CRLD2_HUMAN
ID CRLD2_HUMAN Reviewed; 497 AA.
AC Q9H0B8; D3DUM0; Q6P590; Q6UWH0; Q6UXC6; Q96IB1; Q96K61;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Cysteine-rich secretory protein LCCL domain-containing 2;
DE AltName: Full=Cysteine-rich secretory protein 11;
DE Short=CRISP-11;
DE AltName: Full=LCCL domain-containing cysteine-rich secretory protein 2;
DE Flags: Precursor;
GN Name=CRISPLD2; Synonyms=CRISP11, LCRISP2; ORFNames=UNQ2914/PRO1156/PRO9783;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=11230166; DOI=10.1101/gr.gr1547r;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J.,
RA Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W.,
RA Ottenwaelder B., Obermaier B., Tampe J., Heubner D., Wambutt R., Korn B.,
RA Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and analysis of
RT 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4), AND VARIANT
RP SER-322.
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Mammary gland;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT GLY-105.
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 5), AND VARIANTS
RP GLY-105 AND SER-322.
RC TISSUE=Blood, and Melanoma;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP VARIANT GLY-137.
RX PubMed=25691535; DOI=10.1093/hmg/ddv060;
RA Guipponi M., Chentouf A., Webling K.E., Freimann K., Crespel A., Nobile C.,
RA Lemke J.R., Hansen J., Dorn T., Lesca G., Ryvlin P., Hirsch E., Rudolf G.,
RA Rosenberg D.S., Weber Y., Becker F., Helbig I., Muhle H., Salzmann A.,
RA Chaouch M., Oubaiche M.L., Ziglio S., Gehrig C., Santoni F., Pizzato M.,
RA Langel U., Antonarakis S.E.;
RT "Galanin pathogenic mutations in temporal lobe epilepsy.";
RL Hum. Mol. Genet. 24:3082-3091(2015).
CC -!- FUNCTION: Promotes matrix assembly. {ECO:0000250}.
CC -!- SUBUNIT: Binds to heparin, dermatan sulfate and chondroitin sulfate.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=1;
CC IsoId=Q9H0B8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9H0B8-2; Sequence=VSP_020183;
CC Name=3;
CC IsoId=Q9H0B8-3; Sequence=VSP_020181, VSP_020182;
CC Name=4;
CC IsoId=Q9H0B8-4; Sequence=VSP_020178, VSP_020180;
CC Name=5;
CC IsoId=Q9H0B8-5; Sequence=VSP_020179;
CC -!- SIMILARITY: Belongs to the CRISP family. {ECO:0000305}.
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DR EMBL; AL136861; CAB66795.1; -; mRNA.
DR EMBL; AY358413; AAQ88779.1; -; mRNA.
DR EMBL; AY358790; AAQ89150.1; -; mRNA.
DR EMBL; AK027395; BAB55081.1; -; mRNA.
DR EMBL; CH471114; EAW95468.1; -; Genomic_DNA.
DR EMBL; CH471114; EAW95469.1; -; Genomic_DNA.
DR EMBL; BC007689; AAH07689.1; -; mRNA.
DR EMBL; BC063012; AAH63012.1; -; mRNA.
DR CCDS; CCDS10949.1; -. [Q9H0B8-1]
DR RefSeq; NP_113664.1; NM_031476.3. [Q9H0B8-1]
DR RefSeq; XP_005256247.1; XM_005256190.1. [Q9H0B8-1]
DR AlphaFoldDB; Q9H0B8; -.
DR SMR; Q9H0B8; -.
DR BioGRID; 123739; 5.
DR IntAct; Q9H0B8; 1.
DR STRING; 9606.ENSP00000262424; -.
DR GlyGen; Q9H0B8; 5 sites, 2 O-linked glycans (3 sites).
DR iPTMnet; Q9H0B8; -.
DR PhosphoSitePlus; Q9H0B8; -.
DR BioMuta; CRISPLD2; -.
DR DMDM; 74717972; -.
DR jPOST; Q9H0B8; -.
DR MassIVE; Q9H0B8; -.
DR MaxQB; Q9H0B8; -.
DR PaxDb; Q9H0B8; -.
DR PeptideAtlas; Q9H0B8; -.
DR PRIDE; Q9H0B8; -.
DR ProteomicsDB; 80245; -. [Q9H0B8-1]
DR ProteomicsDB; 80246; -. [Q9H0B8-2]
DR ProteomicsDB; 80247; -. [Q9H0B8-3]
DR ProteomicsDB; 80248; -. [Q9H0B8-4]
DR ProteomicsDB; 80249; -. [Q9H0B8-5]
DR Antibodypedia; 30609; 139 antibodies from 23 providers.
DR DNASU; 83716; -.
DR Ensembl; ENST00000262424.10; ENSP00000262424.5; ENSG00000103196.12. [Q9H0B8-1]
DR Ensembl; ENST00000564567.5; ENSP00000457655.1; ENSG00000103196.12. [Q9H0B8-2]
DR Ensembl; ENST00000569090.1; ENSP00000454858.1; ENSG00000103196.12. [Q9H0B8-5]
DR GeneID; 83716; -.
DR KEGG; hsa:83716; -.
DR MANE-Select; ENST00000262424.10; ENSP00000262424.5; NM_031476.4; NP_113664.1.
DR UCSC; uc002fil.3; human. [Q9H0B8-1]
DR CTD; 83716; -.
DR DisGeNET; 83716; -.
DR GeneCards; CRISPLD2; -.
DR HGNC; HGNC:25248; CRISPLD2.
DR HPA; ENSG00000103196; Low tissue specificity.
DR MIM; 612434; gene.
DR neXtProt; NX_Q9H0B8; -.
DR OpenTargets; ENSG00000103196; -.
DR PharmGKB; PA142672077; -.
DR VEuPathDB; HostDB:ENSG00000103196; -.
DR eggNOG; KOG3017; Eukaryota.
DR GeneTree; ENSGT00940000157410; -.
DR HOGENOM; CLU_138283_0_0_1; -.
DR InParanoid; Q9H0B8; -.
DR OMA; VNTCQRM; -.
DR PhylomeDB; Q9H0B8; -.
DR TreeFam; TF316148; -.
DR PathwayCommons; Q9H0B8; -.
DR Reactome; R-HSA-6798695; Neutrophil degranulation.
DR SignaLink; Q9H0B8; -.
DR BioGRID-ORCS; 83716; 14 hits in 1070 CRISPR screens.
DR ChiTaRS; CRISPLD2; human.
DR GeneWiki; CRISPLD2; -.
DR GenomeRNAi; 83716; -.
DR Pharos; Q9H0B8; Tbio.
DR PRO; PR:Q9H0B8; -.
DR Proteomes; UP000005640; Chromosome 16.
DR RNAct; Q9H0B8; protein.
DR Bgee; ENSG00000103196; Expressed in decidua and 199 other tissues.
DR ExpressionAtlas; Q9H0B8; baseline and differential.
DR Genevisible; Q9H0B8; HS.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0031012; C:extracellular matrix; IEA:Ensembl.
DR GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:1904813; C:ficolin-1-rich granule lumen; TAS:Reactome.
DR GO; GO:0034774; C:secretory granule lumen; TAS:Reactome.
DR GO; GO:0030133; C:transport vesicle; IDA:LIFEdb.
DR GO; GO:0005539; F:glycosaminoglycan binding; IBA:GO_Central.
DR GO; GO:0008201; F:heparin binding; IEA:Ensembl.
DR GO; GO:0030198; P:extracellular matrix organization; IEA:Ensembl.
DR GO; GO:0060325; P:face morphogenesis; IMP:UniProtKB.
DR GO; GO:0030324; P:lung development; IEA:Ensembl.
DR Gene3D; 2.170.130.20; -; 2.
DR Gene3D; 3.40.33.10; -; 1.
DR InterPro; IPR018244; Allrgn_V5/Tpx1_CS.
DR InterPro; IPR014044; CAP_domain.
DR InterPro; IPR035940; CAP_sf.
DR InterPro; IPR001283; CRISP-related.
DR InterPro; IPR004043; LCCL.
DR InterPro; IPR036609; LCCL_sf.
DR PANTHER; PTHR10334; PTHR10334; 1.
DR Pfam; PF00188; CAP; 1.
DR Pfam; PF03815; LCCL; 2.
DR PRINTS; PR00837; V5TPXLIKE.
DR SMART; SM00603; LCCL; 2.
DR SMART; SM00198; SCP; 1.
DR SUPFAM; SSF55797; SSF55797; 1.
DR SUPFAM; SSF69848; SSF69848; 2.
DR PROSITE; PS01010; CRISP_2; 1.
DR PROSITE; PS50820; LCCL; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Disulfide bond; Glycoprotein; Reference proteome;
KW Repeat; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..497
FT /note="Cysteine-rich secretory protein LCCL domain-
FT containing 2"
FT /id="PRO_0000248149"
FT DOMAIN 62..200
FT /note="SCP"
FT DOMAIN 284..379
FT /note="LCCL 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00123"
FT DOMAIN 385..488
FT /note="LCCL 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00123"
FT CARBOHYD 27
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 290..308
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00123"
FT DISULFID 312..332
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00123"
FT DISULFID 391..413
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00123"
FT DISULFID 417..440
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00123"
FT VAR_SEQ 81..159
FT /note="TWDDELEKSAAAWASQCIWEHGPTSLLVSIGQNLGAHWGRYRSPGFHVQSWY
FT DEVKDYTYPYPSECNPWCPERCSGPMC -> VSAGSGRRGWHRGWGLGHQPALFPSQLC
FT SPASACDGWLRVSSGRGGSRLCSVLFVCFETGSHSATDAGVQWHNRHALKP (in
FT isoform 4)"
FT /evidence="ECO:0000303|PubMed:12975309"
FT /id="VSP_020178"
FT VAR_SEQ 121..497
FT /note="Missing (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_020179"
FT VAR_SEQ 160..497
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:12975309"
FT /id="VSP_020180"
FT VAR_SEQ 371
FT /note="S -> R (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_020181"
FT VAR_SEQ 372..497
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_020182"
FT VAR_SEQ 436..497
FT /note="TSSICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDG
FT KAFRIFAVRQ -> VSRMHFQQLSRKAC (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_020183"
FT VARIANT 105
FT /note="S -> G (in dbSNP:rs12051468)"
FT /evidence="ECO:0000269|PubMed:15489334, ECO:0000269|Ref.4"
FT /id="VAR_027256"
FT VARIANT 137
FT /note="D -> G (in dbSNP:rs1297496215)"
FT /evidence="ECO:0000269|PubMed:25691535"
FT /id="VAR_074672"
FT VARIANT 322
FT /note="T -> S (in dbSNP:rs721005)"
FT /evidence="ECO:0000269|PubMed:12975309,
FT ECO:0000269|PubMed:15489334"
FT /id="VAR_027257"
SQ SEQUENCE 497 AA; 55920 MW; 1E7ADDCAB37CA4ED CRC64;
MSCVLGGVIP LGLLFLVCGS QGYLLPNVTL LEELLSKYQH NESHSRVRRA IPREDKEEIL
MLHNKLRGQV QPQASNMEYM TWDDELEKSA AAWASQCIWE HGPTSLLVSI GQNLGAHWGR
YRSPGFHVQS WYDEVKDYTY PYPSECNPWC PERCSGPMCT HYTQIVWATT NKIGCAVNTC
RKMTVWGEVW ENAVYFVCNY SPKGNWIGEA PYKNGRPCSE CPPSYGGSCR NNLCYREETY
TPKPETDEMN EVETAPIPEE NHVWLQPRVM RPTKPKKTSA VNYMTQVVRC DTKMKDRCKG
STCNRYQCPA GCLNHKAKIF GTLFYESSSS ICRAAIHYGI LDDKGGLVDI TRNGKVPFFV
KSERHGVQSL SKYKPSSSFM VSKVKVQDLD CYTTVAQLCP FEKPATHCPR IHCPAHCKDE
PSYWAPVFGT NIYADTSSIC KTAVHAGVIS NESGGDVDVM PVDKKKTYVG SLRNGVQSES
LGTPRDGKAF RIFAVRQ
