CRLD2_MOUSE
ID CRLD2_MOUSE Reviewed; 495 AA.
AC Q8BZQ2; Q3U4C7; Q9D2R3;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Cysteine-rich secretory protein LCCL domain-containing 2;
DE AltName: Full=Coffeecrisp;
DE Flags: Precursor;
GN Name=Crispld2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Diencephalon, and Pancreas;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP DEVELOPMENTAL STAGE.
RX PubMed=17616516; DOI=10.1093/hmg/ddm176;
RA Chiquet B.T., Lidral A.C., Stal S., Mulliken J.B., Moreno L.M.,
RA Arcos-Burgos M., Valencia-Ramirez C., Blanton S.H., Hecht J.T.;
RT "CRISPLD2: a novel NSCLP candidate gene.";
RL Hum. Mol. Genet. 16:2241-2248(2007).
RN [5]
RP FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=18757743; DOI=10.1073/pnas.0803640105;
RA Manabe R., Tsutsui K., Yamada T., Kimura M., Nakano I., Shimono C.,
RA Sanzen N., Furutani Y., Fukuda T., Oguri Y., Shimamoto K., Kiyozumi D.,
RA Sato Y., Sado Y., Senoo H., Yamashina S., Fukuda S., Kawai J., Sugiura N.,
RA Kimata K., Hayashizaki Y., Sekiguchi K.;
RT "Transcriptome-based systematic identification of extracellular matrix
RT proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:12849-12854(2008).
CC -!- FUNCTION: Promotes matrix assembly. {ECO:0000269|PubMed:18757743}.
CC -!- SUBUNIT: Binds to heparin, dermatan sulfate and chondroitin sulfate.
CC {ECO:0000269|PubMed:18757743}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18757743}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8BZQ2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BZQ2-2; Sequence=VSP_036331;
CC -!- TISSUE SPECIFICITY: Present in kidney renal tubules (at protein level).
CC {ECO:0000269|PubMed:18757743}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the developing oropharynx and
CC nasopharynx at 13.5 dpc, in the mandible at 14.5 dpc and in the
CC cartilage primordia of the nasal bones, palate and tooth germs at 17.5
CC dpc. Detected in the embryonic liver at 13.5, 14.5 and 17.5 dpc.
CC {ECO:0000269|PubMed:17616516}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK019034; BAB31519.2; -; mRNA.
DR EMBL; AK033858; BAC28498.1; -; mRNA.
DR EMBL; AK154308; BAE32505.1; -; mRNA.
DR EMBL; CH466525; EDL11621.1; -; Genomic_DNA.
DR EMBL; CH466525; EDL11622.1; -; Genomic_DNA.
DR EMBL; BC090642; AAH90642.1; -; mRNA.
DR CCDS; CCDS22714.1; -. [Q8BZQ2-2]
DR CCDS; CCDS80939.1; -. [Q8BZQ2-1]
DR RefSeq; NP_001297564.1; NM_001310635.1. [Q8BZQ2-1]
DR RefSeq; NP_084485.1; NM_030209.4. [Q8BZQ2-2]
DR RefSeq; XP_006531571.1; XM_006531508.1. [Q8BZQ2-1]
DR RefSeq; XP_006531572.1; XM_006531509.1. [Q8BZQ2-2]
DR AlphaFoldDB; Q8BZQ2; -.
DR SMR; Q8BZQ2; -.
DR BioGRID; 219687; 1.
DR STRING; 10090.ENSMUSP00000122962; -.
DR GlyGen; Q8BZQ2; 1 site.
DR iPTMnet; Q8BZQ2; -.
DR PhosphoSitePlus; Q8BZQ2; -.
DR CPTAC; non-CPTAC-3455; -.
DR MaxQB; Q8BZQ2; -.
DR PaxDb; Q8BZQ2; -.
DR PeptideAtlas; Q8BZQ2; -.
DR PRIDE; Q8BZQ2; -.
DR ProteomicsDB; 285334; -. [Q8BZQ2-1]
DR ProteomicsDB; 285335; -. [Q8BZQ2-2]
DR Antibodypedia; 30609; 139 antibodies from 23 providers.
DR DNASU; 78892; -.
DR Ensembl; ENSMUST00000034282; ENSMUSP00000034282; ENSMUSG00000031825. [Q8BZQ2-1]
DR Ensembl; ENSMUST00000108972; ENSMUSP00000104600; ENSMUSG00000031825. [Q8BZQ2-2]
DR Ensembl; ENSMUST00000132583; ENSMUSP00000122962; ENSMUSG00000031825. [Q8BZQ2-2]
DR GeneID; 78892; -.
DR KEGG; mmu:78892; -.
DR UCSC; uc009nqq.2; mouse. [Q8BZQ2-2]
DR UCSC; uc012glv.1; mouse. [Q8BZQ2-1]
DR CTD; 83716; -.
DR MGI; MGI:1926142; Crispld2.
DR VEuPathDB; HostDB:ENSMUSG00000031825; -.
DR eggNOG; KOG3017; Eukaryota.
DR GeneTree; ENSGT00940000157410; -.
DR HOGENOM; CLU_042287_0_0_1; -.
DR InParanoid; Q8BZQ2; -.
DR OMA; VNTCQRM; -.
DR OrthoDB; 1528782at2759; -.
DR PhylomeDB; Q8BZQ2; -.
DR TreeFam; TF316148; -.
DR Reactome; R-MMU-6798695; Neutrophil degranulation.
DR BioGRID-ORCS; 78892; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Crispld2; mouse.
DR PRO; PR:Q8BZQ2; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q8BZQ2; protein.
DR Bgee; ENSMUSG00000031825; Expressed in iris and 171 other tissues.
DR Genevisible; Q8BZQ2; MM.
DR GO; GO:0031012; C:extracellular matrix; IDA:MGI.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0030133; C:transport vesicle; ISO:MGI.
DR GO; GO:0005539; F:glycosaminoglycan binding; IDA:MGI.
DR GO; GO:0008201; F:heparin binding; IDA:MGI.
DR GO; GO:0030198; P:extracellular matrix organization; IDA:MGI.
DR GO; GO:0060325; P:face morphogenesis; ISO:MGI.
DR GO; GO:0030324; P:lung development; IEA:Ensembl.
DR Gene3D; 2.170.130.20; -; 2.
DR Gene3D; 3.40.33.10; -; 1.
DR InterPro; IPR018244; Allrgn_V5/Tpx1_CS.
DR InterPro; IPR014044; CAP_domain.
DR InterPro; IPR035940; CAP_sf.
DR InterPro; IPR001283; CRISP-related.
DR InterPro; IPR004043; LCCL.
DR InterPro; IPR036609; LCCL_sf.
DR PANTHER; PTHR10334; PTHR10334; 1.
DR Pfam; PF00188; CAP; 1.
DR Pfam; PF03815; LCCL; 2.
DR PRINTS; PR00837; V5TPXLIKE.
DR SMART; SM00603; LCCL; 2.
DR SMART; SM00198; SCP; 1.
DR SUPFAM; SSF55797; SSF55797; 1.
DR SUPFAM; SSF69848; SSF69848; 2.
DR PROSITE; PS01010; CRISP_2; 1.
DR PROSITE; PS50820; LCCL; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Disulfide bond; Glycoprotein; Reference proteome;
KW Repeat; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..495
FT /note="Cysteine-rich secretory protein LCCL domain-
FT containing 2"
FT /id="PRO_0000363885"
FT DOMAIN 60..200
FT /note="SCP"
FT DOMAIN 282..377
FT /note="LCCL 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00123"
FT DOMAIN 383..486
FT /note="LCCL 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00123"
FT CARBOHYD 27
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 288..306
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00123"
FT DISULFID 310..330
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00123"
FT DISULFID 389..411
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00123"
FT DISULFID 415..438
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00123"
FT VAR_SEQ 237
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_036331"
FT CONFLICT 55
FT /note="D -> G (in Ref. 1; BAE32505)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 495 AA; 55432 MW; 7F6D25DDC489610C CRC64;
MSCLLNNMVL MGLALLVCGV QAFFLPNTTS LEKLLSKYQH AEPHSRVRRA IPMSDRQEIL
MLHNKLRGQV YPPASNMEHM TWDEELERSA AAWAHRCLWE HGPAGLLRSI GQNLAVHWGR
YRSPGFHVQS WYDEVKDYTY PYPHECTPRC RERCSGPMCT HYTQMVWATT NKIGCAVHTC
RNMNVWGDTW ENAVYLVCNY SPKGNWIGEA PYKHGRPCSE CPSSYGGGCL NNLCHRAEKP
HKHKPEVDMM NEVESPPAPE ETHVWVQPRV IKTKKTPVIN FMTQVVHCDT KMKDSCKGST
CNRYQCPAGC LSNKAKVFGS LFYESSSSIC RAAIHYGVID DRGGLVDVTR NGMVPFFVKS
QKNGMESLSK YKPSSSFTVS KVTETAVDCH ATVAQLCPFE KPATHCPRIQ CPARCGEEPS
YWAPVYGTNI YADTSSICKA AVHAGVIVDE VGGYADVMPV DKKKSYVGSL RNGVQSESLN
TPQNGNAFRI FAVRQ
