CRDR_HELPY
ID CRDR_HELPY Reviewed; 213 AA.
AC O25918;
DT 11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 141.
DE RecName: Full=Transcriptional regulatory protein CrdR {ECO:0000303|PubMed:15968080};
GN Name=crdR {ECO:0000303|PubMed:15968080}; OrderedLocusNames=HP_1365;
OS Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=85962;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700392 / 26695;
RX PubMed=9252185; DOI=10.1038/41483;
RA Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT "The complete genome sequence of the gastric pathogen Helicobacter
RT pylori.";
RL Nature 388:539-547(1997).
RN [2]
RP DISRUPTION PHENOTYPE.
RX PubMed=12933888; DOI=10.1128/iai.71.9.5381-5385.2003;
RA Panthel K., Dietz P., Haas R., Beier D.;
RT "Two-component systems of Helicobacter pylori contribute to virulence in a
RT mouse infection model.";
RL Infect. Immun. 71:5381-5385(2003).
RN [3]
RP FUNCTION.
RX PubMed=15968080; DOI=10.1128/jb.187.13.4683-4688.2005;
RA Waidner B., Melchers K., Staehler F.N., Kist M., Bereswill S.;
RT "The Helicobacter pylori CrdRS two-component regulation system
RT (HP1364/HP1365) is required for copper-mediated induction of the copper
RT resistance determinant CrdA.";
RL J. Bacteriol. 187:4683-4688(2005).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=26082024; DOI=10.1111/mmi.13089;
RA Hung C.L., Cheng H.H., Hsieh W.C., Tsai Z.T., Tsai H.K., Chu C.H.,
RA Hsieh W.P., Chen Y.F., Tsou Y., Lai C.H., Wang W.C.;
RT "The CrdRS two-component system in Helicobacter pylori responds to
RT nitrosative stress.";
RL Mol. Microbiol. 97:1128-1141(2015).
CC -!- FUNCTION: Member of the two-component regulatory system CrdR/CrdS that
CC induces the transcriptional induction of the copper resistance
CC determinant CrdA (PubMed:12933888). Upon phosphorylation by CrdS,
CC functions as a transcriptional regulator by direct binding to promoter
CC regions of target genes including the crdA promoter or nitric oxide-
CC responsive gene promoters (PubMed:26082024).
CC {ECO:0000269|PubMed:12933888, ECO:0000269|PubMed:26082024}.
CC -!- PTM: Phosphorylated by CrdS. {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Deletion mutant is not able to colonize the
CC stomach in mice (PubMed:12933888). Shows also a significant loss of
CC viability upon exposure to nitric oxide (PubMed:26082024).
CC {ECO:0000269|PubMed:12933888, ECO:0000269|PubMed:26082024}.
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DR EMBL; AE000511; AAD08405.1; -; Genomic_DNA.
DR PIR; E64690; E64690.
DR RefSeq; NP_208157.1; NC_000915.1.
DR RefSeq; WP_001169789.1; NC_018939.1.
DR AlphaFoldDB; O25918; -.
DR SMR; O25918; -.
DR IntAct; O25918; 1.
DR STRING; 85962.C694_07045; -.
DR PaxDb; O25918; -.
DR EnsemblBacteria; AAD08405; AAD08405; HP_1365.
DR KEGG; hpy:HP_1365; -.
DR PATRIC; fig|85962.47.peg.1462; -.
DR eggNOG; COG0745; Bacteria.
DR OMA; RTMDTHV; -.
DR PhylomeDB; O25918; -.
DR Proteomes; UP000000429; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0032993; C:protein-DNA complex; IBA:GO_Central.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IBA:GO_Central.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR CDD; cd00383; trans_reg_C; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR039420; WalR-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR48111; PTHR48111; 1.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF00486; Trans_reg_C; 1.
DR SMART; SM00448; REC; 1.
DR SMART; SM00862; Trans_reg_C; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS51755; OMPR_PHOB; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 3: Inferred from homology;
KW DNA-binding; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation; Two-component regulatory system.
FT CHAIN 1..213
FT /note="Transcriptional regulatory protein CrdR"
FT /id="PRO_0000448705"
FT DOMAIN 4..119
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DNA_BIND 121..212
FT /note="OmpR/PhoB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT MOD_RES 53
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ SEQUENCE 213 AA; 24872 MW; 17F7543923017900 CRC64;
MQKKIFLLED DYLLSESVKE FLEHLGYEVF CAFNGKEAYE RLSVERFNLL LLDVQVPEMN
SLELFKRIKN DFLISTPVIF ITALQDNATL KNAFNLGASD YLKKPFDLDE LEARIKRFFN
DDPIEIMPNI FYHQNCLSVR GKKEILPPKT AQLLEYFLEH KGQIISSQAL ENNLWEQAID
DSTLRTYIKV LRKLLGKNCI ETHKGVGYRF NPL
