CRF2_ARATH
ID CRF2_ARATH Reviewed; 343 AA.
AC Q9SUQ2; Q8L701;
DT 12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Ethylene-responsive transcription factor CRF2;
DE AltName: Full=Protein CYTOKININ RESPONSE FACTOR 2;
GN Name=CRF2; Synonyms=ERF064; OrderedLocusNames=At4g23750;
GN ORFNames=F9D16.220;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, INDUCTION BY CYTOKININS, AND SUBCELLULAR LOCATION.
RX PubMed=16832061; DOI=10.1073/pnas.0602038103;
RA Rashotte A.M., Mason M.G., Hutchison C.E., Ferreira F.J., Schaller G.E.,
RA Kieber J.J.;
RT "A subset of Arabidopsis AP2 transcription factors mediates cytokinin
RT responses in concert with a two-component pathway.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11081-11085(2006).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16407444; DOI=10.1104/pp.105.073783;
RA Nakano T., Suzuki K., Fujimura T., Shinshi H.;
RT "Genome-wide analysis of the ERF gene family in Arabidopsis and rice.";
RL Plant Physiol. 140:411-432(2006).
CC -!- FUNCTION: Component of the cytokinin signaling pathway involved in
CC cotyledons, leaves, and embryos development. Probably acts as a
CC transcriptional activator. Binds to the GCC-box pathogenesis-related
CC promoter element. May be involved in the regulation of gene expression
CC by stress factors and by components of stress signal transduction
CC pathways (By similarity). {ECO:0000250, ECO:0000269|PubMed:16832061}.
CC -!- INTERACTION:
CC Q9SUQ2; O82503: CRF1; NbExp=3; IntAct=EBI-5567273, EBI-5567050;
CC Q9SUQ2; Q9SUQ2: CRF2; NbExp=3; IntAct=EBI-5567273, EBI-5567273;
CC Q9SUQ2; Q9FK12: CRF3; NbExp=5; IntAct=EBI-5567273, EBI-5567993;
CC Q9SUQ2; Q9SUE3: CRF4; NbExp=4; IntAct=EBI-5567273, EBI-5567027;
CC Q9SUQ2; O82339: CRF5; NbExp=4; IntAct=EBI-5567273, EBI-5567180;
CC Q9SUQ2; Q9M374: CRF6; NbExp=4; IntAct=EBI-5567273, EBI-5568101;
CC Q9SUQ2; Q8W4I5: ERF069; NbExp=3; IntAct=EBI-5567273, EBI-5568301;
CC Q9SUQ2; Q9C995: ERF070; NbExp=4; IntAct=EBI-5567273, EBI-5568333;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16832061}. Nucleus
CC {ECO:0000255|PROSITE-ProRule:PRU00366, ECO:0000269|PubMed:16832061}.
CC Note=Relocalization from the cytoplasm into the nucleus is induced by
CC cytokinins.
CC -!- INDUCTION: By cytokinins. {ECO:0000269|PubMed:16832061}.
CC -!- SIMILARITY: Belongs to the AP2/ERF transcription factor family. ERF
CC subfamily. {ECO:0000305}.
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DR EMBL; AL035394; CAA23041.1; -; Genomic_DNA.
DR EMBL; AL161560; CAB81293.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE84801.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE84802.1; -; Genomic_DNA.
DR EMBL; AY057475; AAL09709.1; -; mRNA.
DR EMBL; AY140049; AAM98190.1; -; mRNA.
DR EMBL; BT015038; AAT70489.1; -; mRNA.
DR PIR; T05607; T05607.
DR RefSeq; NP_194106.1; NM_118506.3.
DR RefSeq; NP_974599.1; NM_202870.1.
DR AlphaFoldDB; Q9SUQ2; -.
DR SMR; Q9SUQ2; -.
DR BioGRID; 13764; 14.
DR IntAct; Q9SUQ2; 15.
DR STRING; 3702.AT4G23750.1; -.
DR iPTMnet; Q9SUQ2; -.
DR PaxDb; Q9SUQ2; -.
DR PRIDE; Q9SUQ2; -.
DR ProteomicsDB; 224496; -.
DR EnsemblPlants; AT4G23750.1; AT4G23750.1; AT4G23750.
DR EnsemblPlants; AT4G23750.2; AT4G23750.2; AT4G23750.
DR GeneID; 828475; -.
DR Gramene; AT4G23750.1; AT4G23750.1; AT4G23750.
DR Gramene; AT4G23750.2; AT4G23750.2; AT4G23750.
DR KEGG; ath:AT4G23750; -.
DR Araport; AT4G23750; -.
DR TAIR; locus:2128429; AT4G23750.
DR eggNOG; ENOG502R7AV; Eukaryota.
DR HOGENOM; CLU_062946_1_0_1; -.
DR InParanoid; Q9SUQ2; -.
DR OMA; RIKFTEH; -.
DR OrthoDB; 1269518at2759; -.
DR PhylomeDB; Q9SUQ2; -.
DR PRO; PR:Q9SUQ2; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SUQ2; baseline and differential.
DR Genevisible; Q9SUQ2; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0048825; P:cotyledon development; IMP:TAIR.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0048364; P:root development; IEP:TAIR.
DR CDD; cd00018; AP2; 1.
DR Gene3D; 3.30.730.10; -; 1.
DR InterPro; IPR001471; AP2/ERF_dom.
DR InterPro; IPR036955; AP2/ERF_dom_sf.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR Pfam; PF00847; AP2; 1.
DR PRINTS; PR00367; ETHRSPELEMNT.
DR SMART; SM00380; AP2; 1.
DR SUPFAM; SSF54171; SSF54171; 1.
DR PROSITE; PS51032; AP2_ERF; 1.
PE 1: Evidence at protein level;
KW Activator; Cytokinin signaling pathway; Cytoplasm; DNA-binding;
KW Ethylene signaling pathway; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..343
FT /note="Ethylene-responsive transcription factor CRF2"
FT /id="PRO_0000290401"
FT DNA_BIND 121..178
FT /note="AP2/ERF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT REGION 40..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 180..223
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 207..223
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 95
FT /note="R -> K (in Ref. 3; AAM98190)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 343 AA; 37873 MW; 85486A1172CAE9A9 CRC64;
MEAEKKMVLP RIKFTEHKTN TTTIVSELTN THQTRILRIS VTDPDATDSS SDDEEEEHQR
FVSKRRRVKK FVNEVYLDSG AVVTGSCGQM ESKKRQKRAV KSESTVSPVV SATTTTTGEK
KFRGVRQRPW GKWAAEIRDP LKRVRLWLGT YNTAEEAAMV YDNAAIQLRG PDALTNFSVT
PTTATEKKAP PPSPVKKKKK KNNKSKKSVT ASSSISRSSS NDCLCSPVSV LRSPFAVDEF
SGISSSPVAA VVVKEEPSMT TVSETFSDFS APLFSDDDVF DFRSSVVPDY LGGDLFGEDL
FTADMCTDMN FGFDFGSGLS SWHMEDHFQD IGDLFGSDPL LAV
