ID   CRIS2_CAVPO             Reviewed;         244 AA.
AC   Q60477;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Cysteine-rich secretory protein 2;
DE            Short=CRISP-2;
DE   AltName: Full=25 kDa acrosomal autoantigen;
DE   AltName: Full=AA1;
DE   AltName: Full=Autoantigen 1;
DE   AltName: Full=Testis-specific protein TPX-1;
DE   Flags: Precursor;
GN   Name=CRISP2; Synonyms=TPX1;
OS   Cavia porcellus (Guinea pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Hystricomorpha; Caviidae;
OC   Cavia.
OX   NCBI_TaxID=10141;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Hartley; TISSUE=Testis;
RX   PubMed=9115720;
RX   DOI=10.1002/(sici)1098-2795(199606)44:2<221::aid-mrd11>3.0.co;2-5;
RA   Foster J.A., Gerton G.L.;
RT   "Autoantigen 1 of the guinea pig sperm acrosome is the homologue of mouse
RT   Tpx-1 and human TPX1 and is a member of the cysteine-rich secretory protein
RT   (CRISP) family.";
RL   Mol. Reprod. Dev. 44:221-229(1996).
RN   [2]
RP   PROTEIN SEQUENCE OF 22-41.
RX   PubMed=3282555; DOI=10.1095/biolreprod38.2.423;
RA   Hardy D.M., Huang T.T.F. Jr., Driscoll W.J., Tung K.S.K., Wild G.C.;
RT   "Purification and characterization of the primary acrosomal autoantigen of
RT   guinea pig epididymal spermatozoa.";
RL   Biol. Reprod. 38:423-437(1988).
CC   -!- FUNCTION: May regulate some ion channels' activity and therebye
CC       regulate calcium fluxes during sperm capacitation. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with NSUN4 isoform 3. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Testis.
CC   -!- SIMILARITY: Belongs to the CRISP family. {ECO:0000305}.
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DR   EMBL; U35712; AAC52616.1; -; mRNA.
DR   RefSeq; NP_001166405.1; NM_001172934.1.
DR   AlphaFoldDB; Q60477; -.
DR   SMR; Q60477; -.
DR   STRING; 10141.ENSCPOP00000009992; -.
DR   PRIDE; Q60477; -.
DR   Ensembl; ENSCPOT00000011215; ENSCPOP00000009992; ENSCPOG00000011111.
DR   GeneID; 100135505; -.
DR   KEGG; cpoc:100135505; -.
DR   CTD; 7180; -.
DR   eggNOG; KOG3017; Eukaryota.
DR   GeneTree; ENSGT00940000156439; -.
DR   HOGENOM; CLU_035730_2_1_1; -.
DR   InParanoid; Q60477; -.
DR   OMA; KCKATCR; -.
DR   OrthoDB; 1528782at2759; -.
DR   TreeFam; TF316148; -.
DR   Proteomes; UP000005447; Unassembled WGS sequence.
DR   Bgee; ENSCPOG00000011111; Expressed in testis and 9 other tissues.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   CDD; cd05383; CAP_CRISP; 1.
DR   Gene3D; 1.10.10.740; -; 1.
DR   Gene3D; 3.40.33.10; -; 1.
DR   InterPro; IPR018244; Allrgn_V5/Tpx1_CS.
DR   InterPro; IPR014044; CAP_domain.
DR   InterPro; IPR035940; CAP_sf.
DR   InterPro; IPR042076; Crisp-like_dom.
DR   InterPro; IPR001283; CRISP-related.
DR   InterPro; IPR013871; Cysteine_rich_secretory.
DR   InterPro; IPR034117; SCP_CRISP.
DR   InterPro; IPR003582; ShKT_dom.
DR   PANTHER; PTHR10334; PTHR10334; 1.
DR   Pfam; PF00188; CAP; 1.
DR   Pfam; PF08562; Crisp; 1.
DR   PRINTS; PR00837; V5TPXLIKE.
DR   SMART; SM00198; SCP; 1.
DR   SUPFAM; SSF55797; SSF55797; 1.
DR   PROSITE; PS01009; CRISP_1; 1.
DR   PROSITE; PS01010; CRISP_2; 1.
DR   PROSITE; PS51670; SHKT; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Reference proteome; Secreted;
KW   Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000269|PubMed:3282555"
FT   CHAIN           22..244
FT                   /note="Cysteine-rich secretory protein 2"
FT                   /id="PRO_0000006265"
FT   DOMAIN          43..170
FT                   /note="SCP"
FT   DOMAIN          206..239
FT                   /note="ShKT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        190..197
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        193..202
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        206..239
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        215..233
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        224..237
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
SQ   SEQUENCE   244 AA;  27248 MW;  58D8DE6EECE16A12 CRC64;
     MALLPVVVFL ITMLLPCVLT NGKDPAFTAL ITTQSQVQNE IINKHNQLRK SVTPPASNML
     KMEWSREAAV NAQKWANRCT LVHSNPDDRK TSTKCGENLY MSSDPSSWSD AIQSWFDESQ
     DFTFGVGPKS HNAVVGHYTQ LVWYSSYLVG CGIAYCPNQD SLKYYYVCQY CPAGNNVYTK
     NTPYKQGIPC ASCPGHCENG LCTNSCEYED LLSNCESLKN TAGCEHQLLV EKCKATCRCE
     DKIY