CRF1_YEAST
ID CRF1_YEAST Reviewed; 467 AA.
AC Q04930; D6VSK5;
DT 21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Transcription factor CRF1;
DE AltName: Full=Corepressor with FHL1 protein 1;
GN Name=CRF1; OrderedLocusNames=YDR223W; ORFNames=YD9934.08;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204511 / S288c / AB972;
RX PubMed=8885406; DOI=10.1099/13500872-142-10-2897;
RA de Virgilio C., DeMarini D.J., Pringle J.R.;
RT "SPR28, a sixth member of the septin gene family in Saccharomyces
RT cerevisiae that is expressed specifically in sporulating cells.";
RL Microbiology 142:2897-2905(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP PHOSPHORYLATION BY CDC28.
RX PubMed=14574415; DOI=10.1038/nature02062;
RA Ubersax J.A., Woodbury E.L., Quang P.N., Paraz M., Blethrow J.D., Shah K.,
RA Shokat K.M., Morgan D.O.;
RT "Targets of the cyclin-dependent kinase Cdk1.";
RL Nature 425:859-864(2003).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH FHL1, AND PHOSPHORYLATION
RP BY YAK1.
RX PubMed=15620355; DOI=10.1016/j.cell.2004.11.047;
RA Martin D.E., Soulard A., Hall M.N.;
RT "TOR regulates ribosomal protein gene expression via PKA and the forkhead
RT transcription factor FHL1.";
RL Cell 119:969-979(2004).
RN [6]
RP FUNCTION.
RX PubMed=14966276; DOI=10.1128/mcb.24.5.1956-1967.2004;
RA Oki M., Valenzuela L., Chiba T., Ito T., Kamakaka R.T.;
RT "Barrier proteins remodel and modify chromatin to restrict silenced
RT domains.";
RL Mol. Cell. Biol. 24:1956-1967(2004).
CC -!- FUNCTION: Transcription factor, corepressor with FHL1 of ribosomal
CC protein genes. May be involved in the blocking of the spread of
CC silencing. {ECO:0000269|PubMed:14966276, ECO:0000269|PubMed:15620355}.
CC -!- SUBUNIT: Interacts with FHL1 to form a repressor complex. The formation
CC of the CRF1-FHL1 complex is inhibited by the TOR pathway.
CC {ECO:0000269|PubMed:15620355}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15620355}. Nucleus
CC {ECO:0000269|PubMed:15620355}. Note=The nuclear location is negatively
CC regulated by the TOR pathway.
CC -!- PTM: Phosphorylated by CDC28 and YAK1. {ECO:0000269|PubMed:14574415,
CC ECO:0000269|PubMed:15620355}.
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DR EMBL; Z48612; CAA88503.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA12065.1; -; Genomic_DNA.
DR PIR; S59430; S59430.
DR RefSeq; NP_010509.1; NM_001180531.1.
DR AlphaFoldDB; Q04930; -.
DR BioGRID; 32275; 27.
DR DIP; DIP-8620N; -.
DR IntAct; Q04930; 1.
DR STRING; 4932.YDR223W; -.
DR PaxDb; Q04930; -.
DR PRIDE; Q04930; -.
DR EnsemblFungi; YDR223W_mRNA; YDR223W; YDR223W.
DR GeneID; 851809; -.
DR KEGG; sce:YDR223W; -.
DR SGD; S000002631; CRF1.
DR VEuPathDB; FungiDB:YDR223W; -.
DR GeneTree; ENSGT00940000176808; -.
DR HOGENOM; CLU_585461_0_0_1; -.
DR InParanoid; Q04930; -.
DR OMA; LEKXYSS; -.
DR BioCyc; YEAST:G3O-29803-MON; -.
DR PRO; PR:Q04930; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q04930; protein.
DR GO; GO:0000785; C:chromatin; IDA:SGD.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0003714; F:transcription corepressor activity; IDA:SGD.
DR GO; GO:0010688; P:negative regulation of ribosomal protein gene transcription by RNA polymerase II; IMP:SGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR018837; TF_CRF1/IFH1.
DR PANTHER; PTHR28057; PTHR28057; 3.
DR Pfam; PF10380; CRF1; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Phosphoprotein; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..467
FT /note="Transcription factor CRF1"
FT /id="PRO_0000227691"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 62..110
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 151..170
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 341..361
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 24..42
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 75..99
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 151..169
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 467 AA; 51697 MW; 026A685D9DF78884 CRC64;
MLLSAPVNST VRRKPHSPNK KKPKETGTAA SFSSSSSTVV LSSNNDGSFD ALWDPSISKA
SDFESSYISA KRLKPESSNR QKKKNSYKYS REENTNEVEE KTSLGSSSKT EADNIFNDQL
TSAGNTTYVS NKRDVNFGAN SAVVLLGLPT SKSESHRQYH SPSASTTNED EEDIGVDILV
DNHIDSCETV SINNNRGITH QYPETESDVD FDEAVILTPM DGTDKGVKNP RPLEKKYSSS
CFEDRTPLNL DDGHFSECNH FSTLDVSSFF HLNEHVHKID EVELDGPDRT FSLDNVAINT
RKKDIDCLYN SSREDLSNLT CSSEGPRNDS YDSDYNIDEV TYRDDESTDE DESLPTPDRK
RKKIGHKACE ILDSKRIGIK VPKLYVWSLS DKPFSVIDGL CTKSLYPLSD DINTPESLSS
CSSSVSSREN QKGDATFDND AMIADLLNIG GLEVEKASNG HIELIGE
