ID   CRL_PECAS               Reviewed;         124 AA.
AC   Q6D1I2;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Sigma factor-binding protein Crl {ECO:0000255|HAMAP-Rule:MF_01178};
GN   Name=crl {ECO:0000255|HAMAP-Rule:MF_01178}; OrderedLocusNames=ECA3464;
OS   Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia
OS   carotovora subsp. atroseptica).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Pectobacteriaceae; Pectobacterium.
OX   NCBI_TaxID=218491;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SCRI 1043 / ATCC BAA-672;
RX   PubMed=15263089; DOI=10.1073/pnas.0402424101;
RA   Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J.,
RA   Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K.,
RA   Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J.,
RA   Fraser A., Hance Z., Hauser H., Jagels K., Moule S., Norbertczak H.,
RA   Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S.,
RA   Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K.;
RT   "Genome sequence of the enterobacterial phytopathogen Erwinia carotovora
RT   subsp. atroseptica and characterization of virulence factors.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004).
CC   -!- FUNCTION: Binds to the sigma-S subunit of RNA polymerase, activating
CC       expression of sigma-S-regulated genes. Stimulates RNA polymerase
CC       holoenzyme formation and may bind to several other sigma factors, such
CC       as sigma-70 and sigma-32. {ECO:0000255|HAMAP-Rule:MF_01178}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01178}.
CC   -!- SIMILARITY: Belongs to the Crl family. {ECO:0000255|HAMAP-
CC       Rule:MF_01178}.
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DR   EMBL; BX950851; CAG76363.1; -; Genomic_DNA.
DR   RefSeq; WP_011094972.1; NC_004547.2.
DR   AlphaFoldDB; Q6D1I2; -.
DR   SMR; Q6D1I2; -.
DR   STRING; 218491.ECA3464; -.
DR   EnsemblBacteria; CAG76363; CAG76363; ECA3464.
DR   GeneID; 57210133; -.
DR   KEGG; eca:ECA3464; -.
DR   PATRIC; fig|218491.5.peg.3504; -.
DR   eggNOG; ENOG502ZQ8E; Bacteria.
DR   HOGENOM; CLU_136773_0_0_6; -.
DR   OMA; FWGWWLE; -.
DR   OrthoDB; 1914214at2; -.
DR   Proteomes; UP000007966; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.310.230; -; 1.
DR   HAMAP; MF_01178; Crl; 1.
DR   InterPro; IPR009986; Tscrpt_reg_Crl.
DR   InterPro; IPR038208; Tscrpt_reg_Crl_sf.
DR   Pfam; PF07417; Crl; 1.
PE   3: Inferred from homology;
KW   Activator; Coiled coil; Cytoplasm; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..124
FT                   /note="Sigma factor-binding protein Crl"
FT                   /id="PRO_0000268895"
FT   REGION          99..118
FT                   /note="Essential for activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01178"
FT   COILED          90..115
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01178"
SQ   SEQUENCE   124 AA;  14427 MW;  0FC5766D0AEA5167 CRC64;
     MMLPSGHPKS RLMKNFTALG PYIREAQCED TAFFFDCLAV CVNIKPAPEE REFWGWWLNL
     EDTGKAFSYE YHYGLFDKQG NWREEKIKGK AVIEQVENAK QAFHVRLEKL LRSLEPACTL
     VARP