CRF5_ARATH
ID CRF5_ARATH Reviewed; 294 AA.
AC O82339;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Ethylene-responsive transcription factor CRF5;
DE AltName: Full=Protein CYTOKININ RESPONSE FACTOR 5;
GN Name=CRF5; Synonyms=ERF068; OrderedLocusNames=At2g46310; ORFNames=T3F17.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, INDUCTION BY CYTOKININS, AND SUBCELLULAR LOCATION.
RX PubMed=16832061; DOI=10.1073/pnas.0602038103;
RA Rashotte A.M., Mason M.G., Hutchison C.E., Ferreira F.J., Schaller G.E.,
RA Kieber J.J.;
RT "A subset of Arabidopsis AP2 transcription factors mediates cytokinin
RT responses in concert with a two-component pathway.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11081-11085(2006).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16407444; DOI=10.1104/pp.105.073783;
RA Nakano T., Suzuki K., Fujimura T., Shinshi H.;
RT "Genome-wide analysis of the ERF gene family in Arabidopsis and rice.";
RL Plant Physiol. 140:411-432(2006).
CC -!- FUNCTION: Component of the cytokinin signaling pathway involved in
CC cotyledons, leaves, and embryos development. Probably acts as a
CC transcriptional activator. Binds to the GCC-box pathogenesis-related
CC promoter element. May be involved in the regulation of gene expression
CC by stress factors and by components of stress signal transduction
CC pathways (By similarity). {ECO:0000250, ECO:0000269|PubMed:16832061}.
CC -!- INTERACTION:
CC O82339; O82503: CRF1; NbExp=3; IntAct=EBI-5567180, EBI-5567050;
CC O82339; Q9SUQ2: CRF2; NbExp=4; IntAct=EBI-5567180, EBI-5567273;
CC O82339; Q9FK12: CRF3; NbExp=4; IntAct=EBI-5567180, EBI-5567993;
CC O82339; Q9SUE3: CRF4; NbExp=4; IntAct=EBI-5567180, EBI-5567027;
CC O82339; Q9M374: CRF6; NbExp=3; IntAct=EBI-5567180, EBI-5568101;
CC O82339; Q8W4I5: ERF069; NbExp=3; IntAct=EBI-5567180, EBI-5568301;
CC O82339; Q9C995: ERF070; NbExp=3; IntAct=EBI-5567180, EBI-5568333;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16832061}. Nucleus
CC {ECO:0000255|PROSITE-ProRule:PRU00366, ECO:0000269|PubMed:16832061}.
CC Note=Relocalization from the cytoplasm into the nucleus is induced by
CC cytokinins.
CC -!- INDUCTION: By cytokinins. {ECO:0000269|PubMed:16832061}.
CC -!- SIMILARITY: Belongs to the AP2/ERF transcription factor family. ERF
CC subfamily. {ECO:0000305}.
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DR EMBL; AC005397; AAC62875.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10675.1; -; Genomic_DNA.
DR EMBL; AY074638; AAL69454.1; -; mRNA.
DR EMBL; BT003121; AAO24553.1; -; mRNA.
DR EMBL; AK227661; BAE99648.1; -; mRNA.
DR PIR; C84901; C84901.
DR RefSeq; NP_182154.1; NM_130194.5.
DR AlphaFoldDB; O82339; -.
DR SMR; O82339; -.
DR BioGRID; 4574; 14.
DR IntAct; O82339; 14.
DR STRING; 3702.AT2G46310.1; -.
DR PaxDb; O82339; -.
DR PRIDE; O82339; -.
DR ProteomicsDB; 222675; -.
DR EnsemblPlants; AT2G46310.1; AT2G46310.1; AT2G46310.
DR GeneID; 819239; -.
DR Gramene; AT2G46310.1; AT2G46310.1; AT2G46310.
DR KEGG; ath:AT2G46310; -.
DR Araport; AT2G46310; -.
DR TAIR; locus:2062954; AT2G46310.
DR eggNOG; ENOG502RYD1; Eukaryota.
DR HOGENOM; CLU_062946_1_0_1; -.
DR InParanoid; O82339; -.
DR OMA; LPEVECK; -.
DR OrthoDB; 1430404at2759; -.
DR PhylomeDB; O82339; -.
DR PRO; PR:O82339; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O82339; baseline and differential.
DR Genevisible; O82339; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0048825; P:cotyledon development; IMP:TAIR.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0048366; P:leaf development; IMP:TAIR.
DR CDD; cd00018; AP2; 1.
DR Gene3D; 3.30.730.10; -; 1.
DR InterPro; IPR001471; AP2/ERF_dom.
DR InterPro; IPR036955; AP2/ERF_dom_sf.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR Pfam; PF00847; AP2; 1.
DR PRINTS; PR00367; ETHRSPELEMNT.
DR SMART; SM00380; AP2; 1.
DR SUPFAM; SSF54171; SSF54171; 1.
DR PROSITE; PS51032; AP2_ERF; 1.
PE 1: Evidence at protein level;
KW Activator; Cytokinin signaling pathway; Cytoplasm; DNA-binding;
KW Ethylene signaling pathway; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..294
FT /note="Ethylene-responsive transcription factor CRF5"
FT /id="PRO_0000297920"
FT DNA_BIND 98..155
FT /note="AP2/ERF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT REGION 35..54
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 64..87
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 64..82
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 294 AA; 33156 MW; 5CBE0C513838C9E1 CRC64;
MKSRVRKSKY TVHRKITSTP FDGFPKIVKI IVTDPCATDS SSDEENDNKS VAPRVKRYVD
EIRFCDEDDE PKPARKAKKK SPAAAAENGG DLVKSVVKYR GVRQRPWGKF AAEIRDPSSR
TRLWLGTFAT AEEAAIGYDR AAIRIKGHNA QTNFLTPPPS PTTEVLPETP VIDLETVSGC
DSARESQISL CSPTSVLRFS HNDETEYRTE PTEEQNPFFL PDLFRSGDYF WDSEITPDPL
FLDEFHQSLL PNINNNNTVC DKDTNLSDSF PLGVIGDFSS WDVDEFFQDH LLDK
