ID   CRL_ECO8A               Reviewed;         133 AA.
AC   B7M269;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=Sigma factor-binding protein Crl {ECO:0000255|HAMAP-Rule:MF_01178};
GN   Name=crl {ECO:0000255|HAMAP-Rule:MF_01178}; OrderedLocusNames=ECIAI1_0279;
OS   Escherichia coli O8 (strain IAI1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=585034;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IAI1;
RX   PubMed=19165319; DOI=10.1371/journal.pgen.1000344;
RA   Touchon M., Hoede C., Tenaillon O., Barbe V., Baeriswyl S., Bidet P.,
RA   Bingen E., Bonacorsi S., Bouchier C., Bouvet O., Calteau A., Chiapello H.,
RA   Clermont O., Cruveiller S., Danchin A., Diard M., Dossat C., Karoui M.E.,
RA   Frapy E., Garry L., Ghigo J.M., Gilles A.M., Johnson J., Le Bouguenec C.,
RA   Lescat M., Mangenot S., Martinez-Jehanne V., Matic I., Nassif X., Oztas S.,
RA   Petit M.A., Pichon C., Rouy Z., Ruf C.S., Schneider D., Tourret J.,
RA   Vacherie B., Vallenet D., Medigue C., Rocha E.P.C., Denamur E.;
RT   "Organised genome dynamics in the Escherichia coli species results in
RT   highly diverse adaptive paths.";
RL   PLoS Genet. 5:E1000344-E1000344(2009).
CC   -!- FUNCTION: Binds to the sigma-S subunit of RNA polymerase, activating
CC       expression of sigma-S-regulated genes. Stimulates RNA polymerase
CC       holoenzyme formation and may bind to several other sigma factors, such
CC       as sigma-70 and sigma-32. {ECO:0000255|HAMAP-Rule:MF_01178}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01178}.
CC   -!- SIMILARITY: Belongs to the Crl family. {ECO:0000255|HAMAP-
CC       Rule:MF_01178}.
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DR   EMBL; CU928160; CAQ97153.1; -; Genomic_DNA.
DR   RefSeq; WP_000174677.1; NC_011741.1.
DR   AlphaFoldDB; B7M269; -.
DR   SMR; B7M269; -.
DR   GeneID; 66671442; -.
DR   KEGG; ecr:ECIAI1_0279; -.
DR   HOGENOM; CLU_136773_0_0_6; -.
DR   OMA; FWGWWLE; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.310.230; -; 1.
DR   HAMAP; MF_01178; Crl; 1.
DR   InterPro; IPR009986; Tscrpt_reg_Crl.
DR   InterPro; IPR038208; Tscrpt_reg_Crl_sf.
DR   Pfam; PF07417; Crl; 1.
PE   3: Inferred from homology;
KW   Activator; Coiled coil; Cytoplasm; Transcription; Transcription regulation.
FT   CHAIN           1..133
FT                   /note="Sigma factor-binding protein Crl"
FT                   /id="PRO_1000138137"
FT   REGION          99..122
FT                   /note="Essential for activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01178"
FT   COILED          90..116
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01178"
SQ   SEQUENCE   133 AA;  15656 MW;  E706CC43474EE67C CRC64;
     MTLPSGHPKS RLIKKFTALG PYIREGKCED NRFFFDCLAV CVNVKPAPEV REFWGWWMEL
     EAQESRFTYS YQFGLFDKAG DWKSVPVKDT EVVERLEHTL REFHEKLREL LTTLNLKLEP
     ADDFRDEPVK LTA