CRL_ECOLI
ID CRL_ECOLI Reviewed; 133 AA.
AC P24251; P77629; Q47242; Q47585;
DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 5.
DT 25-MAY-2022, entry version 149.
DE RecName: Full=Sigma factor-binding protein Crl;
DE AltName: Full=Curlin genes transcriptional activatory protein;
GN Name=crl; OrderedLocusNames=b0240, JW0230;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=6341601; DOI=10.1016/0022-2836(83)90110-9;
RA Overbeeke N., Bergmans H., van Mansfeld F., Lugtenberg B.;
RT "Complete nucleotide sequence of phoE, the structural gene for the
RT phosphate limitation inducible outer membrane pore protein of Escherichia
RT coli K12.";
RL J. Mol. Biol. 163:513-532(1983).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-6, AND
RP CHARACTERIZATION.
RC STRAIN=AO12;
RX PubMed=2649795; DOI=10.1038/338652a0;
RA Olsen A., Jonsson A., Normark S.;
RT "Fibronectin binding mediated by a novel class of surface organelles on
RT Escherichia coli.";
RL Nature 338:652-655(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=AO12;
RX PubMed=1357528; DOI=10.1111/j.1365-2958.1992.tb01420.x;
RA Arnqvist A., Olsen A., Pfeifer J., Russell D.G., Normark S.;
RT "The Crl protein activates cryptic genes for curli formation and
RT fibronectin binding in Escherichia coli HB101.";
RL Mol. Microbiol. 6:2443-2452(1992).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T.,
RA Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S.,
RA Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.;
RT "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0
RT - 6.0 min (189,987 - 281,416bp) region.";
RL Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [7]
RP SEQUENCE REVISION.
RC STRAIN=K12 / MG1655 / ATCC 700926;
RX PubMed=22081388; DOI=10.1128/jb.06087-11;
RA Freddolino P.L., Amini S., Tavazoie S.;
RT "Newly identified genetic variations in common Escherichia coli MG1655
RT stock cultures.";
RL J. Bacteriol. 194:303-306(2012).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO 28.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [9]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
RX PubMed=6397401; DOI=10.1016/0378-1119(84)90052-0;
RA Nueesch J., Schuemperli D.;
RT "Structural and functional organization of the gpt gene region of
RT Escherichia coli.";
RL Gene 32:243-249(1984).
RN [10]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
RC STRAIN=O9:K30:H12;
RX PubMed=1729226; DOI=10.1128/jb.174.1.8-16.1992;
RA Keenleyside W.J., Jayaratne P., MacLachlan P.R., Whitfield C.;
RT "The rcsA gene of Escherichia coli 09:K30:H12 is involved in the expression
RT of the serotype-specific group I K (capsular) antigen.";
RL J. Bacteriol. 174:8-16(1992).
RN [11]
RP ACTIVATION OF RPOS ACTIVITY.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=9767590; DOI=10.1046/j.1365-2958.1998.01007.x;
RA Pratt L.A., Silhavy T.J.;
RT "Crl stimulates RpoS activity during stationary phase.";
RL Mol. Microbiol. 29:1225-1236(1998).
RN [12]
RP BINDING TO SIGMA-SUBUNIT OF RNA POLYMERASE.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=14978043; DOI=10.1074/jbc.m314145200;
RA Bougdour A., Lelong C., Geiselmann J.;
RT "Crl, a low temperature-induced protein in Escherichia coli that binds
RT directly to the stationary phase sigma subunit of RNA polymerase.";
RL J. Biol. Chem. 279:19540-19550(2004).
RN [13]
RP ROLE IN RNA POLYMERASE HOLOENZYME FORMATION.
RX PubMed=16980472; DOI=10.1128/jb.01266-06;
RA Gaal T., Mandel M.J., Silhavy T.J., Gourse R.L.;
RT "Crl facilitates RNA polymerase holoenzyme formation.";
RL J. Bacteriol. 188:7966-7970(2006).
CC -!- FUNCTION: Binds to the sigma-S subunit of RNA polymerase, activating
CC expression of sigma-S-regulated genes, such as the csgBAC operon
CC encoding the subunits of curli proteins. Stimulates RNA polymerase
CC holoenzyme formation and may bind to several other sigma factors, such
CC as sigma-70 (rpoD) and sigma-32 (rpoH). {ECO:0000269|PubMed:1357528,
CC ECO:0000269|PubMed:16980472}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- INDUCTION: Induced at stationary phase, and by low temperatures.
CC -!- SIMILARITY: Belongs to the Crl family. {ECO:0000305}.
CC -!- CAUTION: A pseudogene in strain MG1655 / ATCC 700926, but not its
CC parent MG1655 / ATCC 47076. In ATCC 700926 IS1I has recently inserted
CC into the gene (PubMed:22081388). {ECO:0000305|PubMed:22081388}.
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DR EMBL; V00316; CAA23606.1; -; Genomic_DNA.
DR EMBL; X67207; CAA47646.1; -; Genomic_DNA.
DR EMBL; U70214; AAB08660.1; -; Genomic_DNA.
DR EMBL; U00096; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AP009048; BAA77909.2; -; Genomic_DNA.
DR EMBL; M13422; AAA23930.1; -; Genomic_DNA.
DR PIR; A64749; A64749.
DR RefSeq; WP_000174689.1; NZ_LN832404.1.
DR AlphaFoldDB; P24251; -.
DR SMR; P24251; -.
DR BioGRID; 4260821; 13.
DR IntAct; P24251; 11.
DR STRING; 316407.85674393; -.
DR jPOST; P24251; -.
DR PaxDb; P24251; -.
DR PRIDE; P24251; -.
DR EnsemblBacteria; BAA77909; BAA77909; BAA77909.
DR KEGG; ecj:JW0230; -.
DR PATRIC; fig|1411691.4.peg.2043; -.
DR EchoBASE; EB1084; -.
DR eggNOG; ENOG502ZQ8E; Bacteria.
DR HOGENOM; CLU_136773_0_0_6; -.
DR OMA; FWGWWLE; -.
DR PhylomeDB; P24251; -.
DR PRO; PR:P24251; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.310.230; -; 1.
DR HAMAP; MF_01178; Crl; 1.
DR InterPro; IPR009986; Tscrpt_reg_Crl.
DR InterPro; IPR038208; Tscrpt_reg_Crl_sf.
DR Pfam; PF07417; Crl; 1.
PE 1: Evidence at protein level;
KW Activator; Coiled coil; Cytoplasm; Direct protein sequencing;
KW Reference proteome; Transcription; Transcription regulation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:2649795"
FT CHAIN 2..133
FT /note="Sigma factor-binding protein Crl"
FT /id="PRO_0000079355"
FT REGION 99..122
FT /note="Essential for activity"
FT COILED 90..116
FT /evidence="ECO:0000255"
FT CONFLICT 8
FT /note="P -> L (in Ref. 1; CAA23606)"
FT /evidence="ECO:0000305"
FT CONFLICT 13
FT /note="I -> N (in Ref. 1; CAA23606)"
FT /evidence="ECO:0000305"
FT CONFLICT 29
FT /note="K -> E (in Ref. 2 and 3; CAA47646)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 133 AA; 15655 MW; EA66172EF19586AA CRC64;
MTLPSGHPKS RLIKKFTALG PYIREGKCKD NRFFFDCLAV CVNVKPAPEV REFWGWWMEL
EAQESRFTYS YQFGLFDKAG DWKSVPVKDT EVVERLEHTL REFHEKLREL LTTLNLKLEP
ADDFRDEPVK LTA
