CRIS2_HUMAN
ID CRIS2_HUMAN Reviewed; 243 AA.
AC P16562; A8K8M0; Q53FF2; Q5U8Z9; Q7Z7B2;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 03-AUG-2022, entry version 177.
DE RecName: Full=Cysteine-rich secretory protein 2;
DE Short=CRISP-2;
DE AltName: Full=Cancer/testis antigen 36;
DE Short=CT36;
DE AltName: Full=Testis-specific protein TPX-1;
DE Flags: Precursor;
GN Name=CRISP2; Synonyms=GAPDL5, TPX1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=2613236; DOI=10.1016/0888-7543(89)90019-0;
RA Kasahara M., Gutknecht J., Brew K., Spurr N., Goodfellow P.N.;
RT "Cloning and mapping of a testis-specific gene with sequence similarity to
RT a sperm-coating glycoprotein gene.";
RL Genomics 5:527-534(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=8665901; DOI=10.1111/j.1432-1033.1996.t01-1-00827.x;
RA Kraetzschmar J., Haendler B., Eberspaecher U., Roostermann D., Donner P.,
RA Schleuning W.-D.;
RT "The human cysteine-rich secretory protein (CRISP) family. Primary
RT structure and tissue distribution of CRISP-1, CRISP-2 and CRISP-3.";
RL Eur. J. Biochem. 236:827-836(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), AND ALTERNATIVE SPLICING.
RC TISSUE=Testis;
RA Sha J.H., Zhou Z.M., Li J.M.;
RT "Cloning of an isoform of TPX-1 gene related to spermatogenesis.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RA Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y.,
RA Tanaka A., Yokoyama S.;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [9]
RP STRUCTURE BY NMR OF 204-239.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of RSGI RUH-032, a cysteine-rich domain of CRISP-2 from
RT human.";
RL Submitted (NOV-2005) to the PDB data bank.
CC -!- FUNCTION: May regulate some ion channels' activity and therebye
CC regulate calcium fluxes during sperm capacitation. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with NSUN4 isoform 3. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P16562-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P16562-2; Sequence=VSP_053891;
CC -!- TISSUE SPECIFICITY: Testis and epididymis.
CC -!- SIMILARITY: Belongs to the CRISP family. {ECO:0000305}.
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DR EMBL; M25532; AAA61220.1; -; mRNA.
DR EMBL; X95239; CAA64526.1; -; mRNA.
DR EMBL; AY289796; AAP41200.1; -; mRNA.
DR EMBL; AY292862; AAP44113.1; -; mRNA.
DR EMBL; AY292863; AAP44114.1; -; mRNA.
DR EMBL; AY773479; AAV48552.1; -; mRNA.
DR EMBL; AK292385; BAF85074.1; -; mRNA.
DR EMBL; AK223337; BAD97057.1; -; mRNA.
DR EMBL; AL121950; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471081; EAX04340.1; -; Genomic_DNA.
DR EMBL; CH471081; EAX04345.1; -; Genomic_DNA.
DR EMBL; BC022011; AAH22011.1; -; mRNA.
DR EMBL; BC107707; AAI07708.1; -; mRNA.
DR CCDS; CCDS4928.1; -. [P16562-1]
DR PIR; B33329; B33329.
DR RefSeq; NP_001135879.1; NM_001142407.2. [P16562-1]
DR RefSeq; NP_001135880.1; NM_001142408.2. [P16562-1]
DR RefSeq; NP_001135889.1; NM_001142417.2. [P16562-1]
DR RefSeq; NP_001135907.1; NM_001142435.2. [P16562-1]
DR RefSeq; NP_001248751.1; NM_001261822.1. [P16562-1]
DR RefSeq; NP_003287.1; NM_003296.3. [P16562-1]
DR RefSeq; XP_005249406.1; XM_005249349.1. [P16562-2]
DR RefSeq; XP_005249407.1; XM_005249350.1. [P16562-2]
DR RefSeq; XP_005249408.1; XM_005249351.3. [P16562-2]
DR RefSeq; XP_005249409.1; XM_005249352.3. [P16562-2]
DR RefSeq; XP_005249410.1; XM_005249353.3. [P16562-2]
DR RefSeq; XP_005249413.1; XM_005249356.1. [P16562-2]
DR RefSeq; XP_011513143.1; XM_011514841.1. [P16562-2]
DR RefSeq; XP_011513144.1; XM_011514842.1. [P16562-2]
DR PDB; 2CQ7; NMR; -; A=204-239.
DR PDBsum; 2CQ7; -.
DR AlphaFoldDB; P16562; -.
DR SMR; P16562; -.
DR BioGRID; 113032; 50.
DR IntAct; P16562; 6.
DR STRING; 9606.ENSP00000339155; -.
DR iPTMnet; P16562; -.
DR PhosphoSitePlus; P16562; -.
DR BioMuta; CRISP2; -.
DR DMDM; 136111; -.
DR MassIVE; P16562; -.
DR PaxDb; P16562; -.
DR PeptideAtlas; P16562; -.
DR PRIDE; P16562; -.
DR ProteomicsDB; 53380; -. [P16562-1]
DR ProteomicsDB; 69511; -.
DR Antibodypedia; 30834; 237 antibodies from 37 providers.
DR DNASU; 7180; -.
DR Ensembl; ENST00000339139.5; ENSP00000339155.4; ENSG00000124490.14. [P16562-1]
DR Ensembl; ENST00000616725.4; ENSP00000484609.1; ENSG00000124490.14. [P16562-1]
DR Ensembl; ENST00000618917.4; ENSP00000482890.1; ENSG00000124490.14. [P16562-2]
DR GeneID; 7180; -.
DR KEGG; hsa:7180; -.
DR MANE-Select; ENST00000339139.5; ENSP00000339155.4; NM_003296.4; NP_003287.1.
DR UCSC; uc003ozl.4; human. [P16562-1]
DR CTD; 7180; -.
DR DisGeNET; 7180; -.
DR GeneCards; CRISP2; -.
DR HGNC; HGNC:12024; CRISP2.
DR HPA; ENSG00000124490; Tissue enriched (testis).
DR MIM; 187430; gene.
DR neXtProt; NX_P16562; -.
DR OpenTargets; ENSG00000124490; -.
DR PharmGKB; PA36703; -.
DR VEuPathDB; HostDB:ENSG00000124490; -.
DR eggNOG; KOG3017; Eukaryota.
DR GeneTree; ENSGT00940000156439; -.
DR HOGENOM; CLU_035730_2_1_1; -.
DR InParanoid; P16562; -.
DR OMA; KCKATCR; -.
DR OrthoDB; 1528782at2759; -.
DR PhylomeDB; P16562; -.
DR TreeFam; TF316148; -.
DR PathwayCommons; P16562; -.
DR SignaLink; P16562; -.
DR BioGRID-ORCS; 7180; 10 hits in 1060 CRISPR screens.
DR EvolutionaryTrace; P16562; -.
DR GeneWiki; CRISP2; -.
DR GenomeRNAi; 7180; -.
DR Pharos; P16562; Tbio.
DR PRO; PR:P16562; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; P16562; protein.
DR Bgee; ENSG00000124490; Expressed in sperm and 116 other tissues.
DR ExpressionAtlas; P16562; baseline and differential.
DR Genevisible; P16562; HS.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR CDD; cd05383; CAP_CRISP; 1.
DR Gene3D; 1.10.10.740; -; 1.
DR Gene3D; 3.40.33.10; -; 1.
DR InterPro; IPR018244; Allrgn_V5/Tpx1_CS.
DR InterPro; IPR014044; CAP_domain.
DR InterPro; IPR035940; CAP_sf.
DR InterPro; IPR042076; Crisp-like_dom.
DR InterPro; IPR001283; CRISP-related.
DR InterPro; IPR013871; Cysteine_rich_secretory.
DR InterPro; IPR034117; SCP_CRISP.
DR InterPro; IPR003582; ShKT_dom.
DR PANTHER; PTHR10334; PTHR10334; 1.
DR Pfam; PF00188; CAP; 1.
DR Pfam; PF08562; Crisp; 1.
DR PRINTS; PR00837; V5TPXLIKE.
DR SMART; SM00198; SCP; 1.
DR SUPFAM; SSF55797; SSF55797; 1.
DR PROSITE; PS01009; CRISP_1; 1.
DR PROSITE; PS01010; CRISP_2; 1.
DR PROSITE; PS51670; SHKT; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Disulfide bond; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..243
FT /note="Cysteine-rich secretory protein 2"
FT /id="PRO_0000006264"
FT DOMAIN 41..169
FT /note="SCP"
FT DOMAIN 205..238
FT /note="ShKT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT DISULFID 189..196
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT DISULFID 192..201
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT DISULFID 205..238
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT DISULFID 214..232
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT DISULFID 223..236
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT VAR_SEQ 172
FT /note="A -> AMKTYLNKREGINVWKCFLRLRHFQLLRGEQLLTFS (in
FT isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_053891"
FT VARIANT 131
FT /note="N -> S (in dbSNP:rs34457011)"
FT /id="VAR_048832"
FT CONFLICT 64
FT /note="S -> C (in Ref. 4; BAF85074)"
FT /evidence="ECO:0000305"
FT CONFLICT 88
FT /note="R -> C (in Ref. 5; BAD97057)"
FT /evidence="ECO:0000305"
FT STRAND 210..213
FT /evidence="ECO:0007829|PDB:2CQ7"
FT HELIX 214..218
FT /evidence="ECO:0007829|PDB:2CQ7"
FT TURN 219..221
FT /evidence="ECO:0007829|PDB:2CQ7"
FT STRAND 225..230
FT /evidence="ECO:0007829|PDB:2CQ7"
FT HELIX 233..236
FT /evidence="ECO:0007829|PDB:2CQ7"
SQ SEQUENCE 243 AA; 27259 MW; C5FE698C449CFAD9 CRC64;
MALLPVLFLV TVLLPSLPAE GKDPAFTALL TTQLQVQREI VNKHNELRKA VSPPASNMLK
MEWSREVTTN AQRWANKCTL QHSDPEDRKT STRCGENLYM SSDPTSWSSA IQSWYDEILD
FVYGVGPKSP NAVVGHYTQL VWYSTYQVGC GIAYCPNQDS LKYYYVCQYC PAGNNMNRKN
TPYQQGTPCA GCPDDCDKGL CTNSCQYQDL LSNCDSLKNT AGCEHELLKE KCKATCLCEN
KIY
