ID   CREA_BACB0              Reviewed;         411 AA.
AC   P38487;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Creatinase;
DE            EC=3.5.3.3;
DE   AltName: Full=Creatine amidinohydrolase;
OS   Bacillus sp. (strain B-0618).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=69000;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-21.
RA   Suzuki K., Sagai H., Sugiyama M., Imamura S.;
RT   "Molecular cloning and high expression of the Bacillus creatinase gene in
RT   Escherichia coli.";
RL   J. Ferment. Bioeng. 76:77-81(1993).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=creatine + H2O = sarcosine + urea; Xref=Rhea:RHEA:22456,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:16199, ChEBI:CHEBI:57433,
CC         ChEBI:CHEBI:57947; EC=3.5.3.3;
CC   -!- SUBUNIT: Homodimer.
CC   -!- INDUCTION: By choline chloride.
CC   -!- SIMILARITY: Belongs to the peptidase M24 family. Creatinase subfamily.
CC       {ECO:0000305}.
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DR   EMBL; D14463; BAA03358.1; -; Genomic_DNA.
DR   PIR; I39809; I39809.
DR   AlphaFoldDB; P38487; -.
DR   SMR; P38487; -.
DR   GO; GO:0016980; F:creatinase activity; IEA:UniProtKB-EC.
DR   CDD; cd01090; Creatinase; 1.
DR   Gene3D; 3.40.350.10; -; 1.
DR   Gene3D; 3.90.230.10; -; 1.
DR   InterPro; IPR029149; Creatin/AminoP/Spt16_NTD.
DR   InterPro; IPR036005; Creatinase/aminopeptidase-like.
DR   InterPro; IPR039394; Creatinase_C.
DR   InterPro; IPR000587; Creatinase_N.
DR   InterPro; IPR000994; Pept_M24.
DR   Pfam; PF01321; Creatinase_N; 1.
DR   Pfam; PF00557; Peptidase_M24; 1.
DR   SUPFAM; SSF53092; SSF53092; 1.
DR   SUPFAM; SSF55920; SSF55920; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|Ref.1"
FT   CHAIN           2..411
FT                   /note="Creatinase"
FT                   /id="PRO_0000079338"
FT   ACT_SITE        240
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   411 AA;  46949 MW;  66620F1501CDADC9 CRC64;
     MQQITDLERT KILQNGGEKV KPTFSKEEMT RRNTRLREYM AKAGIDAVMF TSYHNINYYS
     DFLYTSFNRS YALVVTQDKH VTVSANIDAG MPWRRSFDEN IVYTDWKRDN FLYAVKKVLN
     EGGFSSGRLG VENDHMTLDL RRQVQDALPN TELVDVSQAV MGHRMFKSDE EIDLIKNGAR
     IADIGGAAVV EAIREGVPEY EVALHGTEAM VREIARTYPH AELRDTWIWF QSGINTDGAH
     NWATSRKLQR GDILSLNCFP MIAGYYTALE RTLFLEEVSD RHLELWEINC KVHRRGLELI
     KPGARCMDIA AELNEIYREH DLLANRTFGY GHSFGVLSHY YGREAGLELR EDIETVLEPG
     MVVSMEPMIM IPEGEPGAGG YREHDILVIS ENGTENITKF PFGPEHNIIK K