CRF3_ARATH
ID CRF3_ARATH Reviewed; 354 AA.
AC Q9FK12;
DT 12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Ethylene-responsive transcription factor CRF3;
DE AltName: Full=Protein CYTOKININ RESPONSE FACTOR 3;
GN Name=CRF3; Synonyms=ERF065; OrderedLocusNames=At5g53290; ORFNames=K19E1.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Pan Y., Gong W., Liu D., Fu Q., Mei W.-Q., Song W.-Q., Ma L.-G., Luo J.-C.,
RA Deng X.-W., Zhu Y.-X.;
RT "Molecular cloning, expression, phylogenetic and functional
RT characterization of the Arabidopsis AP2/EREBP transcription factor
RT family.";
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9734815; DOI=10.1093/dnares/5.3.203;
RA Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence
RT features of the regions of 1,367,185 bp covered by 19 physically assigned
RT P1 and TAC clones.";
RL DNA Res. 5:203-216(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=16832061; DOI=10.1073/pnas.0602038103;
RA Rashotte A.M., Mason M.G., Hutchison C.E., Ferreira F.J., Schaller G.E.,
RA Kieber J.J.;
RT "A subset of Arabidopsis AP2 transcription factors mediates cytokinin
RT responses in concert with a two-component pathway.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11081-11085(2006).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16407444; DOI=10.1104/pp.105.073783;
RA Nakano T., Suzuki K., Fujimura T., Shinshi H.;
RT "Genome-wide analysis of the ERF gene family in Arabidopsis and rice.";
RL Plant Physiol. 140:411-432(2006).
CC -!- FUNCTION: Component of the cytokinin signaling pathway involved in
CC cotyledons, leaves, and embryos development. Probably acts as a
CC transcriptional activator. Binds to the GCC-box pathogenesis-related
CC promoter element. May be involved in the regulation of gene expression
CC by stress factors and by components of stress signal transduction
CC pathways (By similarity). {ECO:0000250, ECO:0000269|PubMed:16832061}.
CC -!- INTERACTION:
CC Q9FK12; O82503: CRF1; NbExp=3; IntAct=EBI-5567993, EBI-5567050;
CC Q9FK12; Q9SUQ2: CRF2; NbExp=5; IntAct=EBI-5567993, EBI-5567273;
CC Q9FK12; Q9SUE3: CRF4; NbExp=4; IntAct=EBI-5567993, EBI-5567027;
CC Q9FK12; O82339: CRF5; NbExp=4; IntAct=EBI-5567993, EBI-5567180;
CC Q9FK12; Q9M374: CRF6; NbExp=3; IntAct=EBI-5567993, EBI-5568101;
CC Q9FK12; Q8W4I5: ERF069; NbExp=3; IntAct=EBI-5567993, EBI-5568301;
CC Q9FK12; Q9C995: ERF070; NbExp=4; IntAct=EBI-5567993, EBI-5568333;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16832061}. Nucleus
CC {ECO:0000255|PROSITE-ProRule:PRU00366, ECO:0000269|PubMed:16832061}.
CC Note=Relocalization from the cytoplasm into the nucleus is induced by
CC cytokinins.
CC -!- SIMILARITY: Belongs to the AP2/ERF transcription factor family. ERF
CC subfamily. {ECO:0000305}.
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DR EMBL; AY560878; AAT44945.1; -; mRNA.
DR EMBL; AB013388; BAB09791.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96333.1; -; Genomic_DNA.
DR EMBL; BT020244; AAV74238.1; -; mRNA.
DR EMBL; BT021136; AAX22271.1; -; mRNA.
DR RefSeq; NP_200141.1; NM_124708.3.
DR AlphaFoldDB; Q9FK12; -.
DR SMR; Q9FK12; -.
DR BioGRID; 20655; 48.
DR IntAct; Q9FK12; 48.
DR STRING; 3702.AT5G53290.1; -.
DR PaxDb; Q9FK12; -.
DR PRIDE; Q9FK12; -.
DR ProteomicsDB; 220447; -.
DR EnsemblPlants; AT5G53290.1; AT5G53290.1; AT5G53290.
DR GeneID; 835410; -.
DR Gramene; AT5G53290.1; AT5G53290.1; AT5G53290.
DR KEGG; ath:AT5G53290; -.
DR Araport; AT5G53290; -.
DR TAIR; locus:2154272; AT5G53290.
DR eggNOG; ENOG502R7AV; Eukaryota.
DR HOGENOM; CLU_062946_1_0_1; -.
DR InParanoid; Q9FK12; -.
DR OMA; MSIFDQP; -.
DR OrthoDB; 1326338at2759; -.
DR PhylomeDB; Q9FK12; -.
DR PRO; PR:Q9FK12; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FK12; baseline and differential.
DR Genevisible; Q9FK12; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0048825; P:cotyledon development; IMP:TAIR.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0048366; P:leaf development; IMP:TAIR.
DR CDD; cd00018; AP2; 1.
DR Gene3D; 3.30.730.10; -; 1.
DR InterPro; IPR001471; AP2/ERF_dom.
DR InterPro; IPR036955; AP2/ERF_dom_sf.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR Pfam; PF00847; AP2; 1.
DR PRINTS; PR00367; ETHRSPELEMNT.
DR SMART; SM00380; AP2; 1.
DR SUPFAM; SSF54171; SSF54171; 1.
DR PROSITE; PS51032; AP2_ERF; 1.
PE 1: Evidence at protein level;
KW Activator; Cytokinin signaling pathway; Cytoplasm; DNA-binding;
KW Ethylene signaling pathway; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..354
FT /note="Ethylene-responsive transcription factor CRF3"
FT /id="PRO_0000290402"
FT DNA_BIND 124..181
FT /note="AP2/ERF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT REGION 85..120
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 180..220
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 99..120
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 204..220
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 354 AA; 40100 MW; 98975667D87293EA CRC64;
MDEYIDFRPL KYTEHKTSMT KYTKKSSEKL SGGKSLKKVS ICYTDPDATD SSSDEDEEDF
LFPRRRVKRF VNEITVEPSC NNVVTGVSMK DRKRLSSSSD ETQSPASSRQ RPNNKVSVSG
QIKKFRGVRQ RPWGKWAAEI RDPEQRRRIW LGTFETAEEA AVVYDNAAIR LRGPDALTNF
SIPPQEEEEE EEPEPVIEEK PVIMTTPTPT TSSSESTEED LQHLSSPTSV LNHRSEEIQQ
VQQPFKSAKP EPGVSNAPWW HTGFNTGLGE SDDSFPLDTP FLDNYFNESP PEMSIFDQPM
DQIFCENDDI FNDMLFLGGE TMNIEDELTS SSIKDMGSTF SDFDDSLISD LLVA
