CRIS1_HUMAN
ID CRIS1_HUMAN Reviewed; 249 AA.
AC P54107; B5BU98; O00698; Q13248; Q14082; Q96SF6;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 171.
DE RecName: Full=Cysteine-rich secretory protein 1;
DE Short=CRISP-1;
DE AltName: Full=AEG-like protein;
DE AltName: Full=ARP;
DE AltName: Full=Acidic epididymal glycoprotein homolog;
DE Flags: Precursor;
GN Name=CRISP1; Synonyms=AEGL1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT), AND PROTEIN SEQUENCE
RP OF 22-41.
RX PubMed=8665901; DOI=10.1111/j.1432-1033.1996.t01-1-00827.x;
RA Kraetzschmar J., Haendler B., Eberspaecher U., Roostermann D., Donner P.,
RA Schleuning W.-D.;
RT "The human cysteine-rich secretory protein (CRISP) family. Primary
RT structure and tissue distribution of CRISP-1, CRISP-2 and CRISP-3.";
RL Eur. J. Biochem. 236:827-836(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
RX PubMed=8543280;
RA Hayashi M.;
RT "Analysis of the human acidic epididymal glycoprotein-like molecule:
RT isolation of cDNA and tissue localization.";
RL Hokkaido Igaku Zasshi 70:743-753(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG), AND CHARACTERIZATION.
RX PubMed=8838800; DOI=10.1006/geno.1996.0131;
RA Hayashi M., Fujimoto S., Takano H., Ushiki T., Abe K., Ishikura H.,
RA Yoshida M.C., Kirchhoff C., Ishibashi T., Kasahara M.;
RT "Characterization of a human glycoprotein with a potential role in sperm-
RT egg fusion: cDNA cloning, immunohistochemical localization, and chromosomal
RT assignment of the gene (AEGL1).";
RL Genomics 32:367-374(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
RX PubMed=19054851; DOI=10.1038/nmeth.1273;
RA Goshima N., Kawamura Y., Fukumoto A., Miura A., Honma R., Satoh R.,
RA Wakamatsu A., Yamamoto J., Kimura K., Nishikawa T., Andoh T., Iida Y.,
RA Ishikawa K., Ito E., Kagawa N., Kaminaga C., Kanehori K., Kawakami B.,
RA Kenmochi K., Kimura R., Kobayashi M., Kuroita T., Kuwayama H., Maruyama Y.,
RA Matsuo K., Minami K., Mitsubori M., Mori M., Morishita R., Murase A.,
RA Nishikawa A., Nishikawa S., Okamoto T., Sakagami N., Sakamoto Y.,
RA Sasaki Y., Seki T., Sono S., Sugiyama A., Sumiya T., Takayama T.,
RA Takayama Y., Takeda H., Togashi T., Yahata K., Yamada H., Yanagisawa Y.,
RA Endo Y., Imamoto F., Kisu Y., Tanaka S., Isogai T., Imai J., Watanabe S.,
RA Nomura N.;
RT "Human protein factory for converting the transcriptome into an in vitro-
RT expressed proteome.";
RL Nat. Methods 5:1011-1017(2008).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
CC -!- FUNCTION: May have a role in sperm-egg fusion and maturation.
CC -!- SUBCELLULAR LOCATION: Note=Located in the lumen and epithelium of
CC distal ductus efferentes and epididymal ducts, and on the postacrosomal
CC region of the sperm head.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Long;
CC IsoId=P54107-1; Sequence=Displayed;
CC Name=Short; Synonyms=CRISP-1 delta;
CC IsoId=P54107-2; Sequence=VSP_006027, VSP_006028;
CC -!- TISSUE SPECIFICITY: Caput, corpus, and cauda regions of the epididymis,
CC the ductus deferens, sperm and seminal plasma.
CC -!- SIMILARITY: Belongs to the CRISP family. {ECO:0000305}.
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DR EMBL; X95237; CAA64524.1; -; mRNA.
DR EMBL; X95238; CAA64525.1; -; mRNA.
DR EMBL; S80310; AAB35899.1; -; mRNA.
DR EMBL; D38451; BAA07483.1; -; mRNA.
DR EMBL; AB451334; BAG70148.1; -; mRNA.
DR EMBL; AB451479; BAG70293.1; -; mRNA.
DR EMBL; AL359458; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS4931.1; -. [P54107-1]
DR CCDS; CCDS4932.1; -. [P54107-2]
DR PIR; S68681; S68681.
DR PIR; S68684; S68684.
DR RefSeq; NP_001122.2; NM_001131.2. [P54107-1]
DR RefSeq; NP_001192149.1; NM_001205220.1. [P54107-1]
DR RefSeq; NP_733758.1; NM_170609.1. [P54107-2]
DR AlphaFoldDB; P54107; -.
DR SMR; P54107; -.
DR BioGRID; 106676; 6.
DR STRING; 9606.ENSP00000338276; -.
DR GlyGen; P54107; 1 site.
DR PhosphoSitePlus; P54107; -.
DR BioMuta; CRISP1; -.
DR DMDM; 1706134; -.
DR jPOST; P54107; -.
DR MassIVE; P54107; -.
DR PaxDb; P54107; -.
DR PeptideAtlas; P54107; -.
DR PRIDE; P54107; -.
DR ProteomicsDB; 56644; -. [P54107-1]
DR ProteomicsDB; 56645; -. [P54107-2]
DR Antibodypedia; 30849; 132 antibodies from 20 providers.
DR DNASU; 167; -.
DR Ensembl; ENST00000329411.9; ENSP00000331317.5; ENSG00000124812.15. [P54107-2]
DR Ensembl; ENST00000335847.9; ENSP00000338276.4; ENSG00000124812.15. [P54107-1]
DR Ensembl; ENST00000505118.1; ENSP00000427589.1; ENSG00000124812.15. [P54107-1]
DR Ensembl; ENST00000507853.5; ENSP00000425020.1; ENSG00000124812.15. [P54107-2]
DR GeneID; 167; -.
DR KEGG; hsa:167; -.
DR MANE-Select; ENST00000335847.9; ENSP00000338276.4; NM_001131.3; NP_001122.2.
DR UCSC; uc003ozw.3; human. [P54107-1]
DR CTD; 167; -.
DR DisGeNET; 167; -.
DR GeneCards; CRISP1; -.
DR HGNC; HGNC:304; CRISP1.
DR HPA; ENSG00000124812; Tissue enriched (epididymis).
DR MIM; 601193; gene.
DR neXtProt; NX_P54107; -.
DR OpenTargets; ENSG00000124812; -.
DR PharmGKB; PA24605; -.
DR VEuPathDB; HostDB:ENSG00000124812; -.
DR eggNOG; KOG3017; Eukaryota.
DR GeneTree; ENSGT00940000162362; -.
DR HOGENOM; CLU_035730_2_1_1; -.
DR InParanoid; P54107; -.
DR OMA; YDEYTDC; -.
DR PhylomeDB; P54107; -.
DR TreeFam; TF316148; -.
DR PathwayCommons; P54107; -.
DR BioGRID-ORCS; 167; 18 hits in 1060 CRISPR screens.
DR GeneWiki; CRISP1; -.
DR GenomeRNAi; 167; -.
DR Pharos; P54107; Tbio.
DR PRO; PR:P54107; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; P54107; protein.
DR Bgee; ENSG00000124812; Expressed in corpus epididymis and 35 other tissues.
DR ExpressionAtlas; P54107; baseline and differential.
DR Genevisible; P54107; HS.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; HDA:UniProtKB.
DR GO; GO:0005246; F:calcium channel regulator activity; IEA:Ensembl.
DR GO; GO:0007339; P:binding of sperm to zona pellucida; IBA:GO_Central.
DR GO; GO:0007342; P:fusion of sperm to egg plasma membrane involved in single fertilization; TAS:ProtInc.
DR GO; GO:0060046; P:regulation of acrosome reaction; IEA:Ensembl.
DR CDD; cd05383; CAP_CRISP; 1.
DR Gene3D; 1.10.10.740; -; 1.
DR Gene3D; 3.40.33.10; -; 1.
DR InterPro; IPR018244; Allrgn_V5/Tpx1_CS.
DR InterPro; IPR014044; CAP_domain.
DR InterPro; IPR035940; CAP_sf.
DR InterPro; IPR042076; Crisp-like_dom.
DR InterPro; IPR001283; CRISP-related.
DR InterPro; IPR013871; Cysteine_rich_secretory.
DR InterPro; IPR034117; SCP_CRISP.
DR InterPro; IPR003582; ShKT_dom.
DR InterPro; IPR002413; V5_allergen-like.
DR PANTHER; PTHR10334; PTHR10334; 1.
DR Pfam; PF00188; CAP; 1.
DR Pfam; PF08562; Crisp; 1.
DR PRINTS; PR00838; V5ALLERGEN.
DR PRINTS; PR00837; V5TPXLIKE.
DR SMART; SM00198; SCP; 1.
DR SUPFAM; SSF55797; SSF55797; 1.
DR PROSITE; PS01009; CRISP_1; 1.
DR PROSITE; PS01010; CRISP_2; 1.
DR PROSITE; PS51670; SHKT; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Direct protein sequencing; Disulfide bond;
KW Glycoprotein; Reference proteome; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000269|PubMed:8665901"
FT CHAIN 22..249
FT /note="Cysteine-rich secretory protein 1"
FT /id="PRO_0000006260"
FT DOMAIN 45..175
FT /note="SCP"
FT DOMAIN 211..244
FT /note="ShKT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT CARBOHYD 230
FT /note="N-linked (GlcNAc...) asparagine"
FT DISULFID 195..202
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT DISULFID 198..207
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT DISULFID 211..244
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT DISULFID 220..238
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT DISULFID 229..242
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT VAR_SEQ 178
FT /note="E -> D (in isoform Short)"
FT /evidence="ECO:0000303|PubMed:19054851,
FT ECO:0000303|PubMed:8665901"
FT /id="VSP_006027"
FT VAR_SEQ 179..249
FT /note="Missing (in isoform Short)"
FT /evidence="ECO:0000303|PubMed:19054851,
FT ECO:0000303|PubMed:8665901"
FT /id="VSP_006028"
FT CONFLICT 22
FT /note="K -> E (in Ref. 2; AAB35899 and 3; BAA07483)"
FT /evidence="ECO:0000305"
FT CONFLICT 97
FT /note="T -> A (in Ref. 2; AAB35899 and 3; BAA07483)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 249 AA; 28481 MW; 85BED3DDEAD62A9C CRC64;
MEIKHLLFLV AAACLLPMLS MKKKSARDQF NKLVTDLPNV QEEIVNIHNA LRRRVVPPAS
NMLKMSWSEE AAQNARIFSK YCDMTESNPL ERRLPNTFCG ENMHMTSYPV SWSSVIGVWY
SESTSFKHGE WTTTDDDITT DHYTQIVWAT SYLIGCAIAS CRQQGSPRYL YVCHYCHEGN
DPETKNEPYK TGVPCEACPS NCEDKLCTNP CIYYDEYFDC DIQVHYLGCN HSTTILFCKA
TCLCDTEIK
