CREN7_SACS2
ID CREN7_SACS2 Reviewed; 60 AA.
AC Q97ZE3;
DT 22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 1.
DT 25-MAY-2022, entry version 86.
DE RecName: Full=Chromatin protein Cren7;
GN Name=creN7; OrderedLocusNames=SSO6901;
OS Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
OS (Sulfolobus solfataricus).
OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC Saccharolobus.
OX NCBI_TaxID=273057;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX PubMed=11427726; DOI=10.1073/pnas.141222098;
RA She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J.,
RA Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G.,
RA Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J.,
RA Medina N., Peng X., Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C.,
RA Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T.,
RA Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.;
RT "The complete genome of the crenarchaeon Sulfolobus solfataricus P2.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001).
RN [2]
RP FUNCTION IN DNA-BINDING, SUBUNIT, AND STRUCTURE BY NMR.
RC STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX PubMed=18096617; DOI=10.1093/nar/gkm1128;
RA Guo L., Feng Y., Zhang Z., Yao H., Luo Y., Wang J., Huang L.;
RT "Biochemical and structural characterization of Cren7, a novel chromatin
RT protein conserved among Crenarchaea.";
RL Nucleic Acids Res. 36:1129-1137(2008).
CC -!- FUNCTION: A highly abundant probable chromatin protein, it binds
CC double-strand DNA without sequence specificity; there is approximately
CC 1 Cren7 molecule for 12 bp of DNA. Constrains negative DNA supercoils,
CC increases DNA stability against thermal denaturation. Binding does not
CC require protein methylation. Binds single-strand DNA weakly.
CC {ECO:0000269|PubMed:18096617}.
CC -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:18096617}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- PTM: Methylated at multiple sites, to varying extents. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the Cren7 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE006641; AAK41249.1; -; Genomic_DNA.
DR PIR; B90249; B90249.
DR RefSeq; WP_009992425.1; NC_002754.1.
DR PDB; 2JTM; NMR; -; A=1-60.
DR PDB; 3KXT; X-ray; 1.60 A; A=6-60.
DR PDB; 3LWH; X-ray; 1.90 A; A=1-60.
DR PDB; 3LWI; X-ray; 2.30 A; A/B=1-60.
DR PDB; 4R55; X-ray; 1.80 A; A=1-60.
DR PDB; 4R56; X-ray; 2.30 A; A/B=1-60.
DR PDB; 5K07; X-ray; 2.00 A; A=1-60.
DR PDB; 5K17; X-ray; 2.10 A; A/B=1-60.
DR PDB; 5WVW; X-ray; 1.80 A; A/B=1-60.
DR PDB; 5WVY; X-ray; 2.00 A; A/B=1-60.
DR PDB; 5WVZ; X-ray; 2.30 A; A/B=1-60.
DR PDB; 5WWC; X-ray; 1.90 A; A/B=1-60.
DR PDB; 6A2H; X-ray; 2.30 A; A=1-60.
DR PDB; 6A2I; X-ray; 2.40 A; A/B=1-60.
DR PDBsum; 2JTM; -.
DR PDBsum; 3KXT; -.
DR PDBsum; 3LWH; -.
DR PDBsum; 3LWI; -.
DR PDBsum; 4R55; -.
DR PDBsum; 4R56; -.
DR PDBsum; 5K07; -.
DR PDBsum; 5K17; -.
DR PDBsum; 5WVW; -.
DR PDBsum; 5WVY; -.
DR PDBsum; 5WVZ; -.
DR PDBsum; 5WWC; -.
DR PDBsum; 6A2H; -.
DR PDBsum; 6A2I; -.
DR AlphaFoldDB; Q97ZE3; -.
DR SMR; Q97ZE3; -.
DR STRING; 273057.SSO6901; -.
DR EnsemblBacteria; AAK41249; AAK41249; SSO6901.
DR GeneID; 44129907; -.
DR KEGG; sso:SSO6901; -.
DR PATRIC; fig|273057.12.peg.974; -.
DR eggNOG; arCOG04114; Archaea.
DR HOGENOM; CLU_2911298_0_0_2; -.
DR OMA; LAPEKAW; -.
DR EvolutionaryTrace; Q97ZE3; -.
DR Proteomes; UP000001974; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR Gene3D; 2.30.30.610; -; 1.
DR HAMAP; MF_01387; Chromatin_Cren7; 1.
DR InterPro; IPR038647; Cren7_sf.
DR InterPro; IPR020906; dsDNA-bd_Cren7.
DR Pfam; PF11520; Cren7; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; DNA-binding; Methylation; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..60
FT /note="Chromatin protein Cren7"
FT /id="PRO_0000345178"
FT STRAND 8..11
FT /evidence="ECO:0007829|PDB:3KXT"
FT STRAND 17..20
FT /evidence="ECO:0007829|PDB:3KXT"
FT STRAND 23..28
FT /evidence="ECO:0007829|PDB:3KXT"
FT STRAND 36..42
FT /evidence="ECO:0007829|PDB:3KXT"
FT TURN 44..46
FT /evidence="ECO:0007829|PDB:3KXT"
FT STRAND 49..53
FT /evidence="ECO:0007829|PDB:3KXT"
SQ SEQUENCE 60 AA; 6663 MW; 665BC5BE3FA53C8D CRC64;
MSSGKKPVKV KTPAGKEAEL VPEKVWALAP KGRKGVKIGL FKDPETGKYF RHKLPDDYPI