CRIP2_HUMAN
ID CRIP2_HUMAN Reviewed; 208 AA.
AC P52943; A1A4U1; B7Z6C0; E9PD13;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 175.
DE RecName: Full=Cysteine-rich protein 2;
DE Short=CRP-2;
DE AltName: Full=Protein ESP1;
GN Name=CRIP2; Synonyms=CRP2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=8824798; DOI=10.1006/geno.1996.0028;
RA Karim M.A., Ohta K., Egashira M., Jinno Y., Niikawa N., Matsuda I.,
RA Indo Y.;
RT "Human ESP1/CRP2, a member of the LIM domain protein family:
RT characterization of the cDNA and assignment of the gene locus to chromosome
RT 14q32.3.";
RL Genomics 31:167-176(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Heart;
RX PubMed=8843343; DOI=10.1080/15216549600201831;
RA Tsui S.K.W., Chan P.P.K., Cheuk C.W., Liew C.C., Waye M.M.Y., Fung K.P.,
RA Lee C.Y.;
RT "A novel cDNA encoding for a LIM domain protein located at human chromosome
RT 14q32 as a candidate for leukemic translocation.";
RL Biochem. Mol. Biol. Int. 39:747-754(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor vector.";
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Lung, and Pericardium;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12508121; DOI=10.1038/nature01348;
RA Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C.,
RA Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A.,
RA Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H.,
RA Du H., Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T.,
RA Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B.,
RA Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D.,
RA Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R.,
RA Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S.,
RA Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C.,
RA Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S.,
RA Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C.,
RA Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P.,
RA Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M.,
RA Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V.,
RA Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J.,
RA Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F.,
RA Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F.,
RA Waterston R., Hood L., Weissenbach J.;
RT "The DNA sequence and analysis of human chromosome 14.";
RL Nature 421:601-607(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [9]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-138, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19608861; DOI=10.1126/science.1175371;
RA Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C.,
RA Olsen J.V., Mann M.;
RT "Lysine acetylation targets protein complexes and co-regulates major
RT cellular functions.";
RL Science 325:834-840(2009).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [12]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [13]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
RN [14]
RP STRUCTURE BY NMR OF 1-63.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of the LIM domain of human cysteine-rich protein 2.";
RL Submitted (JAN-2006) to the PDB data bank.
CC -!- SUBUNIT: Interacts with TGFB1I1. {ECO:0000250}.
CC -!- INTERACTION:
CC P52943; Q04206: RELA; NbExp=2; IntAct=EBI-947590, EBI-73886;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P52943-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P52943-2; Sequence=VSP_047074;
CC -!- TISSUE SPECIFICITY: Widespread tissue expression; highest levels in the
CC heart.
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DR EMBL; D42123; BAA07703.1; -; mRNA.
DR EMBL; U36190; AAB03194.1; -; mRNA.
DR EMBL; BT019911; AAV38714.1; -; mRNA.
DR EMBL; AK300092; BAH13206.1; -; mRNA.
DR EMBL; AK315757; BAG38110.1; -; mRNA.
DR EMBL; AL928654; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC000434; AAH00434.1; -; mRNA.
DR EMBL; BC001931; AAH01931.1; -; mRNA.
DR EMBL; BC034151; AAH34151.1; -; mRNA.
DR EMBL; BC128101; AAI28102.1; -; mRNA.
DR CCDS; CCDS10003.1; -. [P52943-1]
DR CCDS; CCDS59246.1; -. [P52943-2]
DR PIR; G02090; G02090.
DR RefSeq; NP_001257766.1; NM_001270837.1. [P52943-2]
DR RefSeq; NP_001257770.1; NM_001270841.1.
DR RefSeq; NP_001303.1; NM_001312.3. [P52943-1]
DR PDB; 2CU8; NMR; -; A=1-63.
DR PDBsum; 2CU8; -.
DR AlphaFoldDB; P52943; -.
DR SMR; P52943; -.
DR BioGRID; 107787; 30.
DR DIP; DIP-49905N; -.
DR IntAct; P52943; 20.
DR MINT; P52943; -.
DR STRING; 9606.ENSP00000426119; -.
DR iPTMnet; P52943; -.
DR PhosphoSitePlus; P52943; -.
DR BioMuta; CRIP2; -.
DR DMDM; 1706133; -.
DR EPD; P52943; -.
DR jPOST; P52943; -.
DR MassIVE; P52943; -.
DR MaxQB; P52943; -.
DR PaxDb; P52943; -.
DR PeptideAtlas; P52943; -.
DR PRIDE; P52943; -.
DR ProteomicsDB; 56553; -. [P52943-1]
DR ProteomicsDB; 6770; -.
DR Antibodypedia; 15064; 223 antibodies from 28 providers.
DR DNASU; 1397; -.
DR Ensembl; ENST00000329146.9; ENSP00000328521.5; ENSG00000182809.11. [P52943-1]
DR Ensembl; ENST00000483017.7; ENSP00000426119.2; ENSG00000182809.11. [P52943-2]
DR GeneID; 1397; -.
DR KEGG; hsa:1397; -.
DR MANE-Select; ENST00000329146.9; ENSP00000328521.5; NM_001312.4; NP_001303.1.
DR UCSC; uc001yrd.3; human. [P52943-1]
DR CTD; 1397; -.
DR DisGeNET; 1397; -.
DR GeneCards; CRIP2; -.
DR HGNC; HGNC:2361; CRIP2.
DR HPA; ENSG00000182809; Tissue enriched (heart).
DR MIM; 601183; gene.
DR neXtProt; NX_P52943; -.
DR OpenTargets; ENSG00000182809; -.
DR PharmGKB; PA26879; -.
DR VEuPathDB; HostDB:ENSG00000182809; -.
DR eggNOG; KOG1700; Eukaryota.
DR GeneTree; ENSGT00940000158683; -.
DR HOGENOM; CLU_054591_2_0_1; -.
DR InParanoid; P52943; -.
DR OMA; YEKPCAE; -.
DR OrthoDB; 1214165at2759; -.
DR PhylomeDB; P52943; -.
DR TreeFam; TF313758; -.
DR PathwayCommons; P52943; -.
DR SignaLink; P52943; -.
DR SIGNOR; P52943; -.
DR BioGRID-ORCS; 1397; 12 hits in 1072 CRISPR screens.
DR ChiTaRS; CRIP2; human.
DR EvolutionaryTrace; P52943; -.
DR GeneWiki; CRIP2; -.
DR GenomeRNAi; 1397; -.
DR Pharos; P52943; Tbio.
DR PRO; PR:P52943; -.
DR Proteomes; UP000005640; Chromosome 14.
DR RNAct; P52943; protein.
DR Bgee; ENSG00000182809; Expressed in apex of heart and 181 other tissues.
DR ExpressionAtlas; P52943; baseline and differential.
DR Genevisible; P52943; HS.
DR GO; GO:0005938; C:cell cortex; IEA:Ensembl.
DR GO; GO:0008270; F:zinc ion binding; TAS:ProtInc.
DR GO; GO:0030097; P:hemopoiesis; IEA:Ensembl.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IEA:Ensembl.
DR InterPro; IPR001781; Znf_LIM.
DR Pfam; PF00412; LIM; 2.
DR SMART; SM00132; LIM; 2.
DR PROSITE; PS00478; LIM_DOMAIN_1; 2.
DR PROSITE; PS50023; LIM_DOMAIN_2; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Alternative splicing; LIM domain; Metal-binding;
KW Phosphoprotein; Reference proteome; Repeat; Zinc.
FT CHAIN 1..208
FT /note="Cysteine-rich protein 2"
FT /id="PRO_0000075710"
FT DOMAIN 5..57
FT /note="LIM zinc-binding 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 126..178
FT /note="LIM zinc-binding 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT REGION 98..119
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 23
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q9DCT8"
FT MOD_RES 104
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT MOD_RES 138
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:19608861"
FT MOD_RES 144
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q9DCT8"
FT VAR_SEQ 1..14
FT /note="MASKCPKCDKTVYF -> MPPHHLLPWLAQVPSAEGELVRLVSRAGGRGACF
FT WPAVTMEMAVAAGCVCKGGGCCHREPSQDHHESQEHRGPLVGSQTCLVHQAEGT (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_047074"
FT TURN 6..8
FT /evidence="ECO:0007829|PDB:2CU8"
FT TURN 14..16
FT /evidence="ECO:0007829|PDB:2CU8"
FT STRAND 17..20
FT /evidence="ECO:0007829|PDB:2CU8"
FT STRAND 23..26
FT /evidence="ECO:0007829|PDB:2CU8"
FT TURN 27..29
FT /evidence="ECO:0007829|PDB:2CU8"
FT STRAND 33..35
FT /evidence="ECO:0007829|PDB:2CU8"
FT STRAND 45..47
FT /evidence="ECO:0007829|PDB:2CU8"
FT STRAND 50..52
FT /evidence="ECO:0007829|PDB:2CU8"
FT TURN 54..56
FT /evidence="ECO:0007829|PDB:2CU8"
FT HELIX 57..61
FT /evidence="ECO:0007829|PDB:2CU8"
SQ SEQUENCE 208 AA; 22493 MW; D32B99F98D51D3B0 CRC64;
MASKCPKCDK TVYFAEKVSS LGKDWHKFCL KCERCSKTLT PGGHAEHDGK PFCHKPCYAT
LFGPKGVNIG GAGSYIYEKP LAEGPQVTGP IEVPAARAEE RKASGPPKGP SRASSVTTFT
GEPNTCPRCS KKVYFAEKVT SLGKDWHRPC LRCERCGKTL TPGGHAEHDG QPYCHKPCYG
ILFGPKGVNT GAVGSYIYDR DPEGKVQP
