ID   CRFA1_CONSP             Reviewed;          12 AA.
AC   P58805;
DT   02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   02-MAY-2002, sequence version 1.
DT   22-APR-2020, entry version 45.
DE   RecName: Full=Conorfamide-Sr1 {ECO:0000305};
DE            Short=CNF-Sr1 {ECO:0000250|UniProtKB:P0DOZ7};
DE   AltName: Full=Cono-RFamide-Sr1 {ECO:0000303|PubMed:11738233};
OS   Conus spurius (Alphabet cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Lindaconus.
OX   NCBI_TaxID=192919;
RN   [1]
RP   PROTEIN SEQUENCE, SYNTHESIS, FUNCTION, BIOASSAY, MASS SPECTROMETRY,
RP   AMIDATION AT PHE-12, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=11738233; DOI=10.1016/s0041-0101(01)00176-3;
RA   Maillo M., Aguilar M.B., Lopez-Vera E., Craig A.G., Bulaj G., Olivera B.M.,
RA   Heimer de la Cotera E.P.;
RT   "Conorfamide, a Conus venom peptide belonging to the RFamide family of
RT   neuropeptides.";
RL   Toxicon 40:401-407(2002).
RN   [2]
RP   FUNCTION, AND SYNTHESIS.
RX   PubMed=30243794; DOI=10.1016/j.toxicon.2018.09.002;
RA   Espino S.S., Robinson S.D., Safavi-Hemami H., Gajewiak J., Yang W.,
RA   Olivera B.M., Liu Q.;
RT   "Conopeptides promote itch through human itch receptor hMgprX1.";
RL   Toxicon 154:28-34(2018).
CC   -!- FUNCTION: This peptide activates mouse sensory neuron-specific G-
CC       protein coupled receptors MRGPRX1, but not human receptors
CC       (PubMed:30243794). In vivo, causes hyperactivity in mice when injected
CC       intracranially (PubMed:11738233). {ECO:0000269|PubMed:11738233,
CC       ECO:0000269|PubMed:30243794}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11738233}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:11738233}.
CC   -!- MASS SPECTROMETRY: Mass=1454.8; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:11738233};
CC   -!- MISCELLANEOUS: The mature peptide does not contain cysteine residue.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the FARP (FMRFamide related peptide) family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   ConoServer; 1317; Conorfamide-Sr1.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing;
KW   G-protein coupled receptor impairing toxin; Neurotoxin; Secreted; Toxin.
FT   PEPTIDE         1..12
FT                   /note="Conorfamide-Sr1"
FT                   /evidence="ECO:0000269|PubMed:11738233"
FT                   /id="PRO_0000043716"
FT   MOD_RES         12
FT                   /note="Phenylalanine amide"
FT                   /evidence="ECO:0000269|PubMed:11738233"
SQ   SEQUENCE   12 AA;  1456 MW;  2510671E49D772D3 CRC64;
     GPMGWVPVFY RF