CRE4_PHYIT
ID CRE4_PHYIT Reviewed; 198 AA.
AC D0N607;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2009, sequence version 1.
DT 03-AUG-2022, entry version 31.
DE RecName: Full=RxLR effector protein CRE4 {ECO:0000303|PubMed:30329083};
DE AltName: Full=Core RXLR effector 4 {ECO:0000303|PubMed:29312401};
DE Flags: Precursor;
GN Name=CRE4; ORFNames=PITG_05910;
OS Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=403677;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=T30-4;
RX PubMed=19741609; DOI=10.1038/nature08358;
RG The Broad Institute Genome Sequencing Platform;
RA Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA Nusbaum C.;
RT "Genome sequence and analysis of the Irish potato famine pathogen
RT Phytophthora infestans.";
RL Nature 461:393-398(2009).
RN [2]
RP INDUCTION.
RX PubMed=28228125; DOI=10.1186/s12864-017-3585-x;
RA Ah-Fong A.M., Kim K.S., Judelson H.S.;
RT "RNA-seq of life stages of the oomycete Phytophthora infestans reveals
RT dynamic changes in metabolic, signal transduction, and pathogenesis genes
RT and a major role for calcium signaling in development.";
RL BMC Genomics 18:198-198(2017).
RN [3]
RP INDUCTION, FUNCTION, AND DOMAIN.
RX PubMed=29312401; DOI=10.3389/fpls.2017.02155;
RA Yin J., Gu B., Huang G., Tian Y., Quan J., Lindqvist-Kreuze H., Shan W.;
RT "Conserved RXLR effector genes of Phytophthora infestans expressed at the
RT early stage of potato infection are suppressive to host defense.";
RL Front. Plant Sci. 8:2155-2155(2017).
RN [4]
RP SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=30329083; DOI=10.1093/jxb/ery360;
RA Wang S., McLellan H., Bukharova T., He Q., Murphy F., Shi J., Sun S.,
RA van Weymers P., Ren Y., Thilliez G., Wang H., Chen X., Engelhardt S.,
RA Vleeshouwers V., Gilroy E.M., Whisson S.C., Hein I., Wang X., Tian Z.,
RA Birch P.R.J., Boevink P.C.;
RT "Phytophthora infestans RXLR effectors act in concert at diverse
RT subcellular locations to enhance host colonization.";
RL J. Exp. Bot. 70:343-356(2019).
CC -!- FUNCTION: Effector that is involved in host plant infection.
CC Contributes to virulence during the early infection stage, by
CC inhibiting plant defense responses induced by both PAMP-triggered
CC immunity (PTI) and effector-triggered immunity (ETI).
CC {ECO:0000269|PubMed:29312401, ECO:0000269|PubMed:30329083}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30329083}. Host
CC cytoplasm {ECO:0000269|PubMed:30329083}. Host nucleus, host nucleolus
CC {ECO:0000269|PubMed:30329083}. Host nucleus
CC {ECO:0000269|PubMed:30329083}.
CC -!- INDUCTION: Expression is induced during host plant infection.
CC {ECO:0000269|PubMed:28228125, ECO:0000269|PubMed:29312401}.
CC -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC phosphate. {ECO:0000305|PubMed:29312401}.
CC -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR EMBL; DS028126; EEY70498.1; -; Genomic_DNA.
DR RefSeq; XP_002998152.1; XM_002998106.1.
DR AlphaFoldDB; D0N607; -.
DR SMR; D0N607; -.
DR EnsemblProtists; PITG_05910T0; PITG_05910T0; PITG_05910.
DR GeneID; 9472119; -.
DR KEGG; pif:PITG_05910; -.
DR VEuPathDB; FungiDB:PITG_05910; -.
DR eggNOG; ENOG502RGQG; Eukaryota.
DR HOGENOM; CLU_122622_0_0_1; -.
DR InParanoid; D0N607; -.
DR OMA; THETRFV; -.
DR OrthoDB; 1628730at2759; -.
DR Proteomes; UP000006643; Partially assembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell.
PE 2: Evidence at transcript level;
KW Host cytoplasm; Host nucleus; Reference proteome; Secreted; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..198
FT /note="RxLR effector protein CRE4"
FT /id="PRO_5003011722"
FT MOTIF 43..52
FT /note="RxLR-dEER"
FT /evidence="ECO:0000305|PubMed:29312401"
SQ SEQUENCE 198 AA; 22448 MW; 418CEB0D790EC553 CRC64;
MLRSFLLIVA TVSLFGQCKP LPLATSPVSD AVRAPHRSTH ETRFVRTNDE ERGATMTLAG
VLRDKAQTKQ LLTSWLNSGK SVPSVSNKLG LKRMSLEQAI HHENWKALTT FQRMKSKKAK
AYAKYGTGYQ TEAKTKENLL QWVMRGDSPK EVSSTLGLLG LSRRKIIDHQ NYEAFRTFLK
YRKQWAEMQG NGFTKLTT
