CRF_RAT
ID CRF_RAT Reviewed; 187 AA.
AC P01143;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1988, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Corticoliberin;
DE AltName: Full=Corticotropin-releasing factor;
DE Short=CRF;
DE AltName: Full=Corticotropin-releasing hormone;
DE Flags: Precursor;
GN Name=Crh;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=3876950; DOI=10.1016/0014-5793(85)80994-7;
RA Jingami H., Mizuno N., Takahashi H., Shibahara S., Furutani Y., Imura H.,
RA Numa S.;
RT "Cloning and sequence analysis of cDNA for rat corticotropin-releasing
RT factor precursor.";
RL FEBS Lett. 191:63-66(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Liver;
RX PubMed=3274895; DOI=10.1210/mend-1-5-363;
RA Thompson R.C., Seasholtz A.F., Herbert E.;
RT "Rat corticotropin-releasing hormone gene: sequence and tissue-specific
RT expression.";
RL Mol. Endocrinol. 1:363-370(1987).
RN [3]
RP PROTEIN SEQUENCE OF 145-185, FUNCTION, AMIDATION AT ILE-185, AND
RP SUBCELLULAR LOCATION.
RX PubMed=6603620; DOI=10.1073/pnas.80.15.4851;
RA Rivier J., Spiess J., Vale W.;
RT "Characterization of rat hypothalamic corticotropin-releasing factor.";
RL Proc. Natl. Acad. Sci. U.S.A. 80:4851-4855(1983).
CC -!- FUNCTION: Hormone regulating the release of corticotropin from
CC pituitary gland (PubMed:6603620). Induces NLRP6 in intestinal
CC epithelial cells, hence may influence gut microbiota profile (By
CC similarity). {ECO:0000250|UniProtKB:Q8CIT0,
CC ECO:0000269|PubMed:6603620}.
CC -!- SUBUNIT: Interacts (via C-terminus) with CRFR1 (via N-terminal
CC extracellular domain). {ECO:0000250|UniProtKB:P06850}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:6603620}.
CC -!- TISSUE SPECIFICITY: Produced by the hypothalamus.
CC {ECO:0000269|PubMed:3876950}.
CC -!- SIMILARITY: Belongs to the sauvagine/corticotropin-releasing
CC factor/urotensin I family. {ECO:0000305}.
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DR EMBL; X03036; CAA26838.1; -; mRNA.
DR EMBL; M54987; AAA40965.1; -; Genomic_DNA.
DR PIR; A40906; RHRTCE.
DR RefSeq; NP_112281.1; NM_031019.1.
DR AlphaFoldDB; P01143; -.
DR STRING; 10116.ENSRNOP00000016953; -.
DR BindingDB; P01143; -.
DR PaxDb; P01143; -.
DR Ensembl; ENSRNOT00000016953; ENSRNOP00000016953; ENSRNOG00000012703.
DR GeneID; 81648; -.
DR KEGG; rno:81648; -.
DR UCSC; RGD:620505; rat.
DR CTD; 1392; -.
DR RGD; 620505; Crh.
DR eggNOG; ENOG502S25G; Eukaryota.
DR GeneTree; ENSGT00940000154473; -.
DR HOGENOM; CLU_136288_0_0_1; -.
DR InParanoid; P01143; -.
DR OMA; HNNRKLM; -.
DR OrthoDB; 1532326at2759; -.
DR PhylomeDB; P01143; -.
DR TreeFam; TF332956; -.
DR Reactome; R-RNO-373080; Class B/2 (Secretin family receptors).
DR PRO; PR:P01143; -.
DR Proteomes; UP000002494; Chromosome 2.
DR Bgee; ENSRNOG00000012703; Expressed in cerebral cortex and 2 other tissues.
DR Genevisible; P01143; RN.
DR GO; GO:0005737; C:cytoplasm; IDA:RGD.
DR GO; GO:0005615; C:extracellular space; IDA:RGD.
DR GO; GO:0043025; C:neuronal cell body; IDA:RGD.
DR GO; GO:0043204; C:perikaryon; IDA:RGD.
DR GO; GO:0045202; C:synapse; IEA:GOC.
DR GO; GO:0043196; C:varicosity; IDA:RGD.
DR GO; GO:0017045; F:corticotropin-releasing hormone activity; IBA:GO_Central.
DR GO; GO:0051430; F:corticotropin-releasing hormone receptor 1 binding; IDA:RGD.
DR GO; GO:0051431; F:corticotropin-releasing hormone receptor 2 binding; IDA:RGD.
DR GO; GO:0030325; P:adrenal gland development; ISO:RGD.
DR GO; GO:0008306; P:associative learning; IDA:RGD.
DR GO; GO:0071314; P:cellular response to cocaine; IDA:UniProtKB.
DR GO; GO:0071549; P:cellular response to dexamethasone stimulus; IEP:RGD.
DR GO; GO:0016101; P:diterpenoid metabolic process; IEP:RGD.
DR GO; GO:0007565; P:female pregnancy; ISO:RGD.
DR GO; GO:0006704; P:glucocorticoid biosynthetic process; ISO:RGD.
DR GO; GO:0008628; P:hormone-mediated apoptotic signaling pathway; IDA:RGD.
DR GO; GO:0021854; P:hypothalamus development; IEP:RGD.
DR GO; GO:0006954; P:inflammatory response; ISO:RGD.
DR GO; GO:0050801; P:ion homeostasis; IEP:RGD.
DR GO; GO:0007611; P:learning or memory; IMP:RGD.
DR GO; GO:0035641; P:locomotory exploration behavior; ISO:RGD.
DR GO; GO:0060291; P:long-term synaptic potentiation; IDA:RGD.
DR GO; GO:0030324; P:lung development; ISO:RGD.
DR GO; GO:0060548; P:negative regulation of cell death; IDA:RGD.
DR GO; GO:0032811; P:negative regulation of epinephrine secretion; IDA:RGD.
DR GO; GO:0010629; P:negative regulation of gene expression; IMP:RGD.
DR GO; GO:0070093; P:negative regulation of glucagon secretion; IDA:RGD.
DR GO; GO:0033685; P:negative regulation of luteinizing hormone secretion; IMP:RGD.
DR GO; GO:0010700; P:negative regulation of norepinephrine secretion; IDA:RGD.
DR GO; GO:0003085; P:negative regulation of systemic arterial blood pressure; IDA:RGD.
DR GO; GO:2000987; P:positive regulation of behavioral fear response; IDA:RGD.
DR GO; GO:0090280; P:positive regulation of calcium ion import; IDA:RGD.
DR GO; GO:0043950; P:positive regulation of cAMP-mediated signaling; IDA:RGD.
DR GO; GO:0010942; P:positive regulation of cell death; IDA:RGD.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IDA:RGD.
DR GO; GO:2000854; P:positive regulation of corticosterone secretion; IDA:RGD.
DR GO; GO:0051461; P:positive regulation of corticotropin secretion; IDA:RGD.
DR GO; GO:0051464; P:positive regulation of cortisol secretion; ISO:RGD.
DR GO; GO:0060456; P:positive regulation of digestive system process; IDA:RGD.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:RGD.
DR GO; GO:0035774; P:positive regulation of insulin secretion involved in cellular response to glucose stimulus; IDA:RGD.
DR GO; GO:0001934; P:positive regulation of protein phosphorylation; IDA:RGD.
DR GO; GO:2000310; P:regulation of NMDA receptor activity; IDA:UniProtKB.
DR GO; GO:0014062; P:regulation of serotonin secretion; IDA:RGD.
DR GO; GO:0042220; P:response to cocaine; IEP:RGD.
DR GO; GO:0051412; P:response to corticosterone; IEP:RGD.
DR GO; GO:0043627; P:response to estrogen; IEP:RGD.
DR GO; GO:0045471; P:response to ethanol; IEP:RGD.
DR GO; GO:0045472; P:response to ether; IEP:RGD.
DR GO; GO:0035902; P:response to immobilization stress; IEP:RGD.
DR GO; GO:0048265; P:response to pain; IEP:RGD.
DR GO; GO:0009410; P:response to xenobiotic stimulus; IEP:RGD.
DR GO; GO:0008202; P:steroid metabolic process; IEP:RGD.
DR GO; GO:0001963; P:synaptic transmission, dopaminergic; IDA:UniProtKB.
DR InterPro; IPR018446; Corticotropin-releasing_fac_CS.
DR InterPro; IPR000187; CRF.
DR InterPro; IPR003620; Urocortin_CRF.
DR PANTHER; PTHR15035; PTHR15035; 1.
DR Pfam; PF00473; CRF; 1.
DR PRINTS; PR01612; CRFFAMILY.
DR SMART; SM00039; CRF; 1.
DR PROSITE; PS00511; CRF; 1.
PE 1: Evidence at protein level;
KW Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW Hormone; Reference proteome; Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT PROPEP 25..144
FT /evidence="ECO:0000269|PubMed:6603620"
FT /id="PRO_0000006218"
FT PEPTIDE 145..185
FT /note="Corticoliberin"
FT /evidence="ECO:0000269|PubMed:6603620"
FT /id="PRO_0000006219"
FT REGION 75..95
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 75..90
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 185
FT /note="Isoleucine amide"
FT /evidence="ECO:0000269|PubMed:6603620"
SQ SEQUENCE 187 AA; 20680 MW; 911602C82A444CFB CRC64;
MRLRLLVSAG MLLVALSPCL PCRALLSRGS VSGAPRAPQP LNFLQPEQPQ QPQPILIRMG
EEYFLRLGNL NRSPAARLSP NSTPLTAGRG SRPSHDQAAA NFFRVLLQQL QMPQRPLDSS
TELAERGAED ALGGHQGALE RERRSEEPPI SLDLTFHLLR EVLEMARAEQ LAQQAHSNRK
LMEIIGK
