CRLD1_HUMAN
ID CRLD1_HUMAN Reviewed; 500 AA.
AC Q9H336; B2RA60; B7Z929;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Cysteine-rich secretory protein LCCL domain-containing 1;
DE AltName: Full=CocoaCrisp;
DE AltName: Full=Cysteine-rich secretory protein 10;
DE Short=CRISP-10;
DE AltName: Full=LCCL domain-containing cysteine-rich secretory protein 1;
DE AltName: Full=Trypsin inhibitor Hl;
DE Flags: Precursor;
GN Name=CRISPLD1; Synonyms=CRISP10, LCRISP1; ORFNames=UNQ342/PRO541;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Aorta;
RA Zhao Y., Cao H., Jiang Y., Meng X., Zhao X., Liu D., Ding J.;
RT "Cloning and characterization of a trypsin inhibitor-like protein gene in
RT human aorta.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Heart, and Testis;
RA Smith D.M., Collins-Racie L.A., Lavallie E.R., Gamer L., Roberts D.J.,
RA Marigo V.A., Copeland N.G., Jenkins N.A., McCoy J., Tabin C.J.;
RT "A novel cysteine-rich secreted protein (CRISP) family member, CocoaCrisp,
RT provides insight into the process of septation in the developing chicken
RT midbrain.";
RL Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Trachea;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Melanoma;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16421571; DOI=10.1038/nature04406;
RA Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M.,
RA Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L.,
RA Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S.,
RA Asakawa T., Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A.,
RA Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III,
RA Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K.,
RA Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P.,
RA Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H.,
RA Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B.,
RA O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K.,
RA Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L.,
RA Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G.,
RA Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W.,
RA Platzer M., Shimizu N., Lander E.S.;
RT "DNA sequence and analysis of human chromosome 8.";
RL Nature 439:331-335(2006).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Cervix adenocarcinoma;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9H336-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9H336-2; Sequence=VSP_055281, VSP_055282;
CC -!- SIMILARITY: Belongs to the CRISP family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF142573; AAG43287.1; -; mRNA.
DR EMBL; AF329197; AAK16495.1; -; mRNA.
DR EMBL; AY358564; AAQ88927.1; -; mRNA.
DR EMBL; AK304350; BAH14165.1; -; mRNA.
DR EMBL; AK314048; BAG36757.1; -; mRNA.
DR EMBL; AL834301; CAH56361.1; -; mRNA.
DR EMBL; AC100782; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC102801; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471068; EAW87038.1; -; Genomic_DNA.
DR EMBL; BC020514; AAH20514.1; -; mRNA.
DR CCDS; CCDS6219.1; -. [Q9H336-1]
DR CCDS; CCDS69497.1; -. [Q9H336-2]
DR RefSeq; NP_001273706.1; NM_001286777.1. [Q9H336-2]
DR RefSeq; NP_113649.1; NM_031461.5. [Q9H336-1]
DR AlphaFoldDB; Q9H336; -.
DR SMR; Q9H336; -.
DR BioGRID; 123725; 12.
DR STRING; 9606.ENSP00000262207; -.
DR iPTMnet; Q9H336; -.
DR PhosphoSitePlus; Q9H336; -.
DR BioMuta; CRISPLD1; -.
DR DMDM; 74733568; -.
DR EPD; Q9H336; -.
DR jPOST; Q9H336; -.
DR MassIVE; Q9H336; -.
DR PaxDb; Q9H336; -.
DR PeptideAtlas; Q9H336; -.
DR PRIDE; Q9H336; -.
DR ProteomicsDB; 6999; -.
DR ProteomicsDB; 80668; -. [Q9H336-1]
DR Antibodypedia; 12356; 90 antibodies from 18 providers.
DR DNASU; 83690; -.
DR Ensembl; ENST00000262207.9; ENSP00000262207.4; ENSG00000121005.9. [Q9H336-1]
DR Ensembl; ENST00000517786.1; ENSP00000429746.1; ENSG00000121005.9. [Q9H336-2]
DR GeneID; 83690; -.
DR KEGG; hsa:83690; -.
DR MANE-Select; ENST00000262207.9; ENSP00000262207.4; NM_031461.6; NP_113649.1.
DR UCSC; uc003yan.5; human. [Q9H336-1]
DR CTD; 83690; -.
DR DisGeNET; 83690; -.
DR GeneCards; CRISPLD1; -.
DR HGNC; HGNC:18206; CRISPLD1.
DR HPA; ENSG00000121005; Tissue enhanced (breast).
DR neXtProt; NX_Q9H336; -.
DR OpenTargets; ENSG00000121005; -.
DR PharmGKB; PA142672076; -.
DR VEuPathDB; HostDB:ENSG00000121005; -.
DR eggNOG; KOG3017; Eukaryota.
DR GeneTree; ENSGT00940000156473; -.
DR HOGENOM; CLU_042287_0_0_1; -.
DR InParanoid; Q9H336; -.
DR OMA; DGEWWMA; -.
DR OrthoDB; 1528782at2759; -.
DR PhylomeDB; Q9H336; -.
DR TreeFam; TF316148; -.
DR PathwayCommons; Q9H336; -.
DR BioGRID-ORCS; 83690; 9 hits in 1072 CRISPR screens.
DR GenomeRNAi; 83690; -.
DR Pharos; Q9H336; Tbio.
DR PRO; PR:Q9H336; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; Q9H336; protein.
DR Bgee; ENSG00000121005; Expressed in tibia and 154 other tissues.
DR ExpressionAtlas; Q9H336; baseline and differential.
DR Genevisible; Q9H336; HS.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0060325; P:face morphogenesis; IMP:UniProtKB.
DR GO; GO:0061484; P:hematopoietic stem cell homeostasis; IEA:Ensembl.
DR Gene3D; 2.170.130.20; -; 2.
DR Gene3D; 3.40.33.10; -; 1.
DR InterPro; IPR018244; Allrgn_V5/Tpx1_CS.
DR InterPro; IPR014044; CAP_domain.
DR InterPro; IPR035940; CAP_sf.
DR InterPro; IPR001283; CRISP-related.
DR InterPro; IPR004043; LCCL.
DR InterPro; IPR036609; LCCL_sf.
DR PANTHER; PTHR10334; PTHR10334; 1.
DR Pfam; PF00188; CAP; 1.
DR Pfam; PF03815; LCCL; 2.
DR PRINTS; PR00837; V5TPXLIKE.
DR SMART; SM00603; LCCL; 2.
DR SMART; SM00198; SCP; 1.
DR SUPFAM; SSF55797; SSF55797; 1.
DR SUPFAM; SSF69848; SSF69848; 2.
DR PROSITE; PS01010; CRISP_2; 1.
DR PROSITE; PS50820; LCCL; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Disulfide bond; Reference proteome; Repeat; Secreted;
KW Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..500
FT /note="Cysteine-rich secretory protein LCCL domain-
FT containing 1"
FT /id="PRO_0000248146"
FT DOMAIN 66..206
FT /note="SCP"
FT DOMAIN 289..384
FT /note="LCCL 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00123"
FT DOMAIN 390..492
FT /note="LCCL 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00123"
FT REGION 254..281
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 254..279
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 295..313
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00123"
FT DISULFID 317..337
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00123"
FT DISULFID 396..418
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00123"
FT DISULFID 422..445
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00123"
FT VAR_SEQ 1..23
FT /note="MKCTAREWLRVTTVLFMARAIPA -> MNMNATHIVHSGVLALYVHIIHR
FT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_055281"
FT VAR_SEQ 24..209
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_055282"
FT VARIANT 286
FT /note="A -> S (in dbSNP:rs1945)"
FT /id="VAR_027255"
SQ SEQUENCE 500 AA; 56888 MW; 203B1DC4DDA003CB CRC64;
MKCTAREWLR VTTVLFMARA IPAMVVPNAT LLEKLLEKYM DEDGEWWIAK QRGKRAITDN
DMQSILDLHN KLRSQVYPTA SNMEYMTWDV ELERSAESWA ESCLWEHGPA SLLPSIGQNL
GAHWGRYRPP TFHVQSWYDE VKDFSYPYEH ECNPYCPFRC SGPVCTHYTQ VVWATSNRIG
CAINLCHNMN IWGQIWPKAV YLVCNYSPKG NWWGHAPYKH GRPCSACPPS FGGGCRENLC
YKEGSDRYYP PREEETNEIE RQQSQVHDTH VRTRSDDSSR NEVISAQQMS QIVSCEVRLR
DQCKGTTCNR YECPAGCLDS KAKVIGSVHY EMQSSICRAA IHYGIIDNDG GWVDITRQGR
KHYFIKSNRN GIQTIGKYQS ANSFTVSKVT VQAVTCETTV EQLCPFHKPA SHCPRVYCPR
NCMQANPHYA RVIGTRVYSD LSSICRAAVH AGVVRNHGGY VDVMPVDKRK TYIASFQNGI
FSESLQNPPG GKAFRVFAVV
