CRLA_DICDI
ID CRLA_DICDI Reviewed; 369 AA.
AC Q54U75; Q86GL6;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 83.
DE RecName: Full=Cyclic AMP receptor-like protein A;
GN Name=crlA; ORFNames=DDB_G0280983;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DEVELOPMENTAL STAGE, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=14732403; DOI=10.1016/j.ydbio.2003.09.035;
RA Raisley B., Zhang M., Hereld D., Hadwiger J.A.;
RT "A cAMP receptor-like G protein-coupled receptor with roles in growth
RT regulation and development.";
RL Dev. Biol. 265:433-445(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP NOMENCLATURE.
RX PubMed=16735079; DOI=10.1016/j.ejcb.2006.04.003;
RA Prabhu Y., Eichinger L.;
RT "The Dictyostelium repertoire of seven transmembrane domain receptors.";
RL Eur. J. Cell Biol. 85:937-946(2006).
CC -!- FUNCTION: Receptor for cAMP which may play a role in prestalk cell
CC differentiation. May act as a negative regulator of cell growth.
CC {ECO:0000269|PubMed:14732403}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed during vegetative growth and throughout
CC development. {ECO:0000269|PubMed:14732403}.
CC -!- DISRUPTION PHENOTYPE: Cells grow to a higher cell density than wild-
CC type cells. They also produce large aggregates with delayed anterior
CC tip formation indicating a role in the development of the anterior
CC prestalk cell region. {ECO:0000269|PubMed:14732403}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 5 family.
CC {ECO:0000305}.
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DR EMBL; AY219179; AAO62367.1; -; Genomic_DNA.
DR EMBL; AAFI02000040; EAL66788.1; -; Genomic_DNA.
DR RefSeq; XP_640901.1; XM_635809.1.
DR AlphaFoldDB; Q54U75; -.
DR SMR; Q54U75; -.
DR STRING; 44689.DDB0215007; -.
DR PaxDb; Q54U75; -.
DR EnsemblProtists; EAL66788; EAL66788; DDB_G0280983.
DR GeneID; 8622960; -.
DR KEGG; ddi:DDB_G0280983; -.
DR dictyBase; DDB_G0280983; crlA.
DR eggNOG; ENOG502RSRC; Eukaryota.
DR HOGENOM; CLU_751059_0_0_1; -.
DR InParanoid; Q54U75; -.
DR OMA; PRICIVQ; -.
DR PhylomeDB; Q54U75; -.
DR PRO; PR:Q54U75; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0016021; C:integral component of membrane; ISS:dictyBase.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0001646; F:cAMP receptor activity; ISS:dictyBase.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0030308; P:negative regulation of cell growth; IMP:dictyBase.
DR GO; GO:0007165; P:signal transduction; IMP:dictyBase.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IMP:dictyBase.
DR InterPro; IPR022343; GCR1-cAMP_receptor.
DR InterPro; IPR017981; GPCR_2-like.
DR InterPro; IPR022340; GPCR_GCR1_put.
DR PRINTS; PR02001; GCR1CAMPR.
DR PRINTS; PR02000; GCR1PLANT.
DR PROSITE; PS50261; G_PROTEIN_RECEP_F2_4; 1.
PE 2: Evidence at transcript level;
KW G-protein coupled receptor; Membrane; Receptor; Reference proteome;
KW Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..369
FT /note="Cyclic AMP receptor-like protein A"
FT /id="PRO_0000327674"
FT TOPO_DOM 1..4
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 5..22
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 23..72
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..93
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 94..100
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 101..121
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 122..146
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 168..173
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 174..194
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 195..212
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 213..233
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 234..247
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 248..268
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 269..369
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 317
FT /note="F -> L (in Ref. 1; AAO62367)"
FT /evidence="ECO:0000305"
FT CONFLICT 320
FT /note="Y -> N (in Ref. 1; AAO62367)"
FT /evidence="ECO:0000305"
FT CONFLICT 327
FT /note="R -> K (in Ref. 1; AAO62367)"
FT /evidence="ECO:0000305"
FT CONFLICT 334
FT /note="E -> K (in Ref. 1; AAO62367)"
FT /evidence="ECO:0000305"
FT CONFLICT 349
FT /note="S -> R (in Ref. 1; AAO62367)"
FT /evidence="ECO:0000305"
FT CONFLICT 353
FT /note="E -> K (in Ref. 1; AAO62367)"
FT /evidence="ECO:0000305"
FT CONFLICT 364
FT /note="D -> E (in Ref. 1; AAO62367)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 369 AA; 42976 MW; 63286125E9565C24 CRC64;
MIQILLSTFI SFIIIIVSSN DIRSGENDNF NNNKMINNFL TTITTNDTII IKETESPNDY
DFSKEQIESL DKIVYFSSTM GIVGALFIIV SFFLFKAART FATKMIFFLS LSDLFAAIFY
LPYYRDSDIM CNLQGMGLVF FLSSSYLWTM CISISLFMVF FTTIFELNHW FKYFHFICWG
IPLFTAIISL IFHAYGKTGS WCFISDPTSI FRLLYYLPLI VVFFINLVVF IAIRWKISQH
SNSLVSRVNI IVSFYLIAFS LSQLPTIINS IQNFSDPDNP QFSLFAFQLL LQPLQGFLNC
VVYGINEGFI NHYVEFFEKY IFRCRCRKSR ELKEIESDKT SLLVDYENSD DEEGFDGMDK
LIIDDYNRV
