ID   CRE15_PHYIT             Reviewed;         349 AA.
AC   D0NVS7;
DT   08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2009, sequence version 1.
DT   03-AUG-2022, entry version 30.
DE   RecName: Full=RxLR effector protein CRE15 {ECO:0000303|PubMed:29312401};
DE   AltName: Full=Core RXLR effector 15 {ECO:0000303|PubMed:29312401};
DE   Flags: Precursor;
GN   Name=CRE15 {ECO:0000303|PubMed:29312401}; ORFNames=PITG_17316;
OS   Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=403677;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND INDUCTION.
RC   STRAIN=T30-4;
RX   PubMed=19741609; DOI=10.1038/nature08358;
RG   The Broad Institute Genome Sequencing Platform;
RA   Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA   Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA   Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA   Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA   Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA   Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA   Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA   Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA   Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA   Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA   Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA   Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA   Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA   Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA   Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA   Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA   Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA   Nusbaum C.;
RT   "Genome sequence and analysis of the Irish potato famine pathogen
RT   Phytophthora infestans.";
RL   Nature 461:393-398(2009).
RN   [2]
RP   INDUCTION.
RX   PubMed=23055926; DOI=10.1371/journal.ppat.1002940;
RA   Cooke D.E., Cano L.M., Raffaele S., Bain R.A., Cooke L.R.,
RA   Etherington G.J., Deahl K.L., Farrer R.A., Gilroy E.M., Goss E.M.,
RA   Gruenwald N.J., Hein I., MacLean D., McNicol J.W., Randall E., Oliva R.F.,
RA   Pel M.A., Shaw D.S., Squires J.N., Taylor M.C., Vleeshouwers V.G.,
RA   Birch P.R., Lees A.K., Kamoun S.;
RT   "Genome analyses of an aggressive and invasive lineage of the Irish potato
RT   famine pathogen.";
RL   PLoS Pathog. 8:E1002940-E1002940(2012).
RN   [3]
RP   INDUCTION.
RX   PubMed=28228125; DOI=10.1186/s12864-017-3585-x;
RA   Ah-Fong A.M., Kim K.S., Judelson H.S.;
RT   "RNA-seq of life stages of the oomycete Phytophthora infestans reveals
RT   dynamic changes in metabolic, signal transduction, and pathogenesis genes
RT   and a major role for calcium signaling in development.";
RL   BMC Genomics 18:198-198(2017).
RN   [4]
RP   INDUCTION.
RX   PubMed=29312401; DOI=10.3389/fpls.2017.02155;
RA   Yin J., Gu B., Huang G., Tian Y., Quan J., Lindqvist-Kreuze H., Shan W.;
RT   "Conserved RXLR effector genes of Phytophthora infestans expressed at the
RT   early stage of potato infection are suppressive to host defense.";
RL   Front. Plant Sci. 8:2155-2155(2017).
RN   [5]
RP   FUNCTION, INTERACTION WITH HOST VIK, AND SUBCELLULAR LOCATION.
RX   PubMed=29588335; DOI=10.1104/pp.18.00028;
RA   Murphy F., He Q., Armstrong M., Giuliani L.M., Boevink P.C., Zhang W.,
RA   Tian Z., Birch P.R.J., Gilroy E.M.;
RT   "The potato MAP3K StVIK is required for the Phytophthora infestans RXLR
RT   effector Pi17316 to promote disease.";
RL   Plant Physiol. 177:398-410(2018).
CC   -!- FUNCTION: Effector that promotes P.infestans virulence in Nicotiana
CC       benthamiana and potato (PubMed:29588335). Attenuates cell death
CC       triggered by the pathogen-associated molecular pattern infestin 1
CC       (INF1), indicating that the effector suppresses pattern-triggered
CC       immunity. However, it does not attenuate cell death triggered by a
CC       range of resistance proteins, suggesting that it specifically
CC       suppresses INF1-triggered cell death (ICD) (PubMed:29588335). Targets
CC       host MAP3K VIK in order to utilize or promote its ability to negatively
CC       regulate immunity (PubMed:29588335). {ECO:0000269|PubMed:29588335}.
CC   -!- SUBUNIT: Interacts directly with the potato ortholog of vascular
CC       highway 1 (VH1)-interacting kinase (VIK), encoding a predicted MEK
CC       kinase (MAP3K). {ECO:0000269|PubMed:29588335}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:29588335}. Host cell
CC       membrane {ECO:0000269|PubMed:29588335}.
CC   -!- INDUCTION: Expression is up-regulated during the biotrophic phase of
CC       infection on host plants. {ECO:0000269|PubMed:19741609,
CC       ECO:0000269|PubMed:23055926, ECO:0000269|PubMed:28228125,
CC       ECO:0000269|PubMed:29312401}.
CC   -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC       cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC       phosphate. {ECO:0000305|PubMed:29588335}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; DS028170; EEY66758.1; -; Genomic_DNA.
DR   RefSeq; XP_002896823.1; XM_002896777.1.
DR   AlphaFoldDB; D0NVS7; -.
DR   SMR; D0NVS7; -.
DR   EnsemblProtists; PITG_17316T0; PITG_17316T0; PITG_17316.
DR   GeneID; 9466553; -.
DR   KEGG; pif:PITG_17316; -.
DR   VEuPathDB; FungiDB:PITG_17316; -.
DR   eggNOG; ENOG502RG36; Eukaryota.
DR   HOGENOM; CLU_021192_4_1_1; -.
DR   Proteomes; UP000006643; Partially assembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Host cell membrane; Host membrane; Membrane; Reference proteome; Secreted;
KW   Signal.
FT   SIGNAL          1..33
FT                   /evidence="ECO:0000255"
FT   CHAIN           34..349
FT                   /note="RxLR effector protein CRE15"
FT                   /id="PRO_5003013116"
FT   MOTIF           46..62
FT                   /note="RxLR-dEER"
FT                   /evidence="ECO:0000305|PubMed:29588335"
SQ   SEQUENCE   349 AA;  39588 MW;  E53E92AEB57E406F CRC64;
     MITFKRLSSA RWGALLTSIA VLFFLAITKG ADAKAGADLA DIQAYRRLRT TTADAYYASE
     DRVFSVVKAL KDLAHNAKLT FSLDKQLKVN NRFEVLRVKQ VKTDVFSSSE FTDWAHYVAK
     ICKRGRLPAD RAIFKTMAAH YGDDELARML ATAKRTSRDT VVYQLKEIQQ KSWKESGKSA
     DDVYAILQLD AGGQNVLNNP GLPAWLSYVK SPSTDYIEAL LLKLREQYDD VTVAKMIVSS
     QSGVNKRISG QLEKELSTAW RKNHITEMEV FQLLKLNDAG TTLLKNPILE IWFHYVWKMK
     RNDPYELLVS WFKKAGIDDA GLGKMIATAK QDDRNYWIAQ TLEQRLSGK