CRF_MOUSE
ID CRF_MOUSE Reviewed; 187 AA.
AC Q8CIT0; Q14AA2;
DT 12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Corticoliberin;
DE AltName: Full=Corticotropin-releasing factor;
DE Short=CRF;
DE AltName: Full=Corticotropin-releasing hormone;
DE Flags: Precursor;
GN Name=Crh; Synonyms=Gm1347;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RC STRAIN=BALB/cJ;
RX PubMed=19912808; DOI=10.1016/1044-7431(91)90054-r;
RA Seasholtz A.F., Bourbonais F.J., Harnden C.E., Camper S.A.;
RT "Nucleotide sequence and expression of the mouse corticotropin-releasing
RT hormone gene.";
RL Mol. Cell. Neurosci. 2:266-273(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, AND INDUCTION BY STRESS.
RX PubMed=23470617; DOI=10.1053/j.gastro.2013.02.038;
RA Sun Y., Zhang M., Chen C.C., Gillilland M. III, Sun X., El-Zaatari M.,
RA Huffnagle G.B., Young V.B., Zhang J., Hong S.C., Chang Y.M., Gumucio D.L.,
RA Owyang C., Kao J.Y.;
RT "Stress-induced corticotropin-releasing hormone-mediated NLRP6 inflammasome
RT inhibition and transmissible enteritis in mice.";
RL Gastroenterology 144:1478-1487(2013).
CC -!- FUNCTION: Hormone regulating the release of corticotropin from
CC pituitary gland. Induces NLRP6 in intestinal epithelial cells, hence
CC may influence gut microbiota profile (PubMed:23470617).
CC {ECO:0000250|UniProtKB:P06296, ECO:0000269|PubMed:23470617}.
CC -!- SUBUNIT: Interacts (via C-terminus) with CRFR1 (via N-terminal
CC extracellular domain). {ECO:0000250|UniProtKB:P06850}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P06296}.
CC -!- TISSUE SPECIFICITY: Expressed in parvocellular paraventricular nucleus
CC of the hypothalamus and in medial accessory olivary nucleus.
CC {ECO:0000269|PubMed:19912808}.
CC -!- INDUCTION: Up-regulated during water-avoidance stress.
CC {ECO:0000269|PubMed:23470617}.
CC -!- SIMILARITY: Belongs to the sauvagine/corticotropin-releasing
CC factor/urotensin I family. {ECO:0000305}.
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DR EMBL; AY128673; AAN07905.1; -; Genomic_DNA.
DR EMBL; BC116921; AAI16922.1; -; mRNA.
DR EMBL; BC119036; AAI19037.1; -; mRNA.
DR CCDS; CCDS17258.1; -.
DR RefSeq; NP_991338.1; NM_205769.3.
DR AlphaFoldDB; Q8CIT0; -.
DR STRING; 10090.ENSMUSP00000061185; -.
DR PhosphoSitePlus; Q8CIT0; -.
DR PaxDb; Q8CIT0; -.
DR PRIDE; Q8CIT0; -.
DR ProteomicsDB; 285308; -.
DR Antibodypedia; 11984; 555 antibodies from 39 providers.
DR DNASU; 12918; -.
DR Ensembl; ENSMUST00000061294; ENSMUSP00000061185; ENSMUSG00000049796.
DR GeneID; 12918; -.
DR KEGG; mmu:12918; -.
DR UCSC; uc008orx.2; mouse.
DR CTD; 1392; -.
DR MGI; MGI:88496; Crh.
DR VEuPathDB; HostDB:ENSMUSG00000049796; -.
DR eggNOG; ENOG502S25G; Eukaryota.
DR GeneTree; ENSGT00940000154473; -.
DR HOGENOM; CLU_136288_0_0_1; -.
DR InParanoid; Q8CIT0; -.
DR OMA; HNNRKLM; -.
DR OrthoDB; 1532326at2759; -.
DR PhylomeDB; Q8CIT0; -.
DR TreeFam; TF332956; -.
DR Reactome; R-MMU-373080; Class B/2 (Secretin family receptors).
DR Reactome; R-MMU-418555; G alpha (s) signalling events.
DR BioGRID-ORCS; 12918; 4 hits in 74 CRISPR screens.
DR PRO; PR:Q8CIT0; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q8CIT0; protein.
DR Bgee; ENSMUSG00000049796; Expressed in medial vestibular nucleus and 67 other tissues.
DR Genevisible; Q8CIT0; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR GO; GO:0005615; C:extracellular space; ISO:MGI.
DR GO; GO:0043025; C:neuronal cell body; IDA:MGI.
DR GO; GO:0043204; C:perikaryon; ISO:MGI.
DR GO; GO:0045202; C:synapse; IEA:GOC.
DR GO; GO:0043196; C:varicosity; ISO:MGI.
DR GO; GO:0017045; F:corticotropin-releasing hormone activity; IBA:GO_Central.
DR GO; GO:0051430; F:corticotropin-releasing hormone receptor 1 binding; ISO:MGI.
DR GO; GO:0051431; F:corticotropin-releasing hormone receptor 2 binding; ISO:MGI.
DR GO; GO:0030325; P:adrenal gland development; IMP:MGI.
DR GO; GO:0008306; P:associative learning; ISO:MGI.
DR GO; GO:0071314; P:cellular response to cocaine; ISO:MGI.
DR GO; GO:0071549; P:cellular response to dexamethasone stimulus; IEA:Ensembl.
DR GO; GO:0016101; P:diterpenoid metabolic process; IEA:Ensembl.
DR GO; GO:0007565; P:female pregnancy; ISO:MGI.
DR GO; GO:0006704; P:glucocorticoid biosynthetic process; IMP:MGI.
DR GO; GO:0008628; P:hormone-mediated apoptotic signaling pathway; ISO:MGI.
DR GO; GO:0021854; P:hypothalamus development; IEA:Ensembl.
DR GO; GO:0006954; P:inflammatory response; IMP:MGI.
DR GO; GO:0050801; P:ion homeostasis; IEA:Ensembl.
DR GO; GO:0007611; P:learning or memory; ISO:MGI.
DR GO; GO:0035641; P:locomotory exploration behavior; IGI:MGI.
DR GO; GO:0060291; P:long-term synaptic potentiation; ISO:MGI.
DR GO; GO:0030324; P:lung development; IMP:MGI.
DR GO; GO:0060548; P:negative regulation of cell death; ISO:MGI.
DR GO; GO:0032811; P:negative regulation of epinephrine secretion; ISO:MGI.
DR GO; GO:0010629; P:negative regulation of gene expression; ISO:MGI.
DR GO; GO:0070093; P:negative regulation of glucagon secretion; ISO:MGI.
DR GO; GO:0033685; P:negative regulation of luteinizing hormone secretion; ISO:MGI.
DR GO; GO:0010700; P:negative regulation of norepinephrine secretion; ISO:MGI.
DR GO; GO:0003085; P:negative regulation of systemic arterial blood pressure; ISO:MGI.
DR GO; GO:2000987; P:positive regulation of behavioral fear response; ISO:MGI.
DR GO; GO:0090280; P:positive regulation of calcium ion import; ISO:MGI.
DR GO; GO:0043950; P:positive regulation of cAMP-mediated signaling; ISO:MGI.
DR GO; GO:0010942; P:positive regulation of cell death; ISO:MGI.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:MGI.
DR GO; GO:2000854; P:positive regulation of corticosterone secretion; ISO:MGI.
DR GO; GO:0051461; P:positive regulation of corticotropin secretion; ISO:MGI.
DR GO; GO:0051464; P:positive regulation of cortisol secretion; ISO:MGI.
DR GO; GO:0060456; P:positive regulation of digestive system process; ISO:MGI.
DR GO; GO:0010628; P:positive regulation of gene expression; ISO:MGI.
DR GO; GO:0035774; P:positive regulation of insulin secretion involved in cellular response to glucose stimulus; ISO:MGI.
DR GO; GO:0001934; P:positive regulation of protein phosphorylation; ISO:MGI.
DR GO; GO:2000310; P:regulation of NMDA receptor activity; ISO:MGI.
DR GO; GO:0014062; P:regulation of serotonin secretion; ISO:MGI.
DR GO; GO:0051412; P:response to corticosterone; IEA:Ensembl.
DR GO; GO:0043627; P:response to estrogen; IEA:Ensembl.
DR GO; GO:0045471; P:response to ethanol; IEA:Ensembl.
DR GO; GO:0045472; P:response to ether; IEA:Ensembl.
DR GO; GO:0035902; P:response to immobilization stress; IEA:Ensembl.
DR GO; GO:0048265; P:response to pain; IEA:Ensembl.
DR GO; GO:0009410; P:response to xenobiotic stimulus; IEA:Ensembl.
DR GO; GO:0001963; P:synaptic transmission, dopaminergic; ISO:MGI.
DR InterPro; IPR018446; Corticotropin-releasing_fac_CS.
DR InterPro; IPR000187; CRF.
DR InterPro; IPR003620; Urocortin_CRF.
DR PANTHER; PTHR15035; PTHR15035; 1.
DR Pfam; PF00473; CRF; 1.
DR PRINTS; PR01612; CRFFAMILY.
DR SMART; SM00039; CRF; 1.
DR PROSITE; PS00511; CRF; 1.
PE 2: Evidence at transcript level;
KW Amidation; Cleavage on pair of basic residues; Hormone; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..144
FT /evidence="ECO:0000250"
FT /id="PRO_0000006214"
FT PEPTIDE 145..185
FT /note="Corticoliberin"
FT /id="PRO_0000006215"
FT REGION 75..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 114..146
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 75..90
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 119..146
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 185
FT /note="Isoleucine amide"
FT /evidence="ECO:0000250"
SQ SEQUENCE 187 AA; 20778 MW; 5E51665291FAA998 CRC64;
MRLRLLVSAG MLLVALSSCL PCRALLSRGS VPRAPRAPQP LNFLQPEQPQ QPQPVLIRMG
EEYFLRLGNL NRSPAARLSP NSTPLTAGRG SRPSHDQAAA NFFRVLLQQL QMPQRSLDSR
AEPAERGAED ALGGHQGALE RERRSEEPPI SLDLTFHLLR EVLEMARAEQ LAQQAHSNRK
LMEIIGK
