CRGN_MOUSE
ID CRGN_MOUSE Reviewed; 183 AA.
AC Q8VHL5; Q0IJ73; Q0VAU5;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 2.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Gamma-crystallin N {ECO:0000305};
DE AltName: Full=Gamma-N-crystallin {ECO:0000305};
GN Name=Crygn {ECO:0000312|MGI:MGI:2449167};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J; TISSUE=Eye;
RX PubMed=15853812; DOI=10.1111/j.1742-4658.2005.04655.x;
RA Wistow G., Wyatt K., David L., Gao C., Bateman O., Bernstein S.,
RA Tomarev S., Segovia L., Slingsby C., Vihtelic T.;
RT "Gamma-N-crystallin and the evolution of the betagamma-crystallin
RT superfamily in vertebrates.";
RL FEBS J. 272:2276-2291(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=27517863; DOI=10.1371/journal.pone.0161140;
RA Hartwich H., Rosengauer E., Ruettiger L., Wilms V., Waterholter S.K.,
RA Nothwang H.G.;
RT "Functional Role of gamma-Crystallin N in the Auditory Hindbrain.";
RL PLoS ONE 11:E0161140-E0161140(2016).
CC -!- FUNCTION: Crystallins are the dominant structural components of the
CC vertebrate eye lens (Probable). Also plays an important role for
CC integrity and function of auditory nuclei (PubMed:27517863).
CC {ECO:0000269|PubMed:27517863, ECO:0000305}.
CC -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:15853812}.
CC -!- TISSUE SPECIFICITY: Primordially eye-specific. Present in lens nucleus.
CC In the retina, expression in observed in the outer plexiform layer
CC (containing photoreceptors axons and synapses) and photoreceptor outer
CC segments (at protein level) (PubMed:15853812). Also detected in the
CC auditory hindbrain where it is highly expressed in the medial nucleus
CC of the trapezoid body, but also present in other nuclei of the superior
CC olivary complex (PubMed:27517863). {ECO:0000269|PubMed:15853812,
CC ECO:0000269|PubMed:27517863}.
CC -!- DOMAIN: Has a two-domain beta-structure, folded into four very similar
CC Greek key motifs. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the beta/gamma-crystallin family. {ECO:0000305}.
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DR EMBL; AF445456; AAL40969.1; -; mRNA.
DR EMBL; BC120913; AAI20914.1; -; mRNA.
DR EMBL; BC120914; AAI20915.1; -; mRNA.
DR CCDS; CCDS19129.1; -.
DR RefSeq; NP_694716.2; NM_153076.2.
DR AlphaFoldDB; Q8VHL5; -.
DR SMR; Q8VHL5; -.
DR STRING; 10090.ENSMUSP00000035860; -.
DR PhosphoSitePlus; Q8VHL5; -.
DR MaxQB; Q8VHL5; -.
DR PaxDb; Q8VHL5; -.
DR PRIDE; Q8VHL5; -.
DR ProteomicsDB; 277894; -.
DR Antibodypedia; 64394; 53 antibodies from 16 providers.
DR DNASU; 214301; -.
DR Ensembl; ENSMUST00000047119; ENSMUSP00000035860; ENSMUSG00000038135.
DR GeneID; 214301; -.
DR KEGG; mmu:214301; -.
DR UCSC; uc008wsi.2; mouse.
DR CTD; 155051; -.
DR MGI; MGI:2449167; Crygn.
DR VEuPathDB; HostDB:ENSMUSG00000038135; -.
DR eggNOG; ENOG502QV8X; Eukaryota.
DR GeneTree; ENSGT00940000159301; -.
DR HOGENOM; CLU_081883_1_0_1; -.
DR InParanoid; Q8VHL5; -.
DR OMA; WQAHSAN; -.
DR OrthoDB; 1445128at2759; -.
DR PhylomeDB; Q8VHL5; -.
DR BioGRID-ORCS; 214301; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Crygn; mouse.
DR PRO; PR:Q8VHL5; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q8VHL5; protein.
DR Bgee; ENSMUSG00000038135; Expressed in lens of camera-type eye and 54 other tissues.
DR Genevisible; Q8VHL5; MM.
DR GO; GO:0005212; F:structural constituent of eye lens; IBA:GO_Central.
DR GO; GO:0002088; P:lens development in camera-type eye; IBA:GO_Central.
DR GO; GO:0007601; P:visual perception; IBA:GO_Central.
DR InterPro; IPR001064; Beta/gamma_crystallin.
DR InterPro; IPR011024; G_crystallin-like.
DR Pfam; PF00030; Crystall; 2.
DR PRINTS; PR01367; BGCRYSTALLIN.
DR SMART; SM00247; XTALbg; 2.
DR SUPFAM; SSF49695; SSF49695; 1.
DR PROSITE; PS50915; CRYSTALLIN_BETA_GAMMA; 4.
PE 1: Evidence at protein level;
KW Eye lens protein; Reference proteome; Repeat.
FT CHAIN 1..183
FT /note="Gamma-crystallin N"
FT /id="PRO_0000311282"
FT DOMAIN 6..46
FT /note="Beta/gamma crystallin 'Greek key' 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT DOMAIN 47..89
FT /note="Beta/gamma crystallin 'Greek key' 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT DOMAIN 95..136
FT /note="Beta/gamma crystallin 'Greek key' 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT DOMAIN 138..180
FT /note="Beta/gamma crystallin 'Greek key' 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00028"
FT CONFLICT 174
FT /note="Q -> H (in Ref. 1; AAL40969)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 183 AA; 21392 MW; 0FBFE0780C9BF501 CRC64;
MAQRSGKITL YEGKHFTGRK LEVFGDCDNF QDQGFMNRVN SIRVESGAWV CFDHPDFRGQ
QFILEHGDYP EFFRWNGHND HMGSCRPVGM HGEHFRIDIF EGCNFTGQCL EFVEDCPFLQ
SRGWAKSCVN AIKVYGDGAW VLYEEPNYRG RMYVLERGDY RCFSDWGAHS ARVQSLRRVL
NFF
