ID   CRFA1_CONEP             Reviewed;          59 AA.
AC   P0DUQ4;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   29-SEP-2021, sequence version 1.
DT   23-FEB-2022, entry version 2.
DE   RecName: Full=Conorfamide-Ep1 {ECO:0000250|UniProtKB:P0DOZ7, ECO:0000303|PubMed:33764053};
DE            Short=CNF-Ep1 {ECO:0000303|PubMed:33764053};
DE   AltName: Full=Cono-RFamide-Ep1 {ECO:0000250|UniProtKB:P0DOZ7, ECO:0000303|PubMed:33764053};
DE   Flags: Precursor;
OS   Conus episcopatus (Bishop's cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Darioconus.
OX   NCBI_TaxID=88764;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], SYNTHESIS OF 26-43, FUNCTION, AND AMIDATION AT
RP   ILE-43.
RC   TISSUE=Venom duct;
RX   PubMed=33764053; DOI=10.1021/acs.jnatprod.0c01297;
RA   Bosse G.D., Urcino C., Watkins M., Florez Salcedo P., Kozel S., Chase K.,
RA   Cabang A., Espino S.S., Safavi-Hemami H., Raghuraman S., Olivera B.M.,
RA   Peterson R.T., Gajewiak J.;
RT   "Discovery of a potent conorfamide from Conus episcopatus using a novel
RT   zebrafish larvae assay.";
RL   J. Nat. Prod. 84:1232-1243(2021).
CC   -!- FUNCTION: Neurotoxin that is active on vertebrates (PubMed:33764053).
CC       When tested at high doses (10 uM), the toxin affects all zebrafish and
CC       mouse DRG neurons in culture, which could be an indication of an effect
CC       on a widely expressed receptor or ion channel found in both species
CC       (PubMed:33764053). At low doses (1 uM), the effects of the toxin are
CC       confined to a specific subpopulation of zebrafish and mouse DRG neurons
CC       (PubMed:33764053). In vivo, it induces long-lasting dramatic
CC       alterations in the locomotor behavior of zebrafish larvae
CC       (PubMed:33764053). It rapidly induces hypoactivity and death of larvae
CC       at high doses and it causes hyperactivity at lower doses
CC       (PubMed:33764053). In zebrafish adults, intramuscular injection causes
CC       the decrease of the movements and visited spaces (PubMed:33764053). In
CC       mice, intracranial injection causes lethargy and prolonges sleeping
CC       phases and reduced movement (PubMed:33764053).
CC       {ECO:0000269|PubMed:33764053}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:33764053}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:33764053}.
CC   -!- MISCELLANEOUS: The mature peptide does not contain cysteine residue.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the FARP (FMRFamide related peptide) family.
CC       {ECO:0000305}.
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Cleavage on pair of basic residues; Neurotoxin; Secreted;
KW   Signal; Toxin.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   PROPEP          20..25
FT                   /evidence="ECO:0000305|PubMed:33764053"
FT                   /id="PRO_0000453208"
FT   PEPTIDE         26..43
FT                   /note="Conorfamide-Ep1"
FT                   /evidence="ECO:0000305|PubMed:33764053"
FT                   /id="PRO_0000453209"
FT   PROPEP          45..59
FT                   /evidence="ECO:0000305|PubMed:33764053"
FT                   /id="PRO_0000453210"
FT   MOD_RES         43
FT                   /note="Isoleucine amide"
FT                   /evidence="ECO:0000305|PubMed:33764053"
SQ   SEQUENCE   59 AA;  6909 MW;  BE5FFF33D3605002 CRC64;
     MSGCGFLLLA LLLLVTVEAT KMEKKNFGIL FYFTRPRNNF VRIGRRDMQS PLLSERLRF