CRGE_MOUSE
ID CRGE_MOUSE Reviewed; 174 AA.
AC Q03740; O89028; P26999; Q9CXK5;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 4.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Gamma-crystallin E;
DE AltName: Full=Gamma-E-crystallin;
GN Name=Cryge;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=6096855; DOI=10.1073/pnas.81.24.7762;
RA Breitman M.L., Lok S., Wistow G., Piatigorsky J., Treton J.A., Gold R.J.M.,
RA Tsui L.-C.;
RT "Gamma-crystallin family of the mouse lens: structural and evolutionary
RT relationships.";
RL Proc. Natl. Acad. Sci. U.S.A. 81:7762-7766(1984).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=(101/E1XC3H/E1)F1; TISSUE=Liver;
RX PubMed=1916296; DOI=10.1016/0378-1119(91)90260-i;
RA Graw J., Coban L., Liebstein A., Werner T.;
RT "Murine gamma E-crystallin is distinct from murine gamma 2-crystallin.";
RL Gene 104:265-270(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryonic head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=27517863; DOI=10.1371/journal.pone.0161140;
RA Hartwich H., Rosengauer E., Ruettiger L., Wilms V., Waterholter S.K.,
RA Nothwang H.G.;
RT "Functional Role of gamma-Crystallin N in the Auditory Hindbrain.";
RL PLoS ONE 11:E0161140-E0161140(2016).
CC -!- FUNCTION: Crystallins are the dominant structural components of the
CC vertebrate eye lens.
CC -!- TISSUE SPECIFICITY: Detected in the superior olivary complex of the
CC auditory hindbrain. {ECO:0000269|PubMed:27517863}.
CC -!- DEVELOPMENTAL STAGE: In the embryo, expressed by day 12 of gestation.
CC Maximum levels are found at day 30-40 followed by a rapid decline.
CC -!- DOMAIN: Has a two-domain beta-structure, folded into four very similar
CC Greek key motifs.
CC -!- MISCELLANEOUS: There are six different gamma crystallins identified in
CC mouse lens.
CC -!- SIMILARITY: Belongs to the beta/gamma-crystallin family. {ECO:0000305}.
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DR EMBL; K02584; AAA03228.1; -; mRNA.
DR EMBL; X57855; CAA40990.1; -; Genomic_DNA.
DR EMBL; AK014301; BAB29256.1; -; mRNA.
DR EMBL; BC078417; AAH78417.1; -; mRNA.
DR CCDS; CCDS35598.1; -.
DR PIR; A02931; CYMSG2.
DR PIR; JS0596; JS0596.
DR PIR; S26811; S26811.
DR RefSeq; NP_031803.3; NM_007777.3.
DR AlphaFoldDB; Q03740; -.
DR SMR; Q03740; -.
DR BioGRID; 198920; 2.
DR STRING; 10090.ENSMUSP00000084617; -.
DR PaxDb; Q03740; -.
DR PRIDE; Q03740; -.
DR ProteomicsDB; 283955; -.
DR DNASU; 12968; -.
DR Ensembl; ENSMUST00000087359; ENSMUSP00000084617; ENSMUSG00000070870.
DR GeneID; 12968; -.
DR KEGG; mmu:12968; -.
DR UCSC; uc007bhf.1; mouse.
DR CTD; 12968; -.
DR MGI; MGI:88525; Cryge.
DR VEuPathDB; HostDB:ENSMUSG00000070870; -.
DR eggNOG; ENOG502RXJY; Eukaryota.
DR GeneTree; ENSGT00940000163272; -.
DR HOGENOM; CLU_081883_1_1_1; -.
DR InParanoid; Q03740; -.
DR OMA; SECSDLH; -.
DR OrthoDB; 1220704at2759; -.
DR PhylomeDB; Q03740; -.
DR BioGRID-ORCS; 12968; 2 hits in 38 CRISPR screens.
DR ChiTaRS; Cryge; mouse.
DR PRO; PR:Q03740; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q03740; protein.
DR Bgee; ENSMUSG00000070870; Expressed in lens of camera-type eye and 31 other tissues.
DR ExpressionAtlas; Q03740; baseline and differential.
DR GO; GO:0005212; F:structural constituent of eye lens; IBA:GO_Central.
DR GO; GO:0034614; P:cellular response to reactive oxygen species; ISO:MGI.
DR GO; GO:0001654; P:eye development; IMP:MGI.
DR GO; GO:0002088; P:lens development in camera-type eye; IMP:MGI.
DR GO; GO:0007601; P:visual perception; ISO:MGI.
DR InterPro; IPR001064; Beta/gamma_crystallin.
DR InterPro; IPR011024; G_crystallin-like.
DR Pfam; PF00030; Crystall; 2.
DR PRINTS; PR01367; BGCRYSTALLIN.
DR SMART; SM00247; XTALbg; 2.
DR SUPFAM; SSF49695; SSF49695; 1.
DR PROSITE; PS50915; CRYSTALLIN_BETA_GAMMA; 4.
PE 2: Evidence at transcript level;
KW Eye lens protein; Reference proteome; Repeat.
FT CHAIN 1..174
FT /note="Gamma-crystallin E"
FT /id="PRO_0000057599"
FT DOMAIN 2..40
FT /note="Beta/gamma crystallin 'Greek key' 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT DOMAIN 41..83
FT /note="Beta/gamma crystallin 'Greek key' 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT DOMAIN 88..128
FT /note="Beta/gamma crystallin 'Greek key' 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT DOMAIN 129..171
FT /note="Beta/gamma crystallin 'Greek key' 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT REGION 84..87
FT /note="Connecting peptide"
FT CONFLICT 47
FT /note="E -> Q (in Ref. 2; CAA40990)"
FT /evidence="ECO:0000305"
FT CONFLICT 52
FT /note="A -> T (in Ref. 1; AAA03228 and 2; CAA40990)"
FT /evidence="ECO:0000305"
FT CONFLICT 91
FT /note="K -> R (in Ref. 1; AAA03228)"
FT /evidence="ECO:0000305"
FT CONFLICT 110
FT /note="S -> P (in Ref. 1; AAA03228)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 174 AA; 21196 MW; FBD27BC163B4C947 CRC64;
MGKITFYEDR GFQGRHYECS TDHSNLQPYF SRCNSVRVDS GCWMLYEQPN FAGCQYFLRR
GDYPDYQQWM GFSDSVRSCR LIPHSSSHRI KIYEREDYRG QMVEITDDCS HLQDRFHFSD
FHSFHVMEGY WVLYEMPNYR GRQYLLRPGE YRRYHDWGAM NARVGSLRRI MDFY
