CRGF_MOUSE
ID CRGF_MOUSE Reviewed; 174 AA.
AC Q9CXV3;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Gamma-crystallin F;
DE AltName: Full=Gamma-F-crystallin;
GN Name=Crygf;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-160.
RC STRAIN=CD-1; TISSUE=Eye;
RX PubMed=1623964; DOI=10.1016/0014-4835(92)90034-p;
RA Goring D.R., Breitman M.L., Tsui L.-C.;
RT "Temporal regulation of six crystallin transcripts during mouse lens
RT development.";
RL Exp. Eye Res. 54:785-795(1992).
RN [4]
RP NUCLEOTIDE SEQUENCE OF 1-69.
RC TISSUE=Lens;
RX PubMed=3837188; DOI=10.1128/mcb.5.9.2221-2230.1985;
RA Lok S., Breitman M.L., Chepelinsky A.B., Piatigorsky J., Gold R.J.M.,
RA Tsui L.-C.;
RT "Lens-specific promoter activity of a mouse gamma-crystallin gene.";
RL Mol. Cell. Biol. 5:2221-2230(1985).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 4-121.
RC STRAIN=102 X C3H;
RX PubMed=9782080; DOI=10.1006/geno.1998.5417;
RA Klopp N., Favor J., Loester J., Lutz R.B., Neuhaeuser-Klaus A.,
RA Prescott A., Pretsch W., Quinlan R.A., Sandilands A., Vrensen G.F.J.M.,
RA Graw J.;
RT "Three murine cataract mutants (Cat2) are defective in different gamma-
RT crystallin genes.";
RL Genomics 52:152-158(1998).
CC -!- FUNCTION: Crystallins are the dominant structural components of the
CC vertebrate eye lens.
CC -!- DEVELOPMENTAL STAGE: In the embryo, expressed by day 12 of gestation.
CC Maximum levels are found at day 30-40 followed by a rapid decline.
CC -!- DOMAIN: Has a two-domain beta-structure, folded into four very similar
CC Greek key motifs.
CC -!- MISCELLANEOUS: There are six different gamma crystallins identified in
CC mouse lens.
CC -!- SIMILARITY: Belongs to the beta/gamma-crystallin family. {ECO:0000305}.
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DR EMBL; AK013953; BAB29080.1; -; mRNA.
DR EMBL; BC056453; AAH56453.1; -; mRNA.
DR EMBL; M64543; AAA53525.1; -; mRNA.
DR EMBL; M11039; AAA03227.1; -; Unassigned_DNA.
DR EMBL; AJ224343; CAA11909.1; -; Genomic_DNA.
DR CCDS; CCDS15018.1; -.
DR PIR; I49618; I49618.
DR RefSeq; NP_081286.1; NM_027010.2.
DR AlphaFoldDB; Q9CXV3; -.
DR SMR; Q9CXV3; -.
DR STRING; 10090.ENSMUSP00000027082; -.
DR PaxDb; Q9CXV3; -.
DR PRIDE; Q9CXV3; -.
DR ProteomicsDB; 284013; -.
DR DNASU; 12969; -.
DR Ensembl; ENSMUST00000027082; ENSMUSP00000027082; ENSMUSG00000025945.
DR GeneID; 12969; -.
DR KEGG; mmu:12969; -.
DR UCSC; uc007bhv.1; mouse.
DR CTD; 12969; -.
DR MGI; MGI:88526; Crygf.
DR VEuPathDB; HostDB:ENSMUSG00000025945; -.
DR eggNOG; ENOG502RXJY; Eukaryota.
DR GeneTree; ENSGT00940000163272; -.
DR HOGENOM; CLU_081883_1_1_1; -.
DR InParanoid; Q9CXV3; -.
DR OMA; YERTHFM; -.
DR OrthoDB; 1220704at2759; -.
DR PhylomeDB; Q9CXV3; -.
DR BioGRID-ORCS; 12969; 2 hits in 50 CRISPR screens.
DR PRO; PR:Q9CXV3; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q9CXV3; protein.
DR Bgee; ENSMUSG00000025945; Expressed in lens of camera-type eye and 12 other tissues.
DR ExpressionAtlas; Q9CXV3; baseline and differential.
DR Genevisible; Q9CXV3; MM.
DR GO; GO:0005212; F:structural constituent of eye lens; IBA:GO_Central.
DR GO; GO:0034614; P:cellular response to reactive oxygen species; ISO:MGI.
DR GO; GO:0001654; P:eye development; IMP:MGI.
DR GO; GO:0002088; P:lens development in camera-type eye; IBA:GO_Central.
DR GO; GO:0007601; P:visual perception; ISO:MGI.
DR InterPro; IPR001064; Beta/gamma_crystallin.
DR InterPro; IPR011024; G_crystallin-like.
DR Pfam; PF00030; Crystall; 2.
DR PRINTS; PR01367; BGCRYSTALLIN.
DR SMART; SM00247; XTALbg; 2.
DR SUPFAM; SSF49695; SSF49695; 1.
DR PROSITE; PS50915; CRYSTALLIN_BETA_GAMMA; 4.
PE 2: Evidence at transcript level;
KW Eye lens protein; Reference proteome; Repeat.
FT CHAIN 1..174
FT /note="Gamma-crystallin F"
FT /id="PRO_0000057600"
FT DOMAIN 2..40
FT /note="Beta/gamma crystallin 'Greek key' 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT DOMAIN 41..83
FT /note="Beta/gamma crystallin 'Greek key' 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT DOMAIN 88..128
FT /note="Beta/gamma crystallin 'Greek key' 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT DOMAIN 129..171
FT /note="Beta/gamma crystallin 'Greek key' 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT REGION 84..87
FT /note="Connecting peptide"
FT CONFLICT 14
FT /note="G -> A (in Ref. 3; AAA53525)"
FT /evidence="ECO:0000305"
FT CONFLICT 68..69
FT /note="QW -> YQ (in Ref. 4; AAA03227)"
FT /evidence="ECO:0000305"
FT CONFLICT 80
FT /note="H -> R (in Ref. 3; AAA53525 and 5; CAA11909)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 174 AA; 21249 MW; 3483661E528C9E99 CRC64;
MGKITFYEDR SFQGRHYECS TDHSNLQPYF SRCNSVRVDS GCWMLYEQPN FAGCQYFLRR
GDYPDYQQWM GFSDSVRSCH LIPHSTSHRI RIYEREDYRG QMVEITDDCS HLQDRFHFSD
FHSFHVMEGY WVLYEMPNYR GRQYLLRPGE YRRYHDWGAM NARVGSLRRI MDFY
