ID   CRFA2_CONSP             Reviewed;          12 AA.
AC   P85871;
DT   01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-2008, sequence version 1.
DT   25-MAY-2022, entry version 19.
DE   RecName: Full=Conorfamide-Sr2 {ECO:0000305};
DE            Short=CNF-Sr2 {ECO:0000250|UniProtKB:P0DOZ7};
DE   AltName: Full=Cono-RFamide-Sr2 {ECO:0000303|PubMed:18201803};
OS   Conus spurius (Alphabet cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Lindaconus.
OX   NCBI_TaxID=192919;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, BIOASSAY, SUBCELLULAR LOCATION, MASS
RP   SPECTROMETRY, GAMMA-CARBOXYGLUTAMATION AT GLU-4 AND GLU-8, AND AMIDATION AT
RP   ILE-12.
RC   TISSUE=Venom;
RX   PubMed=18201803; DOI=10.1016/j.peptides.2007.09.022;
RA   Aguilar M.B., Luna-Ramirez K.S., Echeverria D., Falcon A., Olivera B.M.,
RA   Heimer de la Cotera E.P., Maillo M.;
RT   "Conorfamide-Sr2, a gamma-carboxyglutamate-containing FMRFamide-related
RT   peptide from the venom of Conus spurius with activity in mice and
RT   mollusks.";
RL   Peptides 29:186-195(2008).
RN   [2]
RP   FUNCTION, AND SYNTHESIS.
RX   PubMed=30243794; DOI=10.1016/j.toxicon.2018.09.002;
RA   Espino S.S., Robinson S.D., Safavi-Hemami H., Gajewiak J., Yang W.,
RA   Olivera B.M., Liu Q.;
RT   "Conopeptides promote itch through human itch receptor hMgprX1.";
RL   Toxicon 154:28-34(2018).
CC   -!- FUNCTION: In contrast to other conorfamides, this peptide does not show
CC       activity on human and mouse sensory neuron-specific G-protein coupled
CC       receptors MRGPRX1 (PubMed:30243794). In vivo, it causes hyperactivity
CC       when injected intracranially into mice, and when injected into the foot
CC       of the fresh water snail P.paludosa (PubMed:18201803). It also causes
CC       paralysis when injected into the foot of the limpet P.opea
CC       (PubMed:18201803). Has no effect when injected below the rear part of
CC       the dorsal fin of the fish L.reticulatus (PubMed:18201803).
CC       {ECO:0000269|PubMed:18201803}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18201803}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:18201803}.
CC   -!- MASS SPECTROMETRY: Mass=1468.70; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:18201803};
CC   -!- MISCELLANEOUS: The mature peptide does not contain cysteine residue.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the FARP (FMRFamide related peptide) family.
CC       {ECO:0000305}.
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DR   ConoServer; 3538; Conorfamide-Sr2.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IDA:UniProtKB.
DR   GO; GO:0044616; P:modulation of relaxation of muscle in another organism; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Gamma-carboxyglutamic acid;
KW   Neurotoxin; Secreted; Toxin.
FT   PEPTIDE         1..12
FT                   /note="Conorfamide-Sr2"
FT                   /evidence="ECO:0000269|PubMed:18201803"
FT                   /id="PRO_0000343533"
FT   MOD_RES         4
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000269|PubMed:18201803"
FT   MOD_RES         8
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000269|PubMed:18201803"
FT   MOD_RES         12
FT                   /note="Isoleucine amide"
FT                   /evidence="ECO:0000269|PubMed:18201803"
SQ   SEQUENCE   12 AA;  1383 MW;  810A7C17C017376A CRC64;
     GPMEDPLEII RI