ID   CRFA3_CONSP             Reviewed;          15 AA.
AC   P0DM28;
DT   13-FEB-2019, integrated into UniProtKB/Swiss-Prot.
DT   13-FEB-2019, sequence version 1.
DT   23-FEB-2022, entry version 8.
DE   RecName: Full=Conorfamide-Sr3 {ECO:0000303|PubMed:28774677, ECO:0000303|PubMed:32823677};
DE            Short=CNF-Sr3 {ECO:0000303|PubMed:28774677, ECO:0000303|PubMed:32823677};
DE   AltName: Full=Cono-RFamide-Sr3 {ECO:0000303|PubMed:28774677};
OS   Conus spurius (Alphabet cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Lindaconus.
OX   NCBI_TaxID=192919;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SYNTHESIS, MASS SPECTROMETRY, AND AMIDATION AT
RP   PHE-15.
RC   TISSUE=Venom;
RX   PubMed=28774677; DOI=10.1016/j.toxicon.2017.07.024;
RA   Campos-Lira E., Carrillo E., Aguilar M.B., Gajewiak J., Gomez-Lagunas F.,
RA   Lopez-Vera E.;
RT   "Conorfamide-Sr3, a structurally novel specific inhibitor of the Shaker K+
RT   channel.";
RL   Toxicon 138:53-58(2017).
RN   [2]
RP   FUNCTION.
RX   PubMed=32823677; DOI=10.3390/md18080425;
RA   Lopez-Vera E., Martinez-Hernandez L., Aguilar M.B., Carrillo E.,
RA   Gajewiak J.;
RT   "Studies of conorfamide-Sr3 on human voltage-gated Kv1 potassium channel
RT   subtypes.";
RL   Mar. Drugs 18:0-0(2020).
CC   -!- FUNCTION: This peptide has moderate activity on some voltage-gated
CC       potassium channels. It reversibly inhibits the Shaker voltage-gated
CC       potassium channels (Kv) from drosophila (Kd=2.7 uM) (PubMed:28774677).
CC       In addition, it also irreversibly inhibits human Kv1.3/KCNA3 (IC(50)=24
CC       uM) and Kv1.6/KCNA6 (IC(50)=2.7 uM) potassium channels
CC       (PubMed:32823677). {ECO:0000269|PubMed:28774677,
CC       ECO:0000269|PubMed:32823677}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:28774677}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:28774677}.
CC   -!- MASS SPECTROMETRY: Mass=1726.77; Method=MALDI; Note=Monoisotopic mass.;
CC       Evidence={ECO:0000269|PubMed:28774677};
CC   -!- MISCELLANEOUS: Does not affect potassium channels from drosophila
CC       (Shab, Shaw, and Shal), as well as the human channel Kv10.1/KCNH1/EAG1
CC       (PubMed:28774677). Does not show activity on human Kv1.4/KCNA4 and
CC       Kv1.5/KCNA5 (PubMed:32823677). {ECO:0000269|PubMed:28774677,
CC       ECO:0000269|PubMed:32823677}.
CC   -!- MISCELLANEOUS: The mature peptide does not contain cysteine residue.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the FARP (FMRFamide related peptide) family.
CC       {ECO:0000305}.
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DR   ConoServer; 8699; Conorfamide-Sr3.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Ion channel impairing toxin;
KW   Potassium channel impairing toxin; Secreted; Toxin;
KW   Voltage-gated potassium channel impairing toxin.
FT   PEPTIDE         1..15
FT                   /note="Conorfamide-Sr3"
FT                   /evidence="ECO:0000269|PubMed:28774677"
FT                   /id="PRO_0000446232"
FT   MOD_RES         15
FT                   /note="Phenylalanine amide"
FT                   /evidence="ECO:0000269|PubMed:28774677"
SQ   SEQUENCE   15 AA;  1729 MW;  2510671E0F483336 CRC64;
     ATSGPMGWLP VFYRF