIFR
HLKANPEYQV YPIFKYFENW CQDENRHGDF FSALLKAQPQ FLNDWKAKLW SRFFCLSVYI
TMYLNDCQRS AFYEGIGLNT KEFDMHVIYE TNRTTARIFP AVPDVENPEF KRKLDRMVDI
NLKIISIGES NDLPLVKNLK RVPLIAQLVS EIIAAYLMPP IESGSVDFAE FEPKLVY