CREBA_DROME
ID CREBA_DROME Reviewed; 516 AA.
AC P29747; Q24282; Q64M72; Q8MQX0; Q9VUQ4;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2004, sequence version 2.
DT 03-AUG-2022, entry version 186.
DE RecName: Full=Cyclic AMP response element-binding protein A;
DE Short=cAMP response element-binding protein A;
DE Short=dCREB-A;
DE AltName: Full=Box B-binding factor 2;
DE Short=BBF-2;
GN Name=CrebA; Synonyms=Bbbf2; ORFNames=CG7450;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=Oregon-R; TISSUE=Embryo;
RX PubMed=1532159; DOI=10.1101/gad.6.3.466;
RA Abel T., Bhatt R., Maniatis T.;
RT "A Drosophila CREB/ATF transcriptional activator binds to both fat
RT body- and liver-specific regulatory elements.";
RL Genes Dev. 6:466-480(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Embryo;
RX PubMed=1508208; DOI=10.1128/mcb.12.9.4123-4131.1992;
RA Smolik S.M., Rose T.M., Goodman R.H.;
RT "A cyclic AMP-responsive element-binding transcriptional activator in
RT Drosophila melanogaster, dCREB-A, is a member of the leucine zipper
RT family.";
RL Mol. Cell. Biol. 12:4123-4131(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-18.
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [6]
RP SEQUENCE REVISION.
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 221-516.
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-75; SER-79 AND SER-82, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Transcriptional activator. Binds to fat body-specific
CC enhancers of alcohol dehydrogenase (ADH) and yolk protein genes. BBF-2
CC may play a role in fat body gene expression. It binds the consensus
CC sequence 5'-T[AC]NACGTAN[TG]C-3'. {ECO:0000269|PubMed:1508208,
CC ECO:0000269|PubMed:1532159}.
CC -!- SUBUNIT: May bind DNA as heterodimers with other bZIP proteins.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: In all cell types examined, including developing
CC salivary gland in embryos and in adults, brain and optic lobe cell
CC bodies, salivary gland, midgut epithelial cells of the cardia, female
CC ovarian columnar follicle cells and male seminal vesicle, ejaculatory
CC duct, and ejaculatory bulb. {ECO:0000269|PubMed:1508208}.
CC -!- DEVELOPMENTAL STAGE: Present throughout development.
CC -!- SIMILARITY: Belongs to the bZIP family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAU21396.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
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DR EMBL; X64429; CAA45771.1; -; mRNA.
DR EMBL; M87038; AAA28427.1; -; mRNA.
DR EMBL; AE014296; AAF49621.2; -; Genomic_DNA.
DR EMBL; AY122251; AAU21396.1; ALT_SEQ; mRNA.
DR EMBL; BT011074; AAR82739.1; -; mRNA.
DR PIR; A42140; A42140.
DR PIR; A44494; A44494.
DR RefSeq; NP_524087.3; NM_079363.5.
DR RefSeq; NP_996096.1; NM_206374.2.
DR AlphaFoldDB; P29747; -.
DR SMR; P29747; -.
DR BioGRID; 65001; 10.
DR ELM; P29747; -.
DR IntAct; P29747; 5.
DR STRING; 7227.FBpp0089339; -.
DR iPTMnet; P29747; -.
DR PaxDb; P29747; -.
DR PRIDE; P29747; -.
DR DNASU; 39682; -.
DR EnsemblMetazoa; FBtr0075557; FBpp0075311; FBgn0004396.
DR EnsemblMetazoa; FBtr0075558; FBpp0089339; FBgn0004396.
DR GeneID; 39682; -.
DR KEGG; dme:Dmel_CG7450; -.
DR CTD; 39682; -.
DR FlyBase; FBgn0004396; CrebA.
DR VEuPathDB; VectorBase:FBgn0004396; -.
DR eggNOG; KOG0709; Eukaryota.
DR HOGENOM; CLU_050111_1_0_1; -.
DR InParanoid; P29747; -.
DR OMA; SPDHDMH; -.
DR OrthoDB; 644485at2759; -.
DR PhylomeDB; P29747; -.
DR BioGRID-ORCS; 39682; 0 hits in 3 CRISPR screens.
DR ChiTaRS; CrebA; fly.
DR GenomeRNAi; 39682; -.
DR PRO; PR:P29747; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0004396; Expressed in spermathecum and 53 other tissues.
DR ExpressionAtlas; P29747; baseline and differential.
DR Genevisible; P29747; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0005700; C:polytene chromosome; IDA:FlyBase.
DR GO; GO:0035497; F:cAMP response element binding; IBA:GO_Central.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0003677; F:DNA binding; IDA:FlyBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0035293; P:chitin-based larval cuticle pattern formation; IMP:FlyBase.
DR GO; GO:0009953; P:dorsal/ventral pattern formation; IGI:FlyBase.
DR GO; GO:0008363; P:larval chitin-based cuticle development; IMP:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007431; P:salivary gland development; IMP:FlyBase.
DR InterPro; IPR004827; bZIP.
DR InterPro; IPR046347; bZIP_sf.
DR Pfam; PF00170; bZIP_1; 1.
DR SMART; SM00338; BRLZ; 1.
DR SUPFAM; SSF57959; SSF57959; 1.
DR PROSITE; PS50217; BZIP; 1.
DR PROSITE; PS00036; BZIP_BASIC; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Nucleus; Phosphoprotein; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..516
FT /note="Cyclic AMP response element-binding protein A"
FT /id="PRO_0000076635"
FT DOMAIN 441..504
FT /note="bZIP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 213..237
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 294..338
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 353..408
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 443..463
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 469..476
FT /note="Leucine-zipper"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT COMPBIAS 221..237
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 294..308
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 75
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 79
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 82
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT CONFLICT 114
FT /note="Y -> C (in Ref. 1; CAA45771 and 2; AAA28427)"
FT /evidence="ECO:0000305"
FT CONFLICT 277..278
FT /note="QQ -> HE (in Ref. 1; CAA45771)"
FT /evidence="ECO:0000305"
FT CONFLICT 305
FT /note="D -> G (in Ref. 1; CAA45771 and 7; AAR82739)"
FT /evidence="ECO:0000305"
FT CONFLICT 316
FT /note="L -> T (in Ref. 1; CAA45771)"
FT /evidence="ECO:0000305"
FT CONFLICT 338..364
FT /note="APLSPNATVSISVANPAGGESSVRVSR -> RHCRPTQPFPSLWPIQPAVSH
FT PYGSA (in Ref. 1; CAA45771)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 516 AA; 56342 MW; 25EA48DAE2ACE7E6 CRC64;
MEFYDGDLKD IWDSDLDPES LKISPDHDMH DWLFDRDVKD PTVILNDKLI SDALLNGTQP
IKTEHSYSLS SDVDSLPDSP KSLQAKIEDM DDECFPAISP KTATNGRVTI DPKYLTFHVP
PTHATPISRL SSNPALNTSV ADLTRSSGLQ SLQAHQPHHG SGSSHVVVAN LEHFQLPQHL
YDNDCSSSVS SLRDGSMSPD ICSDIEIDES AIKDEPMSPD SSCPASPTSQ ASSSQHQLSL
NLAHLQSEML FEPKHCGLLL TASSNSNNSL IKSQQRQQQI LGQDNLLMAK MEIKSEKQST
SNSSDKSHAH GYGIPLTPPS SLPSDDSEGN LSPEHLFAPL SPNATVSISV ANPAGGESSV
RVSRTAASIT RSSSGSASAS GSSTSSTVTT TRQPIHTPLI SSQPKGSTGT LLLTEEEKRT
LLAEGYPIPQ KLPLTKAEEK SLKKIRRKIK NKISAQESRR KKKEYMDQLE RRVEILVTEN
HDYKKRLEGL EETNANLLSQ LHKLQALVSK HNVKKS
