CRF4_ARATH
ID CRF4_ARATH Reviewed; 335 AA.
AC Q9SUE3; B3LFA0;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 31-MAY-2011, sequence version 2.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Ethylene-responsive transcription factor CRF4;
DE AltName: Full=Protein CYTOKININ RESPONSE FACTOR 4;
GN Name=CRF4; Synonyms=ERF066; OrderedLocusNames=At4g27950; ORFNames=T13J8.60;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA De Los Reyes C., Quan R., Chen H., Bautista V.R., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 2-335.
RA Pan Y., Gong W., Liu D., Fu Q., Mei W.-Q., Song W.-Q., Ma L.-G., Luo J.-C.,
RA Deng X.-W., Zhu Y.-X.;
RT "Molecular cloning, expression, phylogenetic and functional
RT characterization of the Arabidopsis AP2/EREBP transcription factor
RT family.";
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=16832061; DOI=10.1073/pnas.0602038103;
RA Rashotte A.M., Mason M.G., Hutchison C.E., Ferreira F.J., Schaller G.E.,
RA Kieber J.J.;
RT "A subset of Arabidopsis AP2 transcription factors mediates cytokinin
RT responses in concert with a two-component pathway.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11081-11085(2006).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16407444; DOI=10.1104/pp.105.073783;
RA Nakano T., Suzuki K., Fujimura T., Shinshi H.;
RT "Genome-wide analysis of the ERF gene family in Arabidopsis and rice.";
RL Plant Physiol. 140:411-432(2006).
CC -!- FUNCTION: Component of the cytokinin signaling pathway involved in
CC cotyledons, leaves, and embryos development. Probably acts as a
CC transcriptional activator. Binds to the GCC-box pathogenesis-related
CC promoter element. May be involved in the regulation of gene expression
CC by stress factors and by components of stress signal transduction
CC pathways (By similarity). {ECO:0000250, ECO:0000269|PubMed:16832061}.
CC -!- INTERACTION:
CC Q9SUE3; O82503: CRF1; NbExp=4; IntAct=EBI-5567027, EBI-5567050;
CC Q9SUE3; Q9SUQ2: CRF2; NbExp=4; IntAct=EBI-5567027, EBI-5567273;
CC Q9SUE3; Q9FK12: CRF3; NbExp=4; IntAct=EBI-5567027, EBI-5567993;
CC Q9SUE3; O82339: CRF5; NbExp=4; IntAct=EBI-5567027, EBI-5567180;
CC Q9SUE3; Q9M374: CRF6; NbExp=4; IntAct=EBI-5567027, EBI-5568101;
CC Q9SUE3; Q8W4I5: ERF069; NbExp=3; IntAct=EBI-5567027, EBI-5568301;
CC Q9SUE3; Q9C995: ERF070; NbExp=3; IntAct=EBI-5567027, EBI-5568333;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16832061}. Nucleus
CC {ECO:0000255|PROSITE-ProRule:PRU00366, ECO:0000269|PubMed:16832061}.
CC Note=Relocalization from the cytoplasm into the nucleus is induced by
CC cytokinins.
CC -!- SIMILARITY: Belongs to the AP2/ERF transcription factor family. ERF
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB36764.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAB79597.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL035524; CAB36764.1; ALT_INIT; Genomic_DNA.
DR EMBL; AL161572; CAB79597.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002687; AEE85412.1; -; Genomic_DNA.
DR EMBL; BT033088; ACF04811.1; -; mRNA.
DR EMBL; AY560872; AAT44939.1; -; mRNA.
DR PIR; T02896; T02896.
DR RefSeq; NP_194524.2; NM_118933.3.
DR AlphaFoldDB; Q9SUE3; -.
DR SMR; Q9SUE3; -.
DR BioGRID; 14195; 17.
DR IntAct; Q9SUE3; 17.
DR STRING; 3702.AT4G27950.1; -.
DR PaxDb; Q9SUE3; -.
DR PRIDE; Q9SUE3; -.
DR EnsemblPlants; AT4G27950.1; AT4G27950.1; AT4G27950.
DR GeneID; 828908; -.
DR Gramene; AT4G27950.1; AT4G27950.1; AT4G27950.
DR KEGG; ath:AT4G27950; -.
DR Araport; AT4G27950; -.
DR TAIR; locus:2132962; AT4G27950.
DR eggNOG; ENOG502R7AV; Eukaryota.
DR HOGENOM; CLU_062946_1_0_1; -.
DR InParanoid; Q9SUE3; -.
DR OMA; HECQGET; -.
DR OrthoDB; 1326338at2759; -.
DR PRO; PR:Q9SUE3; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SUE3; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0048366; P:leaf development; IMP:TAIR.
DR CDD; cd00018; AP2; 1.
DR Gene3D; 3.30.730.10; -; 1.
DR InterPro; IPR001471; AP2/ERF_dom.
DR InterPro; IPR036955; AP2/ERF_dom_sf.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR Pfam; PF00847; AP2; 1.
DR PRINTS; PR00367; ETHRSPELEMNT.
DR SMART; SM00380; AP2; 1.
DR SUPFAM; SSF54171; SSF54171; 1.
DR PROSITE; PS51032; AP2_ERF; 1.
PE 1: Evidence at protein level;
KW Activator; Cytokinin signaling pathway; Cytoplasm; DNA-binding;
KW Ethylene signaling pathway; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..335
FT /note="Ethylene-responsive transcription factor CRF4"
FT /id="PRO_0000297919"
FT DNA_BIND 117..174
FT /note="AP2/ERF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT REGION 78..121
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 175..209
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 103..118
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 176..209
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 335 AA; 38448 MW; CA487909D2D1E318 CRC64;
MMMDEFMDLR PVKYTEHKTV IRKYTKKSSM ERKTSVRDSA RLVRVSMTDR DATDSSSDEE
EFLFPRRRVK RLINEIRVEP SSSSTGDVSA SPTKDRKRIN VDSTVQKPSV SGQNQKKYRG
VRQRPWGKWA AEIRDPEQRR RIWLGTFATA EEAAIVYDNA AIKLRGPDAL TNFTVQPEPE
PVQEQEQEPE SNMSVSISES MDDSQHLSSP TSVLNYQTYV SEEPIDSLIK PVKQEFLEPE
QEPISWHLGE GNTNTNDDSF PLDITFLDNY FNESLPDISI FDQPMSPIQP TENDFFNDLM
LFDSNAEEYY SSEIKEIGSS FNDLDDSLIS DLLLV
