CRFC_ECOLI
ID CRFC_ECOLI Reviewed; 742 AA.
AC P0DM85; P16694; Q2M6J5;
DT 11-DEC-2013, integrated into UniProtKB/Swiss-Prot.
DT 11-DEC-2013, sequence version 1.
DT 03-AUG-2022, entry version 49.
DE RecName: Full=Clamp-binding protein CrfC;
DE AltName: Full=Clamp-binding sister replication fork colocalization protein;
GN Name=crfC; Synonyms=yjdA; OrderedLocusNames=b4109, JW4070;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT from 92.8 through 100 minutes.";
RL Nucleic Acids Res. 23:2105-2119(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION, LACK OF GTPASE, INTERACTION WITH DNAN, SUBUNIT, SUBCELLULAR
RP LOCATION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF GLN-23; GLN-41 AND
RP GLN-734.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=23994470; DOI=10.1016/j.celrep.2013.07.040;
RA Ozaki S., Matsuda Y., Keyamura K., Kawakami H., Noguchi Y., Kasho K.,
RA Nagata K., Masuda T., Sakiyama Y., Katayama T.;
RT "A replicase clamp-binding dynamin-like protein promotes colocalization of
RT nascent DNA strands and equipartitioning of chromosomes in E. coli.";
RL Cell Rep. 4:985-995(2013).
CC -!- FUNCTION: Important for the colocalization of sister nascent DNA
CC strands after replication fork passage during DNA replication, and for
CC positioning and subsequent partitioning of sister chromosomes. Does not
CC have GTPase activity on its own. {ECO:0000269|PubMed:23994470}.
CC -!- SUBUNIT: Forms homooligomers. Binds to the beta sliding clamp
CC processivity factor (DnaN) in the presence and absence of DNA, may bind
CC to the clamp itself as homodimers or trimers. Homooligomers may be able
CC to bind more than 1 clamp complex. {ECO:0000269|PubMed:23994470}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:23994470}.
CC Note=About half the protein co-localizes with beta sliding clamp (DnaN)
CC at midcell, the rest without clamp in quarter-cell positions when
CC chromosomes are condensed during DNA replication.
CC -!- DISRUPTION PHENOTYPE: A small proportion of anucleate cells, disrupts
CC nucleoid positioning, leads to premature separation of sister
CC replication forks. {ECO:0000269|PubMed:23994470}.
CC -!- SIMILARITY: Belongs to the TRAFAC class dynamin-like GTPase
CC superfamily. Dynamin/Fzo/YdjA family. {ECO:0000255|PROSITE-
CC ProRule:PRU01055}.
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DR EMBL; U14003; AAA97008.1; -; Genomic_DNA.
DR EMBL; U00096; AAC77070.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE78111.1; -; Genomic_DNA.
DR PIR; S56337; S56337.
DR RefSeq; NP_418533.1; NC_000913.3.
DR RefSeq; WP_000288603.1; NZ_LN832404.1.
DR AlphaFoldDB; P0DM85; -.
DR BioGRID; 4263087; 6.
DR BioGRID; 852912; 2.
DR IntAct; P0DM85; 13.
DR STRING; 511145.b4109; -.
DR PaxDb; P0DM85; -.
DR PRIDE; P0DM85; -.
DR EnsemblBacteria; AAC77070; AAC77070; b4109.
DR EnsemblBacteria; BAE78111; BAE78111; BAE78111.
DR GeneID; 948620; -.
DR KEGG; ecj:JW4070; -.
DR KEGG; eco:b4109; -.
DR PATRIC; fig|511145.12.peg.4240; -.
DR EchoBASE; EB1195; -.
DR eggNOG; COG0699; Bacteria.
DR HOGENOM; CLU_011600_2_0_6; -.
DR OMA; QRWVQDF; -.
DR BioCyc; EcoCyc:EG11210-MON; -.
DR PRO; PR:P0DM85; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0043590; C:bacterial nucleoid; IDA:EcoCyc.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:InterPro.
DR GO; GO:0003924; F:GTPase activity; IDA:EcoCyc.
DR GO; GO:0043022; F:ribosome binding; IBA:GO_Central.
DR GO; GO:0007059; P:chromosome segregation; IMP:EcoCyc.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:1900231; P:regulation of single-species biofilm formation on inanimate substrate; IMP:EcoCyc.
DR GO; GO:0000027; P:ribosomal large subunit assembly; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR045063; Dynamin_N.
DR InterPro; IPR030381; G_DYNAMIN_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00350; Dynamin_N; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51718; G_DYNAMIN_2; 1.
PE 1: Evidence at protein level;
KW Chromosome partition; Coiled coil; Cytoplasm; DNA replication;
KW Reference proteome.
FT CHAIN 1..742
FT /note="Clamp-binding protein CrfC"
FT /id="PRO_0000169728"
FT DOMAIN 66..402
FT /note="Dynamin-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT REGION 41..45
FT /note="Clamp-binding consensus"
FT REGION 76..83
FT /note="G1 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT REGION 102..104
FT /note="G2 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT REGION 236..239
FT /note="G3 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT REGION 297..300
FT /note="G4 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT REGION 331..334
FT /note="G5 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT COILED 440..472
FT /evidence="ECO:0000255"
FT MUTAGEN 23
FT /note="Q->A: Interacts with beta sliding clamp."
FT /evidence="ECO:0000269|PubMed:23994470"
FT MUTAGEN 41
FT /note="Q->A: No longer interacts with beta sliding clamp,
FT pattern of CrfC foci changes."
FT /evidence="ECO:0000269|PubMed:23994470"
FT MUTAGEN 734
FT /note="Q->A: Interacts with beta sliding clamp."
FT /evidence="ECO:0000269|PubMed:23994470"
SQ SEQUENCE 742 AA; 84371 MW; 8A7008FC16A026B3 CRC64;
MYTQTLYELS QEAERLLQLS RQQLQLLEKM PLSVPGDDAP QLALPWSQPN IAERHAMLNN
ELRKISRLEM VLAIVGTMKA GKSTTINAIV GTEVLPNRNR PMTALPTLIR HTPGQKEPVL
HFSHVAPIDC LIQQLQQRLR DCDIKHLTDV LEIDKDMRAL MQRIENGVAF EKYYLGAQPI
FHCLKSLNDL VRLAKALDVD FPFSAYAAIE HIPVIEVEFV HLAGLESYPG QLTLLDTPGP
NEAGQPHLQK MLNQQLARAS AVLAVLDYTQ LKSISDEEVR EAILAVGQSV PLYVLVNKFD
QQDRNSDDAD QVRALISGTL MKGCITPQQI FPVSSMWGYL ANRARYELAN NGKLPPPEQQ
RWVEDFAHAA LGRRWRHADL ADLEHIRHAA DQLWEDSLFA QPIQALLHAA YANASLYALR
SAAHKLLNYA QQAREYLDFR AHGLNVACEQ LRQNIHQIEE SLQLLQLNQA QVSGEIKHEI
ELALTSANHF LRQQQDALKV QLAALFQDDS EPLSEIRTRC ETLLQTAQNT ISRDFTLRFA
ELESTLCRVL TDVIRPIEQQ VKMELSESGF RPGFHFPVFH GVVPHFNTRQ LFSEVISRQE
ATDEQSTRLG VVRETFSRWL NQPDWGRGNE KSPTETVDYS VLQRALSAEV DLYCQQMAKV
LAEQVDESVT AGMNTFFAEF ASCLTELQTR LRESLALRQQ NESVVRLMQQ QLQQTVMTHG
WIYTDAQLLR DDIQTLFTAE RY
