ID   CRL_SHIF8               Reviewed;         133 AA.
AC   Q0T7R1;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Sigma factor-binding protein Crl {ECO:0000255|HAMAP-Rule:MF_01178};
GN   Name=crl {ECO:0000255|HAMAP-Rule:MF_01178}; OrderedLocusNames=SFV_0290;
OS   Shigella flexneri serotype 5b (strain 8401).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=373384;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=8401;
RX   PubMed=16822325; DOI=10.1186/1471-2164-7-173;
RA   Nie H., Yang F., Zhang X., Yang J., Chen L., Wang J., Xiong Z., Peng J.,
RA   Sun L., Dong J., Xue Y., Xu X., Chen S., Yao Z., Shen Y., Jin Q.;
RT   "Complete genome sequence of Shigella flexneri 5b and comparison with
RT   Shigella flexneri 2a.";
RL   BMC Genomics 7:173-173(2006).
CC   -!- FUNCTION: Binds to the sigma-S subunit of RNA polymerase, activating
CC       expression of sigma-S-regulated genes. Stimulates RNA polymerase
CC       holoenzyme formation and may bind to several other sigma factors, such
CC       as sigma-70 and sigma-32. {ECO:0000255|HAMAP-Rule:MF_01178}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01178}.
CC   -!- SIMILARITY: Belongs to the Crl family. {ECO:0000255|HAMAP-
CC       Rule:MF_01178}.
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DR   EMBL; CP000266; ABF02565.1; -; Genomic_DNA.
DR   RefSeq; WP_000174677.1; NC_008258.1.
DR   AlphaFoldDB; Q0T7R1; -.
DR   SMR; Q0T7R1; -.
DR   EnsemblBacteria; ABF02565; ABF02565; SFV_0290.
DR   GeneID; 66671442; -.
DR   KEGG; sfv:SFV_0290; -.
DR   HOGENOM; CLU_136773_0_0_6; -.
DR   OMA; FWGWWLE; -.
DR   BioCyc; SFLE373384:SFV_RS01620-MON; -.
DR   Proteomes; UP000000659; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.310.230; -; 1.
DR   HAMAP; MF_01178; Crl; 1.
DR   InterPro; IPR009986; Tscrpt_reg_Crl.
DR   InterPro; IPR038208; Tscrpt_reg_Crl_sf.
DR   Pfam; PF07417; Crl; 1.
PE   3: Inferred from homology;
KW   Activator; Coiled coil; Cytoplasm; Transcription; Transcription regulation.
FT   CHAIN           1..133
FT                   /note="Sigma factor-binding protein Crl"
FT                   /id="PRO_1000065789"
FT   REGION          99..122
FT                   /note="Essential for activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01178"
FT   COILED          90..116
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01178"
SQ   SEQUENCE   133 AA;  15656 MW;  E706CC43474EE67C CRC64;
     MTLPSGHPKS RLIKKFTALG PYIREGKCED NRFFFDCLAV CVNVKPAPEV REFWGWWMEL
     EAQESRFTYS YQFGLFDKAG DWKSVPVKDT EVVERLEHTL REFHEKLREL LTTLNLKLEP
     ADDFRDEPVK LTA