CRGE_RAT
ID CRGE_RAT Reviewed; 174 AA.
AC P02528;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 25-MAY-2022, entry version 155.
DE RecName: Full=Gamma-crystallin E;
DE AltName: Full=Gamma-2;
DE AltName: Full=Gamma-E-crystallin;
DE AltName: Full=Gamma-crystallin 3-1;
GN Name=Cryge;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=6294661; DOI=10.1073/pnas.79.22.6876;
RA Moormann R.J.M., den Dunnen J.T., Bloemendal H., Schoenmakers J.G.G.;
RT "Extensive intragenic sequence homology in two distinct rat lens gamma-
RT crystallin cDNAs suggests duplications of a primordial gene.";
RL Proc. Natl. Acad. Sci. U.S.A. 79:6876-6880(1982).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2777080; DOI=10.1016/0378-1119(89)90223-0;
RA den Dunnen J.T., van Neck J.W., Cremers F.P.M., Lubsen N.H.,
RA Schoenmakers J.G.G.;
RT "Nucleotide sequence of the rat gamma-crystallin gene region and comparison
RT with an orthologous human region.";
RL Gene 78:201-213(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6319707; DOI=10.1016/0022-2836(83)90034-7;
RA Moormann R.J.M., den Dunnen J.T., Mulleners L., Andreoli P., Bloemendal H.,
RA Schoenmakers J.G.G.;
RT "Strict co-linearity of genetic and protein folding domains in an
RT intragenically duplicated rat lens gamma-crystallin gene.";
RL J. Mol. Biol. 171:353-368(1983).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=27517863; DOI=10.1371/journal.pone.0161140;
RA Hartwich H., Rosengauer E., Ruettiger L., Wilms V., Waterholter S.K.,
RA Nothwang H.G.;
RT "Functional Role of gamma-Crystallin N in the Auditory Hindbrain.";
RL PLoS ONE 11:E0161140-E0161140(2016).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
RC TISSUE=Lens;
RX PubMed=9367641; DOI=10.1006/exer.1997.0368;
RA Norledge B.V., Hay R.E., Bateman O.A., Slingsby C., Driessen H.P.C.;
RT "Towards a molecular understanding of phase separation in the lens: a
RT comparison of the X-ray structures of two high Tc gamma-crystallins, gammaE
RT and gammaF, with two low Tc gamma-crystallins, gammaB and gammaD.";
RL Exp. Eye Res. 65:609-630(1997).
CC -!- FUNCTION: Crystallins are the dominant structural components of the
CC vertebrate eye lens.
CC -!- TISSUE SPECIFICITY: Detected in the superior olivary complex and fibers
CC of the ventral aoustic stria of the auditory hindbrain.
CC {ECO:0000269|PubMed:27517863}.
CC -!- DOMAIN: Has a two-domain beta-structure, folded into four very similar
CC Greek key motifs.
CC -!- MISCELLANEOUS: There are six different gamma crystallins identified in
CC rat lens.
CC -!- SIMILARITY: Belongs to the beta/gamma-crystallin family. {ECO:0000305}.
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DR EMBL; M19359; AAA40985.1; -; Genomic_DNA.
DR EMBL; J00716; AAA40987.1; -; mRNA.
DR EMBL; X00271; CAA25073.1; -; Genomic_DNA.
DR PIR; A02930; CYRTG1.
DR PIR; I49617; I49617.
DR PIR; I56381; I56381.
DR RefSeq; NP_775411.1; NM_173289.1.
DR PDB; 1A5D; X-ray; 2.30 A; A/B=2-174.
DR PDB; 1ZGT; X-ray; 1.45 A; A=2-174.
DR PDB; 1ZIE; X-ray; 1.44 A; A=2-174.
DR PDB; 1ZIQ; X-ray; 1.72 A; A=2-174.
DR PDB; 1ZIR; X-ray; 1.36 A; A=2-174.
DR PDBsum; 1A5D; -.
DR PDBsum; 1ZGT; -.
DR PDBsum; 1ZIE; -.
DR PDBsum; 1ZIQ; -.
DR PDBsum; 1ZIR; -.
DR AlphaFoldDB; P02528; -.
DR SMR; P02528; -.
DR GeneID; 24279; -.
DR KEGG; rno:24279; -.
DR UCSC; RGD:2423; rat.
DR CTD; 12968; -.
DR RGD; 2423; Cryge.
DR InParanoid; P02528; -.
DR OrthoDB; 954003at2759; -.
DR PhylomeDB; P02528; -.
DR EvolutionaryTrace; P02528; -.
DR PRO; PR:P02528; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005212; F:structural constituent of eye lens; IBA:GO_Central.
DR GO; GO:0002088; P:lens development in camera-type eye; IEP:RGD.
DR GO; GO:0007601; P:visual perception; IBA:GO_Central.
DR InterPro; IPR001064; Beta/gamma_crystallin.
DR InterPro; IPR011024; G_crystallin-like.
DR Pfam; PF00030; Crystall; 2.
DR PRINTS; PR01367; BGCRYSTALLIN.
DR SMART; SM00247; XTALbg; 2.
DR SUPFAM; SSF49695; SSF49695; 1.
DR PROSITE; PS50915; CRYSTALLIN_BETA_GAMMA; 4.
PE 1: Evidence at protein level;
KW 3D-structure; Eye lens protein; Reference proteome; Repeat.
FT CHAIN 1..174
FT /note="Gamma-crystallin E"
FT /id="PRO_0000057593"
FT DOMAIN 2..40
FT /note="Beta/gamma crystallin 'Greek key' 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT DOMAIN 41..83
FT /note="Beta/gamma crystallin 'Greek key' 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT DOMAIN 88..128
FT /note="Beta/gamma crystallin 'Greek key' 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT DOMAIN 129..171
FT /note="Beta/gamma crystallin 'Greek key' 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT REGION 84..87
FT /note="Connecting peptide"
FT STRAND 3..9
FT /evidence="ECO:0007829|PDB:1ZIR"
FT HELIX 10..12
FT /evidence="ECO:0007829|PDB:1ZIR"
FT STRAND 13..19
FT /evidence="ECO:0007829|PDB:1ZIR"
FT TURN 27..29
FT /evidence="ECO:0007829|PDB:1ZIR"
FT STRAND 34..48
FT /evidence="ECO:0007829|PDB:1ZIR"
FT TURN 49..51
FT /evidence="ECO:0007829|PDB:1ZIR"
FT STRAND 52..58
FT /evidence="ECO:0007829|PDB:1ZIR"
FT STRAND 60..65
FT /evidence="ECO:0007829|PDB:1ZIR"
FT HELIX 66..69
FT /evidence="ECO:0007829|PDB:1ZIR"
FT STRAND 72..74
FT /evidence="ECO:0007829|PDB:1ZIR"
FT STRAND 78..82
FT /evidence="ECO:0007829|PDB:1ZIR"
FT STRAND 89..95
FT /evidence="ECO:0007829|PDB:1ZIR"
FT HELIX 96..98
FT /evidence="ECO:0007829|PDB:1ZIR"
FT STRAND 99..107
FT /evidence="ECO:0007829|PDB:1ZIR"
FT HELIX 112..115
FT /evidence="ECO:0007829|PDB:1ZIR"
FT STRAND 123..129
FT /evidence="ECO:0007829|PDB:1ZIR"
FT STRAND 131..136
FT /evidence="ECO:0007829|PDB:1ZIR"
FT TURN 137..139
FT /evidence="ECO:0007829|PDB:1ZIR"
FT STRAND 140..146
FT /evidence="ECO:0007829|PDB:1ZIR"
FT STRAND 148..151
FT /evidence="ECO:0007829|PDB:1ZIR"
FT HELIX 154..157
FT /evidence="ECO:0007829|PDB:1ZIR"
FT STRAND 166..169
FT /evidence="ECO:0007829|PDB:1ZIR"
SQ SEQUENCE 174 AA; 21264 MW; 8DF9A2A101232BE2 CRC64;
MGKITFYEDR GFQGRHYECS TDHSNLQPYF SRCNSVRVDS GCWMLYEQPN FTGCQYFLRR
GDYPDYQQWM GFSDSVRSCR LIPHSSSHRI RIYEREDYRG QMVEITDDCP HLQDRFHFSD
FHSFHVMEGY WVLYEMPNYR GRQYLLRPGE YRRYHDWGAM NARVGSLRRI MDFY
