CRIS3_HUMAN
ID CRIS3_HUMAN Reviewed; 245 AA.
AC P54108; A8K9S1; B2R8I8; Q15512; Q3MJ82; Q53FA9; Q5JW83; Q9H108;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 175.
DE RecName: Full=Cysteine-rich secretory protein 3;
DE Short=CRISP-3;
DE AltName: Full=Specific granule protein of 28 kDa;
DE Short=SGP28;
DE Flags: Precursor;
GN Name=CRISP3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RX PubMed=8601434; DOI=10.1016/0014-5793(96)00030-0;
RA Kjeldsen L., Cowland J.B., Johnson A.H., Borregaard N.;
RT "SGP28, a novel matrix glycoprotein in specific granules of human
RT neutrophils with similarity to a human testis-specific gene product and a
RT rodent sperm-coating glycoprotein.";
RL FEBS Lett. 380:246-250(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE
RP [MRNA] OF 1-155 (ISOFORM 3), AND VARIANTS PRO-106 AND SER-134.
RC TISSUE=Trachea;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Gastric mucosa;
RA Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), PARTIAL NUCLEOTIDE
RP SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND VARIANT PRO-106.
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RX PubMed=8665901; DOI=10.1111/j.1432-1033.1996.t01-1-00827.x;
RA Kraetzschmar J., Haendler B., Eberspaecher U., Roostermann D., Donner P.,
RA Schleuning W.-D.;
RT "The human cysteine-rich secretory protein (CRISP) family. Primary
RT structure and tissue distribution of CRISP-1, CRISP-2 and CRISP-3.";
RL Eur. J. Biochem. 236:827-836(1996).
RN [7]
RP INTERACTION WITH A1BG.
RX PubMed=15461460; DOI=10.1021/bi048823e;
RA Udby L., Sorensen O.E., Pass J., Johnsen A.H., Behrendt N., Borregaard N.,
RA Kjeldsen L.;
RT "Cysteine-rich secretory protein 3 is a ligand of alpha1B-glycoprotein in
RT human plasma.";
RL Biochemistry 43:12877-12886(2004).
CC -!- SUBUNIT: Interacts with A1BG. {ECO:0000269|PubMed:15461460}.
CC -!- INTERACTION:
CC P54108-2; Q8TDT2: GPR152; NbExp=3; IntAct=EBI-18210151, EBI-13345167;
CC -!- SUBCELLULAR LOCATION: Secreted. Note=In neutrophils, localized in
CC specific granules.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=P54108-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P54108-2; Sequence=VSP_042758;
CC Name=3;
CC IsoId=P54108-3; Sequence=VSP_042759;
CC -!- TISSUE SPECIFICITY: Salivary gland, pancreas and prostate > epididymis,
CC ovary, thymus and colon.
CC -!- SIMILARITY: Belongs to the CRISP family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH69580.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAH69602.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAG36185.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAA63984.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAA64527.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; X94323; CAA63984.1; ALT_INIT; mRNA.
DR EMBL; AK292786; BAF85475.1; -; mRNA.
DR EMBL; AK313387; BAG36185.1; ALT_INIT; mRNA.
DR EMBL; DB205467; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK223380; BAD97100.1; -; mRNA.
DR EMBL; AL121950; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL121974; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC069580; AAH69580.1; ALT_INIT; mRNA.
DR EMBL; BC069602; AAH69602.1; ALT_INIT; mRNA.
DR EMBL; BC101539; AAI01540.1; -; mRNA.
DR EMBL; X95240; CAA64527.1; ALT_INIT; mRNA.
DR CCDS; CCDS4929.2; -. [P54108-2]
DR CCDS; CCDS55019.1; -. [P54108-3]
DR PIR; S68691; S68691.
DR RefSeq; NP_001177915.1; NM_001190986.2. [P54108-3]
DR RefSeq; NP_006052.2; NM_006061.3. [P54108-2]
DR AlphaFoldDB; P54108; -.
DR SMR; P54108; -.
DR BioGRID; 115605; 19.
DR IntAct; P54108; 1.
DR STRING; 9606.ENSP00000389026; -.
DR GlyConnect; 1163; 2 N-Linked glycans (1 site).
DR GlyGen; P54108; 2 sites, 2 N-linked glycans (1 site), 1 O-linked glycan (1 site).
DR iPTMnet; P54108; -.
DR PhosphoSitePlus; P54108; -.
DR BioMuta; CRISP3; -.
DR DMDM; 1706135; -.
DR jPOST; P54108; -.
DR MassIVE; P54108; -.
DR PaxDb; P54108; -.
DR PeptideAtlas; P54108; -.
DR PRIDE; P54108; -.
DR ProteomicsDB; 56646; -. [P54108-1]
DR ProteomicsDB; 56647; -. [P54108-2]
DR ProteomicsDB; 56648; -. [P54108-3]
DR TopDownProteomics; P54108-1; -. [P54108-1]
DR Antibodypedia; 30841; 207 antibodies from 28 providers.
DR DNASU; 10321; -.
DR Ensembl; ENST00000263045.9; ENSP00000263045.4; ENSG00000096006.12. [P54108-2]
DR Ensembl; ENST00000433368.6; ENSP00000389026.2; ENSG00000096006.12. [P54108-3]
DR GeneID; 10321; -.
DR KEGG; hsa:10321; -.
DR MANE-Select; ENST00000263045.9; ENSP00000263045.4; NM_006061.4; NP_006052.2. [P54108-2]
DR UCSC; uc003ozs.5; human. [P54108-1]
DR CTD; 10321; -.
DR DisGeNET; 10321; -.
DR GeneCards; CRISP3; -.
DR HGNC; HGNC:16904; CRISP3.
DR HPA; ENSG00000096006; Group enriched (fallopian tube, salivary gland).
DR MIM; 618062; gene.
DR neXtProt; NX_P54108; -.
DR OpenTargets; ENSG00000096006; -.
DR PharmGKB; PA134880440; -.
DR VEuPathDB; HostDB:ENSG00000096006; -.
DR eggNOG; KOG3017; Eukaryota.
DR GeneTree; ENSGT00940000162013; -.
DR InParanoid; P54108; -.
DR OMA; YTHPYKS; -.
DR OrthoDB; 1528782at2759; -.
DR PhylomeDB; P54108; -.
DR TreeFam; TF316148; -.
DR PathwayCommons; P54108; -.
DR Reactome; R-HSA-6798695; Neutrophil degranulation.
DR SignaLink; P54108; -.
DR BioGRID-ORCS; 10321; 7 hits in 1059 CRISPR screens.
DR ChiTaRS; CRISP3; human.
DR GeneWiki; CRISP3; -.
DR GenomeRNAi; 10321; -.
DR Pharos; P54108; Tbio.
DR PRO; PR:P54108; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; P54108; protein.
DR Bgee; ENSG00000096006; Expressed in pancreatic ductal cell and 123 other tissues.
DR ExpressionAtlas; P54108; baseline and differential.
DR Genevisible; P54108; HS.
DR GO; GO:0031012; C:extracellular matrix; NAS:UniProtKB.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR GO; GO:0005615; C:extracellular space; HDA:UniProtKB.
DR GO; GO:0042581; C:specific granule; IDA:UniProtKB.
DR GO; GO:0035580; C:specific granule lumen; TAS:Reactome.
DR GO; GO:1904724; C:tertiary granule lumen; TAS:Reactome.
DR GO; GO:0006952; P:defense response; NAS:UniProtKB.
DR GO; GO:0045087; P:innate immune response; NAS:UniProtKB.
DR CDD; cd05383; CAP_CRISP; 1.
DR Gene3D; 1.10.10.740; -; 1.
DR Gene3D; 3.40.33.10; -; 1.
DR InterPro; IPR018244; Allrgn_V5/Tpx1_CS.
DR InterPro; IPR014044; CAP_domain.
DR InterPro; IPR035940; CAP_sf.
DR InterPro; IPR042076; Crisp-like_dom.
DR InterPro; IPR001283; CRISP-related.
DR InterPro; IPR013871; Cysteine_rich_secretory.
DR InterPro; IPR034117; SCP_CRISP.
DR InterPro; IPR003582; ShKT_dom.
DR PANTHER; PTHR10334; PTHR10334; 1.
DR Pfam; PF00188; CAP; 1.
DR Pfam; PF08562; Crisp; 1.
DR PRINTS; PR00837; V5TPXLIKE.
DR SMART; SM00198; SCP; 1.
DR SUPFAM; SSF55797; SSF55797; 1.
DR PROSITE; PS01009; CRISP_1; 1.
DR PROSITE; PS01010; CRISP_2; 1.
DR PROSITE; PS51670; SHKT; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Disulfide bond; Glycoprotein; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..245
FT /note="Cysteine-rich secretory protein 3"
FT /id="PRO_0000006268"
FT DOMAIN 43..171
FT /note="SCP"
FT DOMAIN 207..240
FT /note="ShKT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT CARBOHYD 239
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 191..198
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT DISULFID 194..203
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT DISULFID 207..240
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT DISULFID 216..234
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT DISULFID 225..238
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT VAR_SEQ 1
FT /note="M -> MKQILHPALETTAM (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:8601434"
FT /id="VSP_042758"
FT VAR_SEQ 1
FT /note="M -> MKQILHPALETTDPCSTGFVFPAM (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_042759"
FT VARIANT 106
FT /note="S -> P (in dbSNP:rs495335)"
FT /evidence="ECO:0000269|PubMed:14702039,
FT ECO:0000269|PubMed:15489334"
FT /id="VAR_011718"
FT VARIANT 134
FT /note="A -> S (in dbSNP:rs1864312)"
FT /evidence="ECO:0000269|PubMed:14702039"
FT /id="VAR_011719"
FT CONFLICT 79
FT /note="Q -> R (in Ref. 2; BAF85475)"
FT /evidence="ECO:0000305"
FT CONFLICT 130
FT /note="K -> R (in Ref. 3; BAD97100)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 245 AA; 27630 MW; B4DD79CB7AE9E5F9 CRC64;
MTLFPVLLFL VAGLLPSFPA NEDKDPAFTA LLTTQTQVQR EIVNKHNELR RAVSPPARNM
LKMEWNKEAA ANAQKWANQC NYRHSNPKDR MTSLKCGENL YMSSASSSWS QAIQSWFDEY
NDFDFGVGPK TPNAVVGHYT QVVWYSSYLV GCGNAYCPNQ KVLKYYYVCQ YCPAGNWANR
LYVPYEQGAP CASCPDNCDD GLCTNGCKYE DLYSNCKSLK LTLTCKHQLV RDSCKASCNC
SNSIY
