ID   CREA_FLASU              Reviewed;         403 AA.
AC   P19213;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 2.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Creatinase;
DE            EC=3.5.3.3;
DE   AltName: Full=Creatine amidinohydrolase;
OS   Flavobacterium sp. (strain U-188).
OC   Bacteria; Bacteroidetes; Flavobacteriia; Flavobacteriales;
OC   Flavobacteriaceae; Flavobacterium; unclassified Flavobacterium.
OX   NCBI_TaxID=242;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-15.
RX   PubMed=1368564; DOI=10.1271/bbb1961.54.1453;
RA   Koyama Y., Kitao S., Yamamoto-Otake H., Suzuki M., Nakano E.;
RT   "Cloning and expression of the creatinase gene from Flavobacterium sp. U-
RT   188 in Escherichia coli.";
RL   Agric. Biol. Chem. 54:1453-1457(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Suzuki K., Sagai H., Sugiyama M., Imamura S.;
RT   "Molecular cloning and high expression of the Bacillus creatinase gene in
RT   Escherichia coli.";
RL   J. Ferment. Bioeng. 76:77-81(1993).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=creatine + H2O = sarcosine + urea; Xref=Rhea:RHEA:22456,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:16199, ChEBI:CHEBI:57433,
CC         ChEBI:CHEBI:57947; EC=3.5.3.3;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase M24 family. Creatinase subfamily.
CC       {ECO:0000305}.
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DR   EMBL; D00656; BAA32239.1; -; Genomic_DNA.
DR   EMBL; D14464; BAA03359.1; -; Genomic_DNA.
DR   PIR; JH0134; JH0134.
DR   AlphaFoldDB; P19213; -.
DR   SMR; P19213; -.
DR   GO; GO:0016980; F:creatinase activity; IEA:UniProtKB-EC.
DR   CDD; cd01090; Creatinase; 1.
DR   Gene3D; 3.40.350.10; -; 1.
DR   Gene3D; 3.90.230.10; -; 1.
DR   InterPro; IPR029149; Creatin/AminoP/Spt16_NTD.
DR   InterPro; IPR036005; Creatinase/aminopeptidase-like.
DR   InterPro; IPR039394; Creatinase_C.
DR   InterPro; IPR000587; Creatinase_N.
DR   InterPro; IPR000994; Pept_M24.
DR   Pfam; PF01321; Creatinase_N; 1.
DR   Pfam; PF00557; Peptidase_M24; 1.
DR   SUPFAM; SSF53092; SSF53092; 1.
DR   SUPFAM; SSF55920; SSF55920; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase.
FT   CHAIN           1..403
FT                   /note="Creatinase"
FT                   /id="PRO_0000079343"
FT   ACT_SITE        232
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   403 AA;  45780 MW;  754BDCCF456D962C CRC64;
     MQMPKTLRIR NGEKVKSTFS AQEYANRHAK LRAHLAAENI DAAVFTSYHN INYYSDFLYC
     SFGRPYALVV TQDDVISISA NIDGGQPWRR TVGTDNIVYT DWQRDNYFVA IQQALPRARR
     IGIEHDHLNL QNRDKLAARY PDAELVDVAA ACMRMRMIKS AEEHEMIRHG ARVADIGGAA
     IVEALRDQVP EYEVALHATQ AMVRAIAETF DNVELMDTWT WFQSGINTDG AHNPVTTRKV
     NKGDILSLNC FPMIAGYYTA LERTLFLDHC SDDHLRMWQA NVEVHEAGLK LIKPGMRCSD
     IAKELNEIFL KHDLLQYRTF GYGHSFGTLS HYYGREAGLE LREDIDTVLE PGMVVSMEPM
     IMLPEGRPGA GGYREHDILI VNENGAENIT KFPYGPERNI IRK